Tags

Type your tag names separated by a space and hit enter

Kinetics of tumour necrosis factor and prostaglandin production by murine resident peritoneal macrophages as affected by dietary n-3 polyunsaturated fatty acids.
Immunology 1992; 76(4):572-7I

Abstract

Cell-associated and secreted tumour necrosis factor (TNF), prostaglandin (PG) E2, and 6-keto PGF1 alpha were monitored at various times following in vitro stimulation of resident peritoneal macrophages with lipopolysaccharide (LPS). Macrophages were obtained from mice maintained on diets containing 1.5 wt% n-3 polyunsaturated fatty acids (PUFA)+ 1.5 wt% n-6 fatty acids; 1.5 wt% n-6 fatty acids; or 3 wt% n-6 fatty acids, for 4 weeks. Cell-associated TNF increased transiently in the resident peritoneal macrophages from mice consuming all diets and decreased after TNF secretion had reached maximum and plateaued. Macrophages from mice consuming the n-3 PUFA contained more cell-associated TNF and secreted more TNF than macrophages from mice consuming diets containing n-6 fatty acids only, at all time-points studied. Macrophages from mice consuming the n-3 PUFA showed an earlier increase in cell-associated and secreted TNF compared with macrophages from mice consuming n-6 fatty acids only. Kinetics of maximum TNF production was not affected by the diets and dietary n-3 PUFA did not cause a prolonged increase in TNF secretion. Macrophages from mice consuming the n-3 PUFA produced less PG than macrophages from mice consuming the n-6 fatty acids only. PG secretion increased following appearance of cell-associated TNF but when PG had accumulated in the medium there was no further increase in TNF production.

Authors+Show Affiliations

Lipids Research Laboratory, Cornell University, Ithaca, New York.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1398747

Citation

Hardard'ottir, I, et al. "Kinetics of Tumour Necrosis Factor and Prostaglandin Production By Murine Resident Peritoneal Macrophages as Affected By Dietary N-3 Polyunsaturated Fatty Acids." Immunology, vol. 76, no. 4, 1992, pp. 572-7.
Hardard'ottir I, Whelan J, Kinsella JE. Kinetics of tumour necrosis factor and prostaglandin production by murine resident peritoneal macrophages as affected by dietary n-3 polyunsaturated fatty acids. Immunology. 1992;76(4):572-7.
Hardard'ottir, I., Whelan, J., & Kinsella, J. E. (1992). Kinetics of tumour necrosis factor and prostaglandin production by murine resident peritoneal macrophages as affected by dietary n-3 polyunsaturated fatty acids. Immunology, 76(4), pp. 572-7.
Hardard'ottir I, Whelan J, Kinsella JE. Kinetics of Tumour Necrosis Factor and Prostaglandin Production By Murine Resident Peritoneal Macrophages as Affected By Dietary N-3 Polyunsaturated Fatty Acids. Immunology. 1992;76(4):572-7. PubMed PMID: 1398747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kinetics of tumour necrosis factor and prostaglandin production by murine resident peritoneal macrophages as affected by dietary n-3 polyunsaturated fatty acids. AU - Hardard'ottir,I, AU - Whelan,J, AU - Kinsella,J E, PY - 1992/8/1/pubmed PY - 1992/8/1/medline PY - 1992/8/1/entrez SP - 572 EP - 7 JF - Immunology JO - Immunology VL - 76 IS - 4 N2 - Cell-associated and secreted tumour necrosis factor (TNF), prostaglandin (PG) E2, and 6-keto PGF1 alpha were monitored at various times following in vitro stimulation of resident peritoneal macrophages with lipopolysaccharide (LPS). Macrophages were obtained from mice maintained on diets containing 1.5 wt% n-3 polyunsaturated fatty acids (PUFA)+ 1.5 wt% n-6 fatty acids; 1.5 wt% n-6 fatty acids; or 3 wt% n-6 fatty acids, for 4 weeks. Cell-associated TNF increased transiently in the resident peritoneal macrophages from mice consuming all diets and decreased after TNF secretion had reached maximum and plateaued. Macrophages from mice consuming the n-3 PUFA contained more cell-associated TNF and secreted more TNF than macrophages from mice consuming diets containing n-6 fatty acids only, at all time-points studied. Macrophages from mice consuming the n-3 PUFA showed an earlier increase in cell-associated and secreted TNF compared with macrophages from mice consuming n-6 fatty acids only. Kinetics of maximum TNF production was not affected by the diets and dietary n-3 PUFA did not cause a prolonged increase in TNF secretion. Macrophages from mice consuming the n-3 PUFA produced less PG than macrophages from mice consuming the n-6 fatty acids only. PG secretion increased following appearance of cell-associated TNF but when PG had accumulated in the medium there was no further increase in TNF production. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/1398747/Kinetics_of_tumour_necrosis_factor_and_prostaglandin_production_by_murine_resident_peritoneal_macrophages_as_affected_by_dietary_n_3_polyunsaturated_fatty_acids_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/1398747/ DB - PRIME DP - Unbound Medicine ER -