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Separation and identification of carotenoids and their oxidation products in the extracts of human plasma.
Anal Chem. 1992 Sep 15; 64(18):2111-22.AC

Abstract

Eighteen carotenoids as well as vitamin A and two forms of vitamin E (gamma- and alpha-tocopherol) have been separated from extracts of human plasma by high-performance liquid chromatography (HPLC) on reversed-phase and sillca-based nitrile-bonded columns. In the order of chromatographic elution on a C18 reversed-phase column, the carotenoids were identified as (3R,3'R,6'R)-beta, epsilon-carotene-3,3'-diol [(3R,3'R,6'R)-lutein], (3R,3'R)-beta, beta-carotene-3,3'-diol [(3R,3'R)-zeaxanthin], 5,6-dihydroxy-5,6-dihydro-psi,psi-carotene, 3-hydroxy-2',3'-didehydro-beta,epsilon-caroten-3-ol, 3-hydroxy-beta-carotene,psi,psi-carotene, 7,8-dihydro-psi,psi-carotene, beta,psi-carotene, 7,8,7',8'-tetrahydro-psi,psi-carotene, beta,epsilon-carotene, beta,beta-carotene, 7,8,11,12,7',8'-hexahydro-psi,psi-carotene, and 7,8,11,12,7',8'-11',12'-octahydro-psi,psi-carotene. The polar carotenoids, which eluted in the vicinity of lutein and were unresolved on the C18 column, have been separated on a nitrile-bonded column employing isocratic HPLC conditions. In the order of elution, the carotenoids were epsilon,epsilon-carotene-3,3'-dione, 3'-hydroxy-epsilon,epsilon-caroten-3-one, 5,6-dihydroxy-5,6-dihydro-psi,psi-carotene, 3-hydroxy-beta,epsilon-caroten-3'-one, (all-E,3R,3'R,6'R)-lutein, (all-E,3R,3'R)-zeaxanthin, and (all-E,3R,3'S,6'R)-beta,epsilon-carotene-3,3'-diol (3'-epilutein) followed by several geometrical isomers of lutein and zeaxanthin.

Authors+Show Affiliations

Nutrient Composition Laboratory, U.S. Department of Agriculture, ARS, Beltsville, Maryland 20705.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1416048

Citation

Khachik, F, et al. "Separation and Identification of Carotenoids and Their Oxidation Products in the Extracts of Human Plasma." Analytical Chemistry, vol. 64, no. 18, 1992, pp. 2111-22.
Khachik F, Beecher GR, Goli MB, et al. Separation and identification of carotenoids and their oxidation products in the extracts of human plasma. Anal Chem. 1992;64(18):2111-22.
Khachik, F., Beecher, G. R., Goli, M. B., Lusby, W. R., & Smith, J. C. (1992). Separation and identification of carotenoids and their oxidation products in the extracts of human plasma. Analytical Chemistry, 64(18), 2111-22.
Khachik F, et al. Separation and Identification of Carotenoids and Their Oxidation Products in the Extracts of Human Plasma. Anal Chem. 1992 Sep 15;64(18):2111-22. PubMed PMID: 1416048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and identification of carotenoids and their oxidation products in the extracts of human plasma. AU - Khachik,F, AU - Beecher,G R, AU - Goli,M B, AU - Lusby,W R, AU - Smith,J C,Jr PY - 1992/9/25/pubmed PY - 2001/3/28/medline PY - 1992/9/25/entrez SP - 2111 EP - 22 JF - Analytical chemistry JO - Anal. Chem. VL - 64 IS - 18 N2 - Eighteen carotenoids as well as vitamin A and two forms of vitamin E (gamma- and alpha-tocopherol) have been separated from extracts of human plasma by high-performance liquid chromatography (HPLC) on reversed-phase and sillca-based nitrile-bonded columns. In the order of chromatographic elution on a C18 reversed-phase column, the carotenoids were identified as (3R,3'R,6'R)-beta, epsilon-carotene-3,3'-diol [(3R,3'R,6'R)-lutein], (3R,3'R)-beta, beta-carotene-3,3'-diol [(3R,3'R)-zeaxanthin], 5,6-dihydroxy-5,6-dihydro-psi,psi-carotene, 3-hydroxy-2',3'-didehydro-beta,epsilon-caroten-3-ol, 3-hydroxy-beta-carotene,psi,psi-carotene, 7,8-dihydro-psi,psi-carotene, beta,psi-carotene, 7,8,7',8'-tetrahydro-psi,psi-carotene, beta,epsilon-carotene, beta,beta-carotene, 7,8,11,12,7',8'-hexahydro-psi,psi-carotene, and 7,8,11,12,7',8'-11',12'-octahydro-psi,psi-carotene. The polar carotenoids, which eluted in the vicinity of lutein and were unresolved on the C18 column, have been separated on a nitrile-bonded column employing isocratic HPLC conditions. In the order of elution, the carotenoids were epsilon,epsilon-carotene-3,3'-dione, 3'-hydroxy-epsilon,epsilon-caroten-3-one, 5,6-dihydroxy-5,6-dihydro-psi,psi-carotene, 3-hydroxy-beta,epsilon-caroten-3'-one, (all-E,3R,3'R,6'R)-lutein, (all-E,3R,3'R)-zeaxanthin, and (all-E,3R,3'S,6'R)-beta,epsilon-carotene-3,3'-diol (3'-epilutein) followed by several geometrical isomers of lutein and zeaxanthin. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/1416048/Separation_and_identification_of_carotenoids_and_their_oxidation_products_in_the_extracts_of_human_plasma_ L2 - https://www.lens.org/lens/search?q=citation_id:1416048 DB - PRIME DP - Unbound Medicine ER -