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Washing erythrocytes to remove interferents in measurements of zinc protoporphyrin by front-face hematofluorometry.
Clin Chem. 1992 Nov; 38(11):2184-9.CC

Abstract

Zinc protoporphyrin (ZPP) is determined by hematofluorometry of whole blood to detect iron deficiency in blood donors. In hospitalized patients, ZPP did not correlate with established markers of iron status. We performed 4500 ZPP measurements with the Aviv front-face hematofluorometer in samples from 475 patients and measured ferritin, transferrin saturation, hemoglobin, and erythrocyte indices. We found that the fluorometric determination is affected by substances dissolved in plasma but that this interference can be eliminated by using washed erythrocytes. In validation tests the within-day variation was < 3.5%; the day-to-day variation was < 6.8%. In 130 healthy persons without iron deficiency, ZPP was < or = 40 mumol/mol heme, which we consider a normal value. Mean ZPP in 46 iron-deficient patients was 256 (SD 105) mumol/mol heme (correlation with ferritin: -0.73; with hemoglobin: -0.85; P < 0.001). When washed erythrocytes are used, the hematofluorometric determination of ZPP is sensitive and specific for detecting iron deficiency in otherwise healthy individuals and hospitalized patients.

Authors+Show Affiliations

Hastka J
III Medizin Klinik, University of Heidelberg, Germany.
Lasserre JJ
No affiliation info available
Schwarzbeck A
No affiliation info available
Strauch M
No affiliation info available
Hehlmann R
No affiliation info available

MeSH

Blood DonorsErythrocyte IndicesErythrocytesFerritinsFluorometryHemoglobinsHumansIron DeficienciesProtoporphyrinsQuality ControlReference ValuesTransferrin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1424108

Citation

Hastka, J, et al. "Washing Erythrocytes to Remove Interferents in Measurements of Zinc Protoporphyrin By Front-face Hematofluorometry." Clinical Chemistry, vol. 38, no. 11, 1992, pp. 2184-9.
Hastka J, Lasserre JJ, Schwarzbeck A, et al. Washing erythrocytes to remove interferents in measurements of zinc protoporphyrin by front-face hematofluorometry. Clin Chem. 1992;38(11):2184-9.
Hastka, J., Lasserre, J. J., Schwarzbeck, A., Strauch, M., & Hehlmann, R. (1992). Washing erythrocytes to remove interferents in measurements of zinc protoporphyrin by front-face hematofluorometry. Clinical Chemistry, 38(11), 2184-9.
Hastka J, et al. Washing Erythrocytes to Remove Interferents in Measurements of Zinc Protoporphyrin By Front-face Hematofluorometry. Clin Chem. 1992;38(11):2184-9. PubMed PMID: 1424108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Washing erythrocytes to remove interferents in measurements of zinc protoporphyrin by front-face hematofluorometry. AU - Hastka,J, AU - Lasserre,J J, AU - Schwarzbeck,A, AU - Strauch,M, AU - Hehlmann,R, PY - 1992/11/1/pubmed PY - 1992/11/1/medline PY - 1992/11/1/entrez SP - 2184 EP - 9 JF - Clinical chemistry JO - Clin Chem VL - 38 IS - 11 N2 - Zinc protoporphyrin (ZPP) is determined by hematofluorometry of whole blood to detect iron deficiency in blood donors. In hospitalized patients, ZPP did not correlate with established markers of iron status. We performed 4500 ZPP measurements with the Aviv front-face hematofluorometer in samples from 475 patients and measured ferritin, transferrin saturation, hemoglobin, and erythrocyte indices. We found that the fluorometric determination is affected by substances dissolved in plasma but that this interference can be eliminated by using washed erythrocytes. In validation tests the within-day variation was < 3.5%; the day-to-day variation was < 6.8%. In 130 healthy persons without iron deficiency, ZPP was < or = 40 mumol/mol heme, which we consider a normal value. Mean ZPP in 46 iron-deficient patients was 256 (SD 105) mumol/mol heme (correlation with ferritin: -0.73; with hemoglobin: -0.85; P < 0.001). When washed erythrocytes are used, the hematofluorometric determination of ZPP is sensitive and specific for detecting iron deficiency in otherwise healthy individuals and hospitalized patients. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/1424108/Washing_erythrocytes_to_remove_interferents_in_measurements_of_zinc_protoporphyrin_by_front_face_hematofluorometry_ DB - PRIME DP - Unbound Medicine ER -