Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast.Proc Natl Acad Sci U S A. 1992 Nov 15; 89(22):10887-91.PN
Abstract
A human cDNA encoding a galactokinase (EC 2.7.1.6) was isolated by complementation of a galactokinase-deficient (gal1-) strain of Saccharomyces cerevisiae. This cDNA encodes a predicted protein of 458 amino acids with 29% identity to galactokinase of Saccharomyces carlsbergensis. Previous studies have mapped a human galactokinase gene (GK1) to chromosome 17q23-25, closely linked to thymidine kinase. The galactokinase gene that we have isolated (GK2) is located on chromosome 15. The relationship between the disease locus for galactokinase deficiency galactosemia, which is responsible for cataracts in newborns and possibly presenile cataracts in adults, and the two galactokinase loci is unknown.
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Publisher Full Text
MeSH
Amino Acid SequenceAnimalsBacteriaBase SequenceChromosome BandingChromosome MappingChromosomes, Human, Pair 15Cloning, MolecularGalactokinaseGene LibraryGenetic Complementation TestHumansHybrid CellsMolecular Sequence DataOligodeoxyribonucleotidesOpen Reading FramesPhylogenyPolymerase Chain ReactionSaccharomycesSaccharomyces cerevisiaeSequence Homology, Amino Acid
Pub Type(s)
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Language
eng
PubMed ID
1438294
Citation
Lee, R T., et al. "Cloning of a Human Galactokinase Gene (GK2) On Chromosome 15 By Complementation in Yeast." Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 22, 1992, pp. 10887-91.
Lee RT, Peterson CL, Calman AF, et al. Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast. Proc Natl Acad Sci U S A. 1992;89(22):10887-91.
Lee, R. T., Peterson, C. L., Calman, A. F., Herskowitz, I., & O'Donnell, J. J. (1992). Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast. Proceedings of the National Academy of Sciences of the United States of America, 89(22), 10887-91.
Lee RT, et al. Cloning of a Human Galactokinase Gene (GK2) On Chromosome 15 By Complementation in Yeast. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10887-91. PubMed PMID: 1438294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast.
AU - Lee,R T,
AU - Peterson,C L,
AU - Calman,A F,
AU - Herskowitz,I,
AU - O'Donnell,J J,
PY - 1992/11/15/pubmed
PY - 1992/11/15/medline
PY - 1992/11/15/entrez
SP - 10887
EP - 91
JF - Proceedings of the National Academy of Sciences of the United States of America
JO - Proc Natl Acad Sci U S A
VL - 89
IS - 22
N2 - A human cDNA encoding a galactokinase (EC 2.7.1.6) was isolated by complementation of a galactokinase-deficient (gal1-) strain of Saccharomyces cerevisiae. This cDNA encodes a predicted protein of 458 amino acids with 29% identity to galactokinase of Saccharomyces carlsbergensis. Previous studies have mapped a human galactokinase gene (GK1) to chromosome 17q23-25, closely linked to thymidine kinase. The galactokinase gene that we have isolated (GK2) is located on chromosome 15. The relationship between the disease locus for galactokinase deficiency galactosemia, which is responsible for cataracts in newborns and possibly presenile cataracts in adults, and the two galactokinase loci is unknown.
SN - 0027-8424
UR - https://www.unboundmedicine.com/medline/citation/1438294/Cloning_of_a_human_galactokinase_gene__GK2__on_chromosome_15_by_complementation_in_yeast_
L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1438294
DB - PRIME
DP - Unbound Medicine
ER -