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Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast.
Proc Natl Acad Sci U S A. 1992 Nov 15; 89(22):10887-91.PN

Abstract

A human cDNA encoding a galactokinase (EC 2.7.1.6) was isolated by complementation of a galactokinase-deficient (gal1-) strain of Saccharomyces cerevisiae. This cDNA encodes a predicted protein of 458 amino acids with 29% identity to galactokinase of Saccharomyces carlsbergensis. Previous studies have mapped a human galactokinase gene (GK1) to chromosome 17q23-25, closely linked to thymidine kinase. The galactokinase gene that we have isolated (GK2) is located on chromosome 15. The relationship between the disease locus for galactokinase deficiency galactosemia, which is responsible for cataracts in newborns and possibly presenile cataracts in adults, and the two galactokinase loci is unknown.

Authors+Show Affiliations

Department of Ophthalmology, Kimura Laboratory of Clinical Investigation, San Francisco, CA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1438294

Citation

Lee, R T., et al. "Cloning of a Human Galactokinase Gene (GK2) On Chromosome 15 By Complementation in Yeast." Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 22, 1992, pp. 10887-91.
Lee RT, Peterson CL, Calman AF, et al. Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast. Proc Natl Acad Sci U S A. 1992;89(22):10887-91.
Lee, R. T., Peterson, C. L., Calman, A. F., Herskowitz, I., & O'Donnell, J. J. (1992). Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast. Proceedings of the National Academy of Sciences of the United States of America, 89(22), 10887-91.
Lee RT, et al. Cloning of a Human Galactokinase Gene (GK2) On Chromosome 15 By Complementation in Yeast. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10887-91. PubMed PMID: 1438294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast. AU - Lee,R T, AU - Peterson,C L, AU - Calman,A F, AU - Herskowitz,I, AU - O'Donnell,J J, PY - 1992/11/15/pubmed PY - 1992/11/15/medline PY - 1992/11/15/entrez SP - 10887 EP - 91 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 89 IS - 22 N2 - A human cDNA encoding a galactokinase (EC 2.7.1.6) was isolated by complementation of a galactokinase-deficient (gal1-) strain of Saccharomyces cerevisiae. This cDNA encodes a predicted protein of 458 amino acids with 29% identity to galactokinase of Saccharomyces carlsbergensis. Previous studies have mapped a human galactokinase gene (GK1) to chromosome 17q23-25, closely linked to thymidine kinase. The galactokinase gene that we have isolated (GK2) is located on chromosome 15. The relationship between the disease locus for galactokinase deficiency galactosemia, which is responsible for cataracts in newborns and possibly presenile cataracts in adults, and the two galactokinase loci is unknown. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/1438294/Cloning_of_a_human_galactokinase_gene__GK2__on_chromosome_15_by_complementation_in_yeast_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1438294 DB - PRIME DP - Unbound Medicine ER -