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Cocaine and regional brain monoamines in mice.
Pharmacol Biochem Behav. 1992 Oct; 43(2):395-403.PB

Abstract

Cocaine HCl (0, 10, or 50 mg/kg) was injected into adult male ICR mice IP. Thirty minutes later, brains were removed and nine regions were isolated: olfactory bulbs (OB), olfactory tubercles (OT), prefrontal cortex (PC), septum (SP), striatum (ST), amygdala (AMY), hypothalamus (HT), hippocampus (HC), and thalamus (TH). Using high-performance liquid chromatography, concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), and their major metabolites were determined. At 10 mg/kg cocaine, NE levels were increased in the AMY and its metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG), was decreased in the PC, AMY, and HT. DA levels were also increased in the AMY, while its intracellular metabolite, dihydroxyphenylacetic acid (DOPAC), was decreased in the ST and its extracellular metabolite, homovanillic acid (HVA), was decreased in the PC. 3-Methoxytyramine (3-MT) levels were not altered in any tissue. 5-HT levels were increased in the AMY, HT, and TH, while its metabolite 5-hydroxyindoleacetic acid (5-HIAA) was decreased in the OB and ST. MHPG/NE ratios were decreased in the PC, AMY, and HT as were those for HVA/DA. DOPAC/DA ratios were decreased in the ST and AMY and increased in the SP while those for 3-MT/DA were decreased in the TH and increased in the PC. 5-HIAA/5-HT ratios were decreased in the AMY, HC and TH. At 50 mg/kg cocaine, there was an increase in DA in the TH. There was a decrease in DOPAC, HVA, and 3-MT, as well as the DOPAC/DA ratio in the ST. In the OT, there was a decrease in DOPAC, the DOPAC/DA ratio, 3-MT, and the 3-MT/DA ratio.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Hadfield MG
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Milio C
No affiliation info available

MeSH

AnimalsBiogenic MonoaminesBrain ChemistryChromatography, High Pressure LiquidCocaineDopamineMaleMiceMice, Inbred ICRNorepinephrineSerotonin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1438479

Citation

Hadfield, M G., and C Milio. "Cocaine and Regional Brain Monoamines in Mice." Pharmacology, Biochemistry, and Behavior, vol. 43, no. 2, 1992, pp. 395-403.
Hadfield MG, Milio C. Cocaine and regional brain monoamines in mice. Pharmacol Biochem Behav. 1992;43(2):395-403.
Hadfield, M. G., & Milio, C. (1992). Cocaine and regional brain monoamines in mice. Pharmacology, Biochemistry, and Behavior, 43(2), 395-403.
Hadfield MG, Milio C. Cocaine and Regional Brain Monoamines in Mice. Pharmacol Biochem Behav. 1992;43(2):395-403. PubMed PMID: 1438479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cocaine and regional brain monoamines in mice. AU - Hadfield,M G, AU - Milio,C, PY - 1992/10/1/pubmed PY - 1992/10/1/medline PY - 1992/10/1/entrez SP - 395 EP - 403 JF - Pharmacology, biochemistry, and behavior JO - Pharmacol Biochem Behav VL - 43 IS - 2 N2 - Cocaine HCl (0, 10, or 50 mg/kg) was injected into adult male ICR mice IP. Thirty minutes later, brains were removed and nine regions were isolated: olfactory bulbs (OB), olfactory tubercles (OT), prefrontal cortex (PC), septum (SP), striatum (ST), amygdala (AMY), hypothalamus (HT), hippocampus (HC), and thalamus (TH). Using high-performance liquid chromatography, concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), and their major metabolites were determined. At 10 mg/kg cocaine, NE levels were increased in the AMY and its metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG), was decreased in the PC, AMY, and HT. DA levels were also increased in the AMY, while its intracellular metabolite, dihydroxyphenylacetic acid (DOPAC), was decreased in the ST and its extracellular metabolite, homovanillic acid (HVA), was decreased in the PC. 3-Methoxytyramine (3-MT) levels were not altered in any tissue. 5-HT levels were increased in the AMY, HT, and TH, while its metabolite 5-hydroxyindoleacetic acid (5-HIAA) was decreased in the OB and ST. MHPG/NE ratios were decreased in the PC, AMY, and HT as were those for HVA/DA. DOPAC/DA ratios were decreased in the ST and AMY and increased in the SP while those for 3-MT/DA were decreased in the TH and increased in the PC. 5-HIAA/5-HT ratios were decreased in the AMY, HC and TH. At 50 mg/kg cocaine, there was an increase in DA in the TH. There was a decrease in DOPAC, HVA, and 3-MT, as well as the DOPAC/DA ratio in the ST. In the OT, there was a decrease in DOPAC, the DOPAC/DA ratio, 3-MT, and the 3-MT/DA ratio.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0091-3057 UR - https://www.unboundmedicine.com/medline/citation/1438479/Cocaine_and_regional_brain_monoamines_in_mice_ DB - PRIME DP - Unbound Medicine ER -