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Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937.
J Bacteriol. 1992 Dec; 174(23):7807-18.JB

Abstract

To depolymerize plant pectin, the phytopathogenic enterobacterium Erwinia chrysanthemi produces a series of enzymes which include a pectin-methyl-esterase encoded by the pem gene and five isoenzymes of pectate lyases encoded by the five genes pelA, pelB, pelC, pelD, and pelE. We have constructed transcriptional fusions between the pectinase gene promoters and the uidA gene, encoding beta-glucuronidase, to study the regulation of these E. chrysanthemi pectinase genes individually. The transcription of the pectinase genes is dependent on many environmental conditions. All the fusions were induced by pectic catabolic products and responded, to different degrees, to growth phase, catabolite repression, temperature, and nitrogen starvation. Transcription of pelA, pelD, and pelE was also increased in anaerobic growth conditions. High osmolarity of the culture medium increased expression of pelE but decreased that of pelD; the other pectinase genes were not affected. The level of expression of each gene was different. Transcription of pelA was very low under all growth conditions. The expression of the pelB, pelC, and pem genes was intermediate. The pelE gene had a high basal level of expression. Expression of pelD was generally the most affected by changes in culture conditions and showed a low basal level but very high induced levels. These differences in the expression of the pectinase genes of E. chrysanthemi 3937 presumably reflect their role during infection of plants, because the degradation of pectic polymers of the plant cell walls is the main determinant of tissue maceration caused by soft rot erwiniae.

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Authors+Show Affiliations

Hugouvieux-Cotte-Pattat N
Laboratoire de Génétique Moléculaire des Microorganismes, URA-CNRS, Institut National des Sciences Appliquées batiment 406, Villeurbanne, France.
Dominguez H
No affiliation info available
Robert-Baudouy J
No affiliation info available

MeSH

AnaerobiosisBiodegradation, EnvironmentalCarbohydrate SequenceCarboxylic Ester HydrolasesEnzyme RepressionErwiniaGene Expression Regulation, BacterialGenes, BacterialGlucuronidaseMolecular Sequence DataNitrogenOsmolar ConcentrationPectinsPolygalacturonasePolysaccharide-LyasesRecombinant Fusion ProteinsRepressor ProteinsTemperatureTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1447147

Citation

Hugouvieux-Cotte-Pattat, N, et al. "Environmental Conditions Affect Transcription of the Pectinase Genes of Erwinia Chrysanthemi 3937." Journal of Bacteriology, vol. 174, no. 23, 1992, pp. 7807-18.
Hugouvieux-Cotte-Pattat N, Dominguez H, Robert-Baudouy J. Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937. J Bacteriol. 1992;174(23):7807-18.
Hugouvieux-Cotte-Pattat, N., Dominguez, H., & Robert-Baudouy, J. (1992). Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937. Journal of Bacteriology, 174(23), 7807-18.
Hugouvieux-Cotte-Pattat N, Dominguez H, Robert-Baudouy J. Environmental Conditions Affect Transcription of the Pectinase Genes of Erwinia Chrysanthemi 3937. J Bacteriol. 1992;174(23):7807-18. PubMed PMID: 1447147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937. AU - Hugouvieux-Cotte-Pattat,N, AU - Dominguez,H, AU - Robert-Baudouy,J, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 7807 EP - 18 JF - Journal of bacteriology JO - J Bacteriol VL - 174 IS - 23 N2 - To depolymerize plant pectin, the phytopathogenic enterobacterium Erwinia chrysanthemi produces a series of enzymes which include a pectin-methyl-esterase encoded by the pem gene and five isoenzymes of pectate lyases encoded by the five genes pelA, pelB, pelC, pelD, and pelE. We have constructed transcriptional fusions between the pectinase gene promoters and the uidA gene, encoding beta-glucuronidase, to study the regulation of these E. chrysanthemi pectinase genes individually. The transcription of the pectinase genes is dependent on many environmental conditions. All the fusions were induced by pectic catabolic products and responded, to different degrees, to growth phase, catabolite repression, temperature, and nitrogen starvation. Transcription of pelA, pelD, and pelE was also increased in anaerobic growth conditions. High osmolarity of the culture medium increased expression of pelE but decreased that of pelD; the other pectinase genes were not affected. The level of expression of each gene was different. Transcription of pelA was very low under all growth conditions. The expression of the pelB, pelC, and pem genes was intermediate. The pelE gene had a high basal level of expression. Expression of pelD was generally the most affected by changes in culture conditions and showed a low basal level but very high induced levels. These differences in the expression of the pectinase genes of E. chrysanthemi 3937 presumably reflect their role during infection of plants, because the degradation of pectic polymers of the plant cell walls is the main determinant of tissue maceration caused by soft rot erwiniae. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/1447147/Environmental_conditions_affect_transcription_of_the_pectinase_genes_of_Erwinia_chrysanthemi_3937_ DB - PRIME DP - Unbound Medicine ER -