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Role of insulin-like growth factor binding protein (IGFBP)-3 in TGF-beta- and GDF-8 (myostatin)-induced suppression of proliferation in porcine embryonic myogenic cell cultures.
J Cell Physiol. 2003 Nov; 197(2):225-31.JC

Abstract

Both transforming growth factor (TGF-beta) and growth and development factor (GDF)-8 (myostatin) affect muscle differentiation by suppressing proliferation and differentiation of myogenic cells. In contrast, insulin-like growth factors (IGFs) stimulate both proliferation and differentiation of myogenic cells. In vivo, IGFs are found in association with a family of high-affinity insulin-like growth factor binding proteins (IGFBP 1-6) that affect their biological activity. Treatment of porcine embryonic myogenic cell (PEMC) cultures with either TGF-beta(1) or GDF-8 suppressed proliferation and increased production of IGFBP-3 protein and mRNA (P < 0.005). An anti-IGFBP-3 antibody that neutralizes the biological activity of IGFBP-3 reduced the ability of either TGF-beta(1) or GDF-8 to suppress PEMC proliferation (P < 0.005). However, this antibody did not affect proliferation rate in the presence of both TGF-beta(1) and GDF-8. These data show that IGFBP-3 plays a role in mediating the activity of either TGF-beta(1) or GDF-8 alone but not when both TGF-beta(1) and GDF-8 are present. In contrast to findings in T47D breast cancer cells, treatment of PEMC cultures with IGFBP-3 did not result in increased levels of phosphosmad-2. Since TGF-beta and GDF-8 are believed to play a significant role in regulating proliferation and differentiation of myogenic cells, our current data showing that IGFBP-3 plays a role in mediating the activity of these growth factors in muscle cell cultures strongly suggest that IGFBP-3 also may be involved in regulating these processes in myogenic cells.

Authors+Show Affiliations

Department of Animal Science, University of Minnesota, St. Paul, Minnesota, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

14502562

Citation

Kamanga-Sollo, E, et al. "Role of Insulin-like Growth Factor Binding Protein (IGFBP)-3 in TGF-beta- and GDF-8 (myostatin)-induced Suppression of Proliferation in Porcine Embryonic Myogenic Cell Cultures." Journal of Cellular Physiology, vol. 197, no. 2, 2003, pp. 225-31.
Kamanga-Sollo E, Pampusch MS, White ME, et al. Role of insulin-like growth factor binding protein (IGFBP)-3 in TGF-beta- and GDF-8 (myostatin)-induced suppression of proliferation in porcine embryonic myogenic cell cultures. J Cell Physiol. 2003;197(2):225-31.
Kamanga-Sollo, E., Pampusch, M. S., White, M. E., & Dayton, W. R. (2003). Role of insulin-like growth factor binding protein (IGFBP)-3 in TGF-beta- and GDF-8 (myostatin)-induced suppression of proliferation in porcine embryonic myogenic cell cultures. Journal of Cellular Physiology, 197(2), 225-31.
Kamanga-Sollo E, et al. Role of Insulin-like Growth Factor Binding Protein (IGFBP)-3 in TGF-beta- and GDF-8 (myostatin)-induced Suppression of Proliferation in Porcine Embryonic Myogenic Cell Cultures. J Cell Physiol. 2003;197(2):225-31. PubMed PMID: 14502562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of insulin-like growth factor binding protein (IGFBP)-3 in TGF-beta- and GDF-8 (myostatin)-induced suppression of proliferation in porcine embryonic myogenic cell cultures. AU - Kamanga-Sollo,E, AU - Pampusch,M S, AU - White,M E, AU - Dayton,W R, PY - 2003/9/23/pubmed PY - 2003/11/11/medline PY - 2003/9/23/entrez SP - 225 EP - 31 JF - Journal of cellular physiology JO - J Cell Physiol VL - 197 IS - 2 N2 - Both transforming growth factor (TGF-beta) and growth and development factor (GDF)-8 (myostatin) affect muscle differentiation by suppressing proliferation and differentiation of myogenic cells. In contrast, insulin-like growth factors (IGFs) stimulate both proliferation and differentiation of myogenic cells. In vivo, IGFs are found in association with a family of high-affinity insulin-like growth factor binding proteins (IGFBP 1-6) that affect their biological activity. Treatment of porcine embryonic myogenic cell (PEMC) cultures with either TGF-beta(1) or GDF-8 suppressed proliferation and increased production of IGFBP-3 protein and mRNA (P < 0.005). An anti-IGFBP-3 antibody that neutralizes the biological activity of IGFBP-3 reduced the ability of either TGF-beta(1) or GDF-8 to suppress PEMC proliferation (P < 0.005). However, this antibody did not affect proliferation rate in the presence of both TGF-beta(1) and GDF-8. These data show that IGFBP-3 plays a role in mediating the activity of either TGF-beta(1) or GDF-8 alone but not when both TGF-beta(1) and GDF-8 are present. In contrast to findings in T47D breast cancer cells, treatment of PEMC cultures with IGFBP-3 did not result in increased levels of phosphosmad-2. Since TGF-beta and GDF-8 are believed to play a significant role in regulating proliferation and differentiation of myogenic cells, our current data showing that IGFBP-3 plays a role in mediating the activity of these growth factors in muscle cell cultures strongly suggest that IGFBP-3 also may be involved in regulating these processes in myogenic cells. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/14502562/Role_of_insulin_like_growth_factor_binding_protein__IGFBP__3_in_TGF_beta__and_GDF_8__myostatin__induced_suppression_of_proliferation_in_porcine_embryonic_myogenic_cell_cultures_ L2 - https://doi.org/10.1002/jcp.10362 DB - PRIME DP - Unbound Medicine ER -