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Leptin messenger ribonucleic acid (mRNA) content in the human placenta at term: relationship to levels of leptin in cord blood and placental weight.
Gynecol Endocrinol 2003; 17(4):311-6GE

Abstract

During fetal life, placental tissue represents an additional source of leptin for the mother and conceptus. It has been suggested that feto-placental production of leptin may be involved in placental and fetal growth regulation. The aim of this study was to examine the correlation between leptin mRNA expression in the placenta and the concentrations of leptin in cord blood. A total of 30 healthy, pregnant women who gave birth to healthy neonates were included in the study. Maternal blood (obtained from the cubital vein) and umbilical cord blood were drawn immediately after birth. Serum leptin concentration was determined by enzyme-linked immunosorbent assay and serum insulin concentration was measured by radioimmunoassay. Leptin mRNA was measured in placental tissue by a reverse transcriptase-polymerase chain reaction. The estimated mean leptin mRNA expression in placenta was 4.65 +/- 1.83 pg mRNA/microg DNA. Leptin mRNA correlated with cord serum leptin concentrations (r = 0.3691, p = 0.045). Placental weight correlated with placental leptin mRNA (r = 0.3686, p = 0.045). The mean leptin concentration in cord serum at birth was slightly lower (3.1 +/- 1.9 ng/ml) than that found in maternal serum (3.9 +/- 1.2 ng/ml). A positive correlation was observed between cord and maternal serum leptin levels (r = 0.58, p = 0.001). The mean insulin concentration in maternal serum was not significantly higher than that in umbilical serum: 22.2 +/- 17.8 microIU/ml vs. 6.9 +/- 3.6 microIU/ml; r = 0.069, p = 0.71). Neither maternal nor umbilical insulin concentrations correlated with leptin concentration in cord or maternal peripheral serum.

Authors+Show Affiliations

Second Department of Gynecology, University School of Medicine, ul. Jaczewskiego 8, 20-090 Lublin, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14503975

Citation

Jakimiuk, A J., et al. "Leptin Messenger Ribonucleic Acid (mRNA) Content in the Human Placenta at Term: Relationship to Levels of Leptin in Cord Blood and Placental Weight." Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology, vol. 17, no. 4, 2003, pp. 311-6.
Jakimiuk AJ, Skałba P, Huterski D, et al. Leptin messenger ribonucleic acid (mRNA) content in the human placenta at term: relationship to levels of leptin in cord blood and placental weight. Gynecol Endocrinol. 2003;17(4):311-6.
Jakimiuk, A. J., Skałba, P., Huterski, D., Tarkowski, R., Haczyński, J., & Magoffin, D. A. (2003). Leptin messenger ribonucleic acid (mRNA) content in the human placenta at term: relationship to levels of leptin in cord blood and placental weight. Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology, 17(4), pp. 311-6.
Jakimiuk AJ, et al. Leptin Messenger Ribonucleic Acid (mRNA) Content in the Human Placenta at Term: Relationship to Levels of Leptin in Cord Blood and Placental Weight. Gynecol Endocrinol. 2003;17(4):311-6. PubMed PMID: 14503975.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leptin messenger ribonucleic acid (mRNA) content in the human placenta at term: relationship to levels of leptin in cord blood and placental weight. AU - Jakimiuk,A J, AU - Skałba,P, AU - Huterski,D, AU - Tarkowski,R, AU - Haczyński,J, AU - Magoffin,D A, PY - 2003/9/25/pubmed PY - 2004/5/7/medline PY - 2003/9/25/entrez SP - 311 EP - 6 JF - Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology JO - Gynecol. Endocrinol. VL - 17 IS - 4 N2 - During fetal life, placental tissue represents an additional source of leptin for the mother and conceptus. It has been suggested that feto-placental production of leptin may be involved in placental and fetal growth regulation. The aim of this study was to examine the correlation between leptin mRNA expression in the placenta and the concentrations of leptin in cord blood. A total of 30 healthy, pregnant women who gave birth to healthy neonates were included in the study. Maternal blood (obtained from the cubital vein) and umbilical cord blood were drawn immediately after birth. Serum leptin concentration was determined by enzyme-linked immunosorbent assay and serum insulin concentration was measured by radioimmunoassay. Leptin mRNA was measured in placental tissue by a reverse transcriptase-polymerase chain reaction. The estimated mean leptin mRNA expression in placenta was 4.65 +/- 1.83 pg mRNA/microg DNA. Leptin mRNA correlated with cord serum leptin concentrations (r = 0.3691, p = 0.045). Placental weight correlated with placental leptin mRNA (r = 0.3686, p = 0.045). The mean leptin concentration in cord serum at birth was slightly lower (3.1 +/- 1.9 ng/ml) than that found in maternal serum (3.9 +/- 1.2 ng/ml). A positive correlation was observed between cord and maternal serum leptin levels (r = 0.58, p = 0.001). The mean insulin concentration in maternal serum was not significantly higher than that in umbilical serum: 22.2 +/- 17.8 microIU/ml vs. 6.9 +/- 3.6 microIU/ml; r = 0.069, p = 0.71). Neither maternal nor umbilical insulin concentrations correlated with leptin concentration in cord or maternal peripheral serum. SN - 0951-3590 UR - https://www.unboundmedicine.com/medline/citation/14503975/Leptin_messenger_ribonucleic_acid__mRNA__content_in_the_human_placenta_at_term:_relationship_to_levels_of_leptin_in_cord_blood_and_placental_weight_ DB - PRIME DP - Unbound Medicine ER -