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Glucose-insulin kinetics of the extravascular bioartificial pancreas. A study using microencapsulated rat islets.
ASAIO J. 1992 Oct-Dec; 38(4):851-4.AJ

Abstract

The success of the extravascular bioartificial pancreas (BAP) is contingent on the rapid transfer of the glycemic signal across both an extravascular compartment and a semipermeable membrane and of insulin from the BAP to the recipient. To examine the possibility of microencapsulated islets such as the BAP to achieve satisfactory in vivo glucose-insulin kinetics, islets were isolated from Lewis rats, encapsulated in poly-L-lysine-alginate membranes, and isogenically transplanted into the peritoneal cavity of 14 streptozotocin induced diabetic rats. Fasting blood glucose (BG) was measured and intravenous glucose tolerance was tested at 8-10 weeks and compared with three control groups: 1) normal Lewis rats (n = 6); 2) untreated diabetic rats (n = 5); and 3) diabetic rats that received intraperitoneal implants of empty capsules (n = 4). Ten animals that received microencapsulated islets (5,271 +/- 431) promptly became normoglycemic, with a mean BG of 128 +/- 17 ng/dl 3 days after transplantation and maintained this level > 100 days. Intravenous glucose tolerance K-value for the group was 3.84 +/- 0.32 compared with 3.96 +/- 0.39 (p = 0.83) for the normal control group, and 0.60 +/- 0.12 (p < 0.01) and 0.40 +/- 0.15 (p < 0.01) for the diabetic control groups with and without empty capsules. The authors conclude from these results that, given sufficient beta-cell mass, a BAP without any vascular access can respond appropriately to an increase in blood glucose concentration, without overshoot hypoglycemia and within a lag lapse compatible with normal physiologic insulin delivery.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Islet Transplant Center, VA Wadsworth Medical Center, Los Angeles, California 90073.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1450485

Citation

Soon-Shiong, P, et al. "Glucose-insulin Kinetics of the Extravascular Bioartificial Pancreas. a Study Using Microencapsulated Rat Islets." ASAIO Journal (American Society for Artificial Internal Organs : 1992), vol. 38, no. 4, 1992, pp. 851-4.
Soon-Shiong P, Heintz R, Yao Z, et al. Glucose-insulin kinetics of the extravascular bioartificial pancreas. A study using microencapsulated rat islets. ASAIO J. 1992;38(4):851-4.
Soon-Shiong, P., Heintz, R., Yao, Z., Yao, Q., Sanford, P., Lanza, R. P., & Meredith, N. (1992). Glucose-insulin kinetics of the extravascular bioartificial pancreas. A study using microencapsulated rat islets. ASAIO Journal (American Society for Artificial Internal Organs : 1992), 38(4), 851-4.
Soon-Shiong P, et al. Glucose-insulin Kinetics of the Extravascular Bioartificial Pancreas. a Study Using Microencapsulated Rat Islets. ASAIO J. 1992 Oct-Dec;38(4):851-4. PubMed PMID: 1450485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucose-insulin kinetics of the extravascular bioartificial pancreas. A study using microencapsulated rat islets. AU - Soon-Shiong,P, AU - Heintz,R, AU - Yao,Z, AU - Yao,Q, AU - Sanford,P, AU - Lanza,R P, AU - Meredith,N, PY - 1992/10/1/pubmed PY - 1992/10/1/medline PY - 1992/10/1/entrez SP - 851 EP - 4 JF - ASAIO journal (American Society for Artificial Internal Organs : 1992) JO - ASAIO J VL - 38 IS - 4 N2 - The success of the extravascular bioartificial pancreas (BAP) is contingent on the rapid transfer of the glycemic signal across both an extravascular compartment and a semipermeable membrane and of insulin from the BAP to the recipient. To examine the possibility of microencapsulated islets such as the BAP to achieve satisfactory in vivo glucose-insulin kinetics, islets were isolated from Lewis rats, encapsulated in poly-L-lysine-alginate membranes, and isogenically transplanted into the peritoneal cavity of 14 streptozotocin induced diabetic rats. Fasting blood glucose (BG) was measured and intravenous glucose tolerance was tested at 8-10 weeks and compared with three control groups: 1) normal Lewis rats (n = 6); 2) untreated diabetic rats (n = 5); and 3) diabetic rats that received intraperitoneal implants of empty capsules (n = 4). Ten animals that received microencapsulated islets (5,271 +/- 431) promptly became normoglycemic, with a mean BG of 128 +/- 17 ng/dl 3 days after transplantation and maintained this level > 100 days. Intravenous glucose tolerance K-value for the group was 3.84 +/- 0.32 compared with 3.96 +/- 0.39 (p = 0.83) for the normal control group, and 0.60 +/- 0.12 (p < 0.01) and 0.40 +/- 0.15 (p < 0.01) for the diabetic control groups with and without empty capsules. The authors conclude from these results that, given sufficient beta-cell mass, a BAP without any vascular access can respond appropriately to an increase in blood glucose concentration, without overshoot hypoglycemia and within a lag lapse compatible with normal physiologic insulin delivery.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 1058-2916 UR - https://www.unboundmedicine.com/medline/citation/1450485/Glucose_insulin_kinetics_of_the_extravascular_bioartificial_pancreas__A_study_using_microencapsulated_rat_islets_ L2 - https://journals.lww.com/1450485.pmid DB - PRIME DP - Unbound Medicine ER -