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Gonadotropin-releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis.
Fertil Steril. 2003 Sep; 80 Suppl 2:702-7.FS

Abstract

OBJECTIVE

There is growing evidence that suggests a direct action of gonadotropin-releasing hormone agonist (GnRH-a) on endometrial growth. Consequently, our purpose was to evaluate the effect of GnRH-a on in vitro eutopic endometrial cell growth and apoptosis.

DESIGN

Prospective study.

SETTING

Research institute and clinical fertility center.

PATIENT(S)

Sixteen women with untreated endometriosis and 14 controls.

INTERVENTION(S)

Biopsy specimens of eutopic endometrium were obtained from all subjects. Apoptosis and cell proliferation were examined in epithelial endometrial cell cultures after incubation with leuprolide acetate (LA), antide, and a combination of both.

MAIN OUTCOME MEASURE(S)

The percentage of apoptotic cells was evaluated by the acridine orange-ethidium bromide technique; cell proliferation was assessed by (3)H-thymidine incorporation.

RESULT(S)

Leuprolide acetate (LA) (100 ng/mL) enhanced apoptosis in endometrial cultures from patients with endometriosis and controls, and this effect was reversed by antide 10(-7)M. Cell proliferation was down-regulated by LA at 1, 10, and 100 ng/mL in cultures from women without and with endometriosis. The addition of antide 10(-7)M reversed this inhibition.

CONCLUSION(S)

GnRH-a appears to have a direct effect by enhancing the apoptotic index and decreasing the cell proliferation in endometrial cells.

Authors+Show Affiliations

Instituto de Biología y Medicina Experimental; Department of Gynecology, Buenos Aires, Argentina. meresman@dna.uba.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14505742

Citation

Meresman, Gabriela F., et al. "Gonadotropin-releasing Hormone Agonist Induces Apoptosis and Reduces Cell Proliferation in Eutopic Endometrial Cultures From Women With Endometriosis." Fertility and Sterility, vol. 80 Suppl 2, 2003, pp. 702-7.
Meresman GF, Bilotas M, Buquet RA, et al. Gonadotropin-releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis. Fertil Steril. 2003;80 Suppl 2:702-7.
Meresman, G. F., Bilotas, M., Buquet, R. A., Barañao, R. I., Sueldo, C., & Tesone, M. (2003). Gonadotropin-releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis. Fertility and Sterility, 80 Suppl 2, 702-7.
Meresman GF, et al. Gonadotropin-releasing Hormone Agonist Induces Apoptosis and Reduces Cell Proliferation in Eutopic Endometrial Cultures From Women With Endometriosis. Fertil Steril. 2003;80 Suppl 2:702-7. PubMed PMID: 14505742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gonadotropin-releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis. AU - Meresman,Gabriela F, AU - Bilotas,Mariela, AU - Buquet,Ricardo A, AU - Barañao,Rosa I, AU - Sueldo,Carlos, AU - Tesone,Marta, PY - 2003/9/25/pubmed PY - 2003/11/5/medline PY - 2003/9/25/entrez SP - 702 EP - 7 JF - Fertility and sterility JO - Fertil Steril VL - 80 Suppl 2 N2 - OBJECTIVE: There is growing evidence that suggests a direct action of gonadotropin-releasing hormone agonist (GnRH-a) on endometrial growth. Consequently, our purpose was to evaluate the effect of GnRH-a on in vitro eutopic endometrial cell growth and apoptosis. DESIGN: Prospective study. SETTING: Research institute and clinical fertility center. PATIENT(S): Sixteen women with untreated endometriosis and 14 controls. INTERVENTION(S): Biopsy specimens of eutopic endometrium were obtained from all subjects. Apoptosis and cell proliferation were examined in epithelial endometrial cell cultures after incubation with leuprolide acetate (LA), antide, and a combination of both. MAIN OUTCOME MEASURE(S): The percentage of apoptotic cells was evaluated by the acridine orange-ethidium bromide technique; cell proliferation was assessed by (3)H-thymidine incorporation. RESULT(S): Leuprolide acetate (LA) (100 ng/mL) enhanced apoptosis in endometrial cultures from patients with endometriosis and controls, and this effect was reversed by antide 10(-7)M. Cell proliferation was down-regulated by LA at 1, 10, and 100 ng/mL in cultures from women without and with endometriosis. The addition of antide 10(-7)M reversed this inhibition. CONCLUSION(S): GnRH-a appears to have a direct effect by enhancing the apoptotic index and decreasing the cell proliferation in endometrial cells. SN - 0015-0282 UR - https://www.unboundmedicine.com/medline/citation/14505742/Gonadotropin_releasing_hormone_agonist_induces_apoptosis_and_reduces_cell_proliferation_in_eutopic_endometrial_cultures_from_women_with_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015028203007696 DB - PRIME DP - Unbound Medicine ER -