Tags

Type your tag names separated by a space and hit enter

Pancreatic carcinoma in carriers of a specific 19 base pair deletion of CDKN2A/p16 (p16-leiden).
Clin Cancer Res 2003; 9(10 Pt 1):3598-605CC

Abstract

PURPOSE

The purpose is to document the clinical, pathological, and genetic features of pancreatic carcinoma (PC) in carriers of a specific p16-Leiden mutation (a 19-bp deletion in exon 2 of the CDKN2A gene).

EXPERIMENTAL DESIGN

Clinical data and paraffin embedded tissue were obtained from 12 patients of p16-Leiden-positive families with PC. Because of the known 19-bp germ-line deletion, we could specifically analyze the genotype of the wild-type allele for loss of heterozygosity. K-ras codon 12 mutations were determined and immunohistochemical testing for p16, Tp53, Smad4, and cyclooxygenase 2 was performed.

RESULTS

The average age of subjects that developed PC (8 males) was 58 years (range, 43-74 years). Histology was considered as conventional ductal adenocarcinoma in 11 of 12 and neuroendocrine carcinoma (1 of 12). The carcinomas were located in the head (10 of 12), corpus (1 of 12), and tail (1 of 12) of the pancreas. The specific p16-Leiden mutation was confirmed in the tissue of all subjects. Loss of heterozygosity of the wild-type allele was present in 2 of 7 tumors analyzed. Immunostaining for p16 was negative in 10 of 10. Tp53 mutations were detected in 5 of 12. Smad4 was negative in 5 of 12 and cyclooxygenase 2 was overexpressed in 11 of 12. K-ras codon 12 mutations were present in 9 of 10 and in three precursor lesions even before abrogation of p16 protein expression was seen (one of three).

CONCLUSIONS

The p16-Leiden deletion was associated with progression toward conventional ductal adenocarcinomas in all cases but one. Our observations might support the feasibility of early diagnosis of PC in p16-Leiden mutation carriers and might also indicate that chemoprevention needs consideration.

Authors+Show Affiliations

Departments of Gastroenterology, Leiden University Medical Center, Leiden PB 9600, the Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14506146

Citation

de vos tot Nederveen Cappel, Wouter H., et al. "Pancreatic Carcinoma in Carriers of a Specific 19 Base Pair Deletion of CDKN2A/p16 (p16-leiden)." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 9, no. 10 Pt 1, 2003, pp. 3598-605.
de vos tot Nederveen Cappel WH, Offerhaus GJ, van Puijenbroek M, et al. Pancreatic carcinoma in carriers of a specific 19 base pair deletion of CDKN2A/p16 (p16-leiden). Clin Cancer Res. 2003;9(10 Pt 1):3598-605.
de vos tot Nederveen Cappel, W. H., Offerhaus, G. J., van Puijenbroek, M., Caspers, E., Gruis, N. A., De Snoo, F. A., ... Morreau, H. (2003). Pancreatic carcinoma in carriers of a specific 19 base pair deletion of CDKN2A/p16 (p16-leiden). Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 9(10 Pt 1), pp. 3598-605.
de vos tot Nederveen Cappel WH, et al. Pancreatic Carcinoma in Carriers of a Specific 19 Base Pair Deletion of CDKN2A/p16 (p16-leiden). Clin Cancer Res. 2003 Sep 1;9(10 Pt 1):3598-605. PubMed PMID: 14506146.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pancreatic carcinoma in carriers of a specific 19 base pair deletion of CDKN2A/p16 (p16-leiden). AU - de vos tot Nederveen Cappel,Wouter H, AU - Offerhaus,G Johan A, AU - van Puijenbroek,Marjo, AU - Caspers,Eric, AU - Gruis,Nelleke A, AU - De Snoo,Femke A, AU - Lamers,Cornelis B H W, AU - Griffioen,Gerrit, AU - Bergman,Wilma, AU - Vasen,Hans F A, AU - Morreau,Hans, PY - 2003/9/25/pubmed PY - 2004/5/29/medline PY - 2003/9/25/entrez SP - 3598 EP - 605 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin. Cancer Res. VL - 9 IS - 10 Pt 1 N2 - PURPOSE: The purpose is to document the clinical, pathological, and genetic features of pancreatic carcinoma (PC) in carriers of a specific p16-Leiden mutation (a 19-bp deletion in exon 2 of the CDKN2A gene). EXPERIMENTAL DESIGN: Clinical data and paraffin embedded tissue were obtained from 12 patients of p16-Leiden-positive families with PC. Because of the known 19-bp germ-line deletion, we could specifically analyze the genotype of the wild-type allele for loss of heterozygosity. K-ras codon 12 mutations were determined and immunohistochemical testing for p16, Tp53, Smad4, and cyclooxygenase 2 was performed. RESULTS: The average age of subjects that developed PC (8 males) was 58 years (range, 43-74 years). Histology was considered as conventional ductal adenocarcinoma in 11 of 12 and neuroendocrine carcinoma (1 of 12). The carcinomas were located in the head (10 of 12), corpus (1 of 12), and tail (1 of 12) of the pancreas. The specific p16-Leiden mutation was confirmed in the tissue of all subjects. Loss of heterozygosity of the wild-type allele was present in 2 of 7 tumors analyzed. Immunostaining for p16 was negative in 10 of 10. Tp53 mutations were detected in 5 of 12. Smad4 was negative in 5 of 12 and cyclooxygenase 2 was overexpressed in 11 of 12. K-ras codon 12 mutations were present in 9 of 10 and in three precursor lesions even before abrogation of p16 protein expression was seen (one of three). CONCLUSIONS: The p16-Leiden deletion was associated with progression toward conventional ductal adenocarcinomas in all cases but one. Our observations might support the feasibility of early diagnosis of PC in p16-Leiden mutation carriers and might also indicate that chemoprevention needs consideration. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/14506146/Pancreatic_carcinoma_in_carriers_of_a_specific_19_base_pair_deletion_of_CDKN2A/p16__p16_leiden__ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=14506146 DB - PRIME DP - Unbound Medicine ER -