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Possible modulators of IL-8 and GRO-alpha production by granulosa cells.
Am J Reprod Immunol. 2003 Jul; 50(1):98-103.AJ

Abstract

PROBLEM

Interleukin-8 (IL-8) and growth-regulated oncogene-alpha (GRO-alpha) have been proved to be important modulators of leukocyte chemotaxis in the mechanism of human ovulation. This study investigated the possible effects of IL-1alpha, tumor necrosis factor-alpha (TNF-alpha), protein kinase C (PKC) activators (TPA), and db-cyclic adenosine monophosphate (cAMP) on IL-8 and GRO-alpha production by immortalized GC1a and granulosa-lutein cells.

METHOD OF STUDY

Confluent granulosa-lutein cells were placed in serum-free medium before incubated for 8 hr with the above-mentioned test agents. Finally, we measured IL-8 and GRO-alpha levels in the culture media using an enzyme-linked immunosorbent assay (ELISA).

RESULTS

Treatment of granulosa-lutein cells with of IL-1alpha (1 nM), TNF-alpha (1 nM), TPA (1 nM) and db-cAMP (100 microM) produced higher levels of IL-8 than untreated cells by 8 hr (2274.7 +/- 146.3, 1489.8 +/- 190.1, 1452.9 +/- 152.7, 1313.6 +/- 48.4 pg/mL, respectively; control = 457.7 +/- 38.2 pg/mL; P < 0.001). Treatment of granulosa-lutein cells with 1 nM of IL-1alpha, TNFalpha, TPA, and db-cAMP (100 microM) resulted in higher levels of GRO-alpha than untreated cells by 8 hr (993.7 +/- 9.5, 171.4 +/- 6.5, 147.5 +/- 6.7, 472.4 +/- 16.2 pg/mL respectively; control = 73.8 +/- 8.2 pg/mL; P < 0.001).

CONCLUSIONS

Our data strongly suggests roles for IL-1alpha, TNFalpha, and PKC activators in the inflammation-like mechanism of human ovulation. Furthermore, our study suggests a positive, but still debatable, role for cAMP in the same mechanism.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Oita Medical University, Hasama, Oita, Japan. kosayz@yahoo.ieNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14506934

Citation

Zeineh, Kosay, et al. "Possible Modulators of IL-8 and GRO-alpha Production By Granulosa Cells." American Journal of Reproductive Immunology (New York, N.Y. : 1989), vol. 50, no. 1, 2003, pp. 98-103.
Zeineh K, Kawano Y, Fukuda J, et al. Possible modulators of IL-8 and GRO-alpha production by granulosa cells. Am J Reprod Immunol. 2003;50(1):98-103.
Zeineh, K., Kawano, Y., Fukuda, J., Nasu, K., Narahara, H., & Miyakawa, I. (2003). Possible modulators of IL-8 and GRO-alpha production by granulosa cells. American Journal of Reproductive Immunology (New York, N.Y. : 1989), 50(1), 98-103.
Zeineh K, et al. Possible Modulators of IL-8 and GRO-alpha Production By Granulosa Cells. Am J Reprod Immunol. 2003;50(1):98-103. PubMed PMID: 14506934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible modulators of IL-8 and GRO-alpha production by granulosa cells. AU - Zeineh,Kosay, AU - Kawano,Yasushi, AU - Fukuda,Junichiro, AU - Nasu,Kaei, AU - Narahara,Hisashi, AU - Miyakawa,Isao, PY - 2003/9/26/pubmed PY - 2004/3/10/medline PY - 2003/9/26/entrez SP - 98 EP - 103 JF - American journal of reproductive immunology (New York, N.Y. : 1989) JO - Am J Reprod Immunol VL - 50 IS - 1 N2 - PROBLEM: Interleukin-8 (IL-8) and growth-regulated oncogene-alpha (GRO-alpha) have been proved to be important modulators of leukocyte chemotaxis in the mechanism of human ovulation. This study investigated the possible effects of IL-1alpha, tumor necrosis factor-alpha (TNF-alpha), protein kinase C (PKC) activators (TPA), and db-cyclic adenosine monophosphate (cAMP) on IL-8 and GRO-alpha production by immortalized GC1a and granulosa-lutein cells. METHOD OF STUDY: Confluent granulosa-lutein cells were placed in serum-free medium before incubated for 8 hr with the above-mentioned test agents. Finally, we measured IL-8 and GRO-alpha levels in the culture media using an enzyme-linked immunosorbent assay (ELISA). RESULTS: Treatment of granulosa-lutein cells with of IL-1alpha (1 nM), TNF-alpha (1 nM), TPA (1 nM) and db-cAMP (100 microM) produced higher levels of IL-8 than untreated cells by 8 hr (2274.7 +/- 146.3, 1489.8 +/- 190.1, 1452.9 +/- 152.7, 1313.6 +/- 48.4 pg/mL, respectively; control = 457.7 +/- 38.2 pg/mL; P < 0.001). Treatment of granulosa-lutein cells with 1 nM of IL-1alpha, TNFalpha, TPA, and db-cAMP (100 microM) resulted in higher levels of GRO-alpha than untreated cells by 8 hr (993.7 +/- 9.5, 171.4 +/- 6.5, 147.5 +/- 6.7, 472.4 +/- 16.2 pg/mL respectively; control = 73.8 +/- 8.2 pg/mL; P < 0.001). CONCLUSIONS: Our data strongly suggests roles for IL-1alpha, TNFalpha, and PKC activators in the inflammation-like mechanism of human ovulation. Furthermore, our study suggests a positive, but still debatable, role for cAMP in the same mechanism. SN - 1046-7408 UR - https://www.unboundmedicine.com/medline/citation/14506934/Possible_modulators_of_IL_8_and_GRO_alpha_production_by_granulosa_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=1046-7408&amp;date=2003&amp;volume=50&amp;issue=1&amp;spage=98 DB - PRIME DP - Unbound Medicine ER -