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Influence of LDL receptor gene mutations and the R3500Q mutation of the apoB gene on lipoprotein phenotype of familial hypercholesterolemic patients from a South European population.
Eur J Hum Genet. 2003 Dec; 11(12):959-65.EJ

Abstract

Few data are available on genotype-phenotype interactions among familial hypercholesterolemia (FH) patients in South European populations and there are no data about the influence of R3500Q mutation on lipoprotein phenotype compared to low-density lipoprotein receptor (LDLR) mutations. The objective of the study is to analyze the influence of mutations in the LDLR and apolipoprotein B (apoB) genes on lipoprotein phenotype among subjects clinically diagnosed of FH living in East Spain. In all, 113 FH index patients and 100 affected relatives were studied. Genetic diagnosis was carried out following a protocol based on Southern blot and PCR-SSCP analysis. A total of 118 FH subjects could be classified into three groups according to the type of LDLR mutations (null mutations, missense mutations affecting the ligand binding 3-5 repeat, and missense mutations outside this domain). In addition, the lipoprotein phenotype of these FH groups was compared with 19 heterozygous subjects with familial ligand-defective apoB (FDB), due to R3500Q mutation. FH patients carrying missense mutations affecting the ligand binding repeat 3-5 showed total and LDL cholesterol levels significantly higher than FH patients with missense mutations in other LDLR domains or FDB patients. FH subjects carrying null mutations showed lower high-density lipoprotein cholesterol plasma values compared to FH carrying missense mutations. FDB subjects showed the lowest total and LDL cholesterol plasma values. In conclusion, the type of LDLR gene mutation and R3500Q mutation influences the lipoprotein phenotype of FH population from East Spain.

Authors+Show Affiliations

Service of Endocrinology and Nutrition, Department of Medicine, Hospital Clínico Universitario, University of Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14508510

Citation

Real, José T., et al. "Influence of LDL Receptor Gene Mutations and the R3500Q Mutation of the apoB Gene On Lipoprotein Phenotype of Familial Hypercholesterolemic Patients From a South European Population." European Journal of Human Genetics : EJHG, vol. 11, no. 12, 2003, pp. 959-65.
Real JT, Chaves FJ, Ejarque I, et al. Influence of LDL receptor gene mutations and the R3500Q mutation of the apoB gene on lipoprotein phenotype of familial hypercholesterolemic patients from a South European population. Eur J Hum Genet. 2003;11(12):959-65.
Real, J. T., Chaves, F. J., Ejarque, I., García-García, A. B., Valldecabres, C., Ascaso, J. F., Armengod, M. E., & Carmena, R. (2003). Influence of LDL receptor gene mutations and the R3500Q mutation of the apoB gene on lipoprotein phenotype of familial hypercholesterolemic patients from a South European population. European Journal of Human Genetics : EJHG, 11(12), 959-65.
Real JT, et al. Influence of LDL Receptor Gene Mutations and the R3500Q Mutation of the apoB Gene On Lipoprotein Phenotype of Familial Hypercholesterolemic Patients From a South European Population. Eur J Hum Genet. 2003;11(12):959-65. PubMed PMID: 14508510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of LDL receptor gene mutations and the R3500Q mutation of the apoB gene on lipoprotein phenotype of familial hypercholesterolemic patients from a South European population. AU - Real,José T, AU - Chaves,Felipe J, AU - Ejarque,Ismael, AU - García-García,Ana B, AU - Valldecabres,Carmen, AU - Ascaso,Juan F, AU - Armengod,María E, AU - Carmena,Rafael, PY - 2003/9/26/pubmed PY - 2004/8/20/medline PY - 2003/9/26/entrez SP - 959 EP - 65 JF - European journal of human genetics : EJHG JO - Eur J Hum Genet VL - 11 IS - 12 N2 - Few data are available on genotype-phenotype interactions among familial hypercholesterolemia (FH) patients in South European populations and there are no data about the influence of R3500Q mutation on lipoprotein phenotype compared to low-density lipoprotein receptor (LDLR) mutations. The objective of the study is to analyze the influence of mutations in the LDLR and apolipoprotein B (apoB) genes on lipoprotein phenotype among subjects clinically diagnosed of FH living in East Spain. In all, 113 FH index patients and 100 affected relatives were studied. Genetic diagnosis was carried out following a protocol based on Southern blot and PCR-SSCP analysis. A total of 118 FH subjects could be classified into three groups according to the type of LDLR mutations (null mutations, missense mutations affecting the ligand binding 3-5 repeat, and missense mutations outside this domain). In addition, the lipoprotein phenotype of these FH groups was compared with 19 heterozygous subjects with familial ligand-defective apoB (FDB), due to R3500Q mutation. FH patients carrying missense mutations affecting the ligand binding repeat 3-5 showed total and LDL cholesterol levels significantly higher than FH patients with missense mutations in other LDLR domains or FDB patients. FH subjects carrying null mutations showed lower high-density lipoprotein cholesterol plasma values compared to FH carrying missense mutations. FDB subjects showed the lowest total and LDL cholesterol plasma values. In conclusion, the type of LDLR gene mutation and R3500Q mutation influences the lipoprotein phenotype of FH population from East Spain. SN - 1018-4813 UR - https://www.unboundmedicine.com/medline/citation/14508510/Influence_of_LDL_receptor_gene_mutations_and_the_R3500Q_mutation_of_the_apoB_gene_on_lipoprotein_phenotype_of_familial_hypercholesterolemic_patients_from_a_South_European_population_ L2 - https://medlineplus.gov/cholesterollevelswhatyouneedtoknow.html DB - PRIME DP - Unbound Medicine ER -