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Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene.
Expert Rev Mol Diagn. 2003 Sep; 3(5):669-75.ER

Abstract

A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

Authors+Show Affiliations

Advanced Biomedical Science and Technology Group, Oak Ridge National Laboratory, TN 37831-6101, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

14510186

Citation

Culha, Mustafa, et al. "Surface-enhanced Raman Scattering for Cancer Diagnostics: Detection of the BCL2 Gene." Expert Review of Molecular Diagnostics, vol. 3, no. 5, 2003, pp. 669-75.
Culha M, Stokes D, Vo-Dinh T. Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene. Expert Rev Mol Diagn. 2003;3(5):669-75.
Culha, M., Stokes, D., & Vo-Dinh, T. (2003). Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene. Expert Review of Molecular Diagnostics, 3(5), 669-75.
Culha M, Stokes D, Vo-Dinh T. Surface-enhanced Raman Scattering for Cancer Diagnostics: Detection of the BCL2 Gene. Expert Rev Mol Diagn. 2003;3(5):669-75. PubMed PMID: 14510186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene. AU - Culha,Mustafa, AU - Stokes,David, AU - Vo-Dinh,Tuan, PY - 2003/9/27/pubmed PY - 2004/2/27/medline PY - 2003/9/27/entrez SP - 669 EP - 75 JF - Expert review of molecular diagnostics JO - Expert Rev Mol Diagn VL - 3 IS - 5 N2 - A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated. SN - 1473-7159 UR - https://www.unboundmedicine.com/medline/citation/14510186/Surface_enhanced_Raman_scattering_for_cancer_diagnostics:_detection_of_the_BCL2_gene_ L2 - https://www.tandfonline.com/doi/full/10.1586/14737159.3.5.669 DB - PRIME DP - Unbound Medicine ER -