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Molecular cloning and characterization of a cDNA encoding cytochrome c oxidase subunit Va from the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae).
Arch Insect Biochem Physiol. 2003 Oct; 54(2):47-54.AI

Abstract

A cDNA encoding subunit Va of cytochrome c oxidase (EC 1.9.3.1) was cloned and characterized from a lesser grain borer (Rhyzopertha dominica) cDNA library. The complete cDNA consists of 693-bp and contains an open reading frame of 450-bp that encodes 150 amino acid residues. The sequence includes a 28-bp putative N-terminal and a 122-bp putative mature protein. The estimated molecular weight and pI for the predicted mature protein are 13,962 and 4.60, respectively. The cDNA-deduced amino acid sequence of the mature protein shows 73% identity to that of a corresponding subunit of African malaria mosquito (Anopheles gambiae) and 59% identity to that of the fruit fly (Drosophila melanogaster). In addition, 31% of all amino acid residues are conserved among six different animal species. Evolutionary distance analysis suggests that cytochrome c oxidase subunit Va from R. dominica is most similar to the corresponding subunit from the malaria mosquito. Northern analysis revealed a single 4.9-kb transcript that is much larger than that found in mammalian species.

Authors+Show Affiliations

Department of Entomology, Kansas State University, Manhattan, Kansas 66506-4004, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

14518003

Citation

Ayala, Jorge, et al. "Molecular Cloning and Characterization of a cDNA Encoding Cytochrome C Oxidase Subunit Va From the Lesser Grain Borer, Rhyzopertha Dominica (F.) (Coleoptera: Bostrichidae)." Archives of Insect Biochemistry and Physiology, vol. 54, no. 2, 2003, pp. 47-54.
Ayala J, Dowdy AK, Beeman RW, et al. Molecular cloning and characterization of a cDNA encoding cytochrome c oxidase subunit Va from the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). Arch Insect Biochem Physiol. 2003;54(2):47-54.
Ayala, J., Dowdy, A. K., Beeman, R. W., & Zhu, K. Y. (2003). Molecular cloning and characterization of a cDNA encoding cytochrome c oxidase subunit Va from the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). Archives of Insect Biochemistry and Physiology, 54(2), 47-54.
Ayala J, et al. Molecular Cloning and Characterization of a cDNA Encoding Cytochrome C Oxidase Subunit Va From the Lesser Grain Borer, Rhyzopertha Dominica (F.) (Coleoptera: Bostrichidae). Arch Insect Biochem Physiol. 2003;54(2):47-54. PubMed PMID: 14518003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and characterization of a cDNA encoding cytochrome c oxidase subunit Va from the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). AU - Ayala,Jorge, AU - Dowdy,Alan K, AU - Beeman,Richard W, AU - Zhu,Kun Yan, PY - 2003/10/1/pubmed PY - 2003/10/31/medline PY - 2003/10/1/entrez SP - 47 EP - 54 JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 54 IS - 2 N2 - A cDNA encoding subunit Va of cytochrome c oxidase (EC 1.9.3.1) was cloned and characterized from a lesser grain borer (Rhyzopertha dominica) cDNA library. The complete cDNA consists of 693-bp and contains an open reading frame of 450-bp that encodes 150 amino acid residues. The sequence includes a 28-bp putative N-terminal and a 122-bp putative mature protein. The estimated molecular weight and pI for the predicted mature protein are 13,962 and 4.60, respectively. The cDNA-deduced amino acid sequence of the mature protein shows 73% identity to that of a corresponding subunit of African malaria mosquito (Anopheles gambiae) and 59% identity to that of the fruit fly (Drosophila melanogaster). In addition, 31% of all amino acid residues are conserved among six different animal species. Evolutionary distance analysis suggests that cytochrome c oxidase subunit Va from R. dominica is most similar to the corresponding subunit from the malaria mosquito. Northern analysis revealed a single 4.9-kb transcript that is much larger than that found in mammalian species. SN - 0739-4462 UR - https://www.unboundmedicine.com/medline/citation/14518003/Molecular_cloning_and_characterization_of_a_cDNA_encoding_cytochrome_c_oxidase_subunit_Va_from_the_lesser_grain_borer_Rhyzopertha_dominica__F____Coleoptera:_Bostrichidae__ L2 - https://doi.org/10.1002/arch.10101 DB - PRIME DP - Unbound Medicine ER -