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Intradermal delivery of antisense oligonucleotides by the pulse depolarization iontophoretic system.
Biol Pharm Bull. 2003 Oct; 26(10):1461-6.BP

Abstract

The intradermal delivery of an antisense oligonucleotide was examined by iontophoresis. In this experiment, the antisense sequence of [(32)P]-labeled phosphodiester oligonucleotide ([(32)P]D-oligo, 18-mer) hybridizing to mouse interleukin 10 (IL-10) mRNA was used as a model D-oligo. In in vitro iontophoretic experiments, isolated hairless mouse skin was used with a horizontal diffusion cell. The enhancing effect of pulse depolarization (PDP) iontophoresis on the [(32)P]D-oligo permeation through the skin was better, and the skin irritation was less, than those of constant direct current (CDC) iontophoresis. The apparent fluxes of [(32)P]D-oligo were enhanced with the increasing current densities and [(32)P]D-oligo concentrations in the donor solution, whereas the enhanced flux decreased with the increasing NaCl concentrations in the donor solution. An optimum electric current was observed for the intradermal delivery of [(32)P]D-oligo, and intact [(32)P]D-oligo was detected within the skin after iontophoresis for 6 h. These results suggest that PDP iontophoresis may be useful for the intradermal delivery of antisense oligonucleotides.

Authors+Show Affiliations

School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14519955

Citation

Aramaki, Yukihiko, et al. "Intradermal Delivery of Antisense Oligonucleotides By the Pulse Depolarization Iontophoretic System." Biological & Pharmaceutical Bulletin, vol. 26, no. 10, 2003, pp. 1461-6.
Aramaki Y, Arima H, Takahashi M, et al. Intradermal delivery of antisense oligonucleotides by the pulse depolarization iontophoretic system. Biol Pharm Bull. 2003;26(10):1461-6.
Aramaki, Y., Arima, H., Takahashi, M., Miyazaki, E., Sakamoto, T., & Tsuchiya, S. (2003). Intradermal delivery of antisense oligonucleotides by the pulse depolarization iontophoretic system. Biological & Pharmaceutical Bulletin, 26(10), 1461-6.
Aramaki Y, et al. Intradermal Delivery of Antisense Oligonucleotides By the Pulse Depolarization Iontophoretic System. Biol Pharm Bull. 2003;26(10):1461-6. PubMed PMID: 14519955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intradermal delivery of antisense oligonucleotides by the pulse depolarization iontophoretic system. AU - Aramaki,Yukihiko, AU - Arima,Hidetoshi, AU - Takahashi,Mamiko, AU - Miyazaki,Eriko, AU - Sakamoto,Takatoshi, AU - Tsuchiya,Seishi, PY - 2003/10/2/pubmed PY - 2004/2/18/medline PY - 2003/10/2/entrez SP - 1461 EP - 6 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 26 IS - 10 N2 - The intradermal delivery of an antisense oligonucleotide was examined by iontophoresis. In this experiment, the antisense sequence of [(32)P]-labeled phosphodiester oligonucleotide ([(32)P]D-oligo, 18-mer) hybridizing to mouse interleukin 10 (IL-10) mRNA was used as a model D-oligo. In in vitro iontophoretic experiments, isolated hairless mouse skin was used with a horizontal diffusion cell. The enhancing effect of pulse depolarization (PDP) iontophoresis on the [(32)P]D-oligo permeation through the skin was better, and the skin irritation was less, than those of constant direct current (CDC) iontophoresis. The apparent fluxes of [(32)P]D-oligo were enhanced with the increasing current densities and [(32)P]D-oligo concentrations in the donor solution, whereas the enhanced flux decreased with the increasing NaCl concentrations in the donor solution. An optimum electric current was observed for the intradermal delivery of [(32)P]D-oligo, and intact [(32)P]D-oligo was detected within the skin after iontophoresis for 6 h. These results suggest that PDP iontophoresis may be useful for the intradermal delivery of antisense oligonucleotides. SN - 0918-6158 UR - https://www.unboundmedicine.com/medline/citation/14519955/Intradermal_delivery_of_antisense_oligonucleotides_by_the_pulse_depolarization_iontophoretic_system_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/bpb/26.1461?from=PubMed DB - PRIME DP - Unbound Medicine ER -