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Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis thaliana.
Planta. 2003 Jul; 217(3):507-16.P

Abstract

Expression of a cDNA encoding the castor bean (Ricinus communis L.) oleate Delta12-hydroxylase in the developing seeds of Arabidopsis thaliana (L.) Heynh. results in the synthesis of four novel hydroxy fatty acids. These have been previously identified as ricinoleic acid (12-hydroxy-octadec- cis-9-enoic acid: 18:1-OH), densipolic acid (12-hydroxy-octadec- cis-9,15-enoic acid: 18:2-OH), lesquerolic acid (14-hydroxy-eicos- cis-11-enoic acid: 20:1-OH) and auricolic acid (14-hydroxy-eicos- cis-11,17-enoic acid: 20:2-OH). Using mutant lines of Arabidopsis that lack the activity of the FAE1 condensing enzyme or FAD3 ER Delta-15-desaturase, we have shown that these enzymes are required for the synthesis of C20 hydroxy fatty acids and polyunsaturated hydroxy fatty acids, respectively. Analysis of the seed fatty acid composition of transformed plants demonstrated a dramatic increase in oleic acid (18:1) levels and a decrease in linoleic acid (18:2) content correlating to the levels of hydroxy fatty acid present in the seed. Plants in which FAD2 (ER Delta12-desaturase) activity was absent showed a decrease in 18:1 content and a slight increase in 18:2 levels corresponding to hydroxy fatty acid content. Expression of the castor hydroxylase protein in yeast indicates that this enzyme has a low level of fatty acid Delta12-desaturase activity. Lipase catalysed 1,3-specific lipolysis of triacylglycerol from transformed plants demonstrated that ricinoleic acid is not excluded from the sn-2 position of triacylglycerol, but is the only hydroxy fatty acid present at this position.

Authors+Show Affiliations

Department of Botany, University of British Columbia, Vancouver, Canada. msmith@mail.botany.ubc.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14520576

Citation

Smith, Mark A., et al. "Heterologous Expression of a Fatty Acid Hydroxylase Gene in Developing Seeds of Arabidopsis Thaliana." Planta, vol. 217, no. 3, 2003, pp. 507-16.
Smith MA, Moon H, Chowrira G, et al. Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis thaliana. Planta. 2003;217(3):507-16.
Smith, M. A., Moon, H., Chowrira, G., & Kunst, L. (2003). Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis thaliana. Planta, 217(3), 507-16.
Smith MA, et al. Heterologous Expression of a Fatty Acid Hydroxylase Gene in Developing Seeds of Arabidopsis Thaliana. Planta. 2003;217(3):507-16. PubMed PMID: 14520576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis thaliana. AU - Smith,Mark A, AU - Moon,Hangsik, AU - Chowrira,Gangamma, AU - Kunst,Ljerka, Y1 - 2003/03/18/ PY - 2002/11/19/received PY - 2003/02/12/accepted PY - 2003/10/2/pubmed PY - 2003/12/24/medline PY - 2003/10/2/entrez SP - 507 EP - 16 JF - Planta JO - Planta VL - 217 IS - 3 N2 - Expression of a cDNA encoding the castor bean (Ricinus communis L.) oleate Delta12-hydroxylase in the developing seeds of Arabidopsis thaliana (L.) Heynh. results in the synthesis of four novel hydroxy fatty acids. These have been previously identified as ricinoleic acid (12-hydroxy-octadec- cis-9-enoic acid: 18:1-OH), densipolic acid (12-hydroxy-octadec- cis-9,15-enoic acid: 18:2-OH), lesquerolic acid (14-hydroxy-eicos- cis-11-enoic acid: 20:1-OH) and auricolic acid (14-hydroxy-eicos- cis-11,17-enoic acid: 20:2-OH). Using mutant lines of Arabidopsis that lack the activity of the FAE1 condensing enzyme or FAD3 ER Delta-15-desaturase, we have shown that these enzymes are required for the synthesis of C20 hydroxy fatty acids and polyunsaturated hydroxy fatty acids, respectively. Analysis of the seed fatty acid composition of transformed plants demonstrated a dramatic increase in oleic acid (18:1) levels and a decrease in linoleic acid (18:2) content correlating to the levels of hydroxy fatty acid present in the seed. Plants in which FAD2 (ER Delta12-desaturase) activity was absent showed a decrease in 18:1 content and a slight increase in 18:2 levels corresponding to hydroxy fatty acid content. Expression of the castor hydroxylase protein in yeast indicates that this enzyme has a low level of fatty acid Delta12-desaturase activity. Lipase catalysed 1,3-specific lipolysis of triacylglycerol from transformed plants demonstrated that ricinoleic acid is not excluded from the sn-2 position of triacylglycerol, but is the only hydroxy fatty acid present at this position. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/14520576/Heterologous_expression_of_a_fatty_acid_hydroxylase_gene_in_developing_seeds_of_Arabidopsis_thaliana_ L2 - https://dx.doi.org/10.1007/s00425-003-1015-6 DB - PRIME DP - Unbound Medicine ER -