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N1-acetyl-N2-formyl-5-methoxykynuramine is a product of melatonin oxidation in rats.
J Pineal Res. 2003 Nov; 35(4):245-50.JP

Abstract

The product of melatonin oxidation, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), was synthesized and a method for its determination in biological samples was developed. High performance liquid chromatography (HPLC) with fluorescence detection provided good sensitivity and selectivity. Wavelengths of 350 and 480 nm were used for excitation and emission, respectively. Serum and retinal homogenates were extracted with chloroform prior to analysis by HPLC. Endogenous AFMK was detected in the retina of rats but the serum concentration of this melatonin metabolite was below the detection limit of the method for measurement. Retinal AFMK concentration was higher during the dark phase of the light/dark cycle, when the retinal melatonin content is maximal. Intraperitoneal administration of melatonin significantly increased serum and retinal AFMK levels. Formation of AFMK from melatonin was also confirmed by in vivo microdialysis with the probe implanted into the brain lateral ventricle. The study shows that AFMK is indeed a product of melatonin oxidation in vivo. The possible physiological significance of melatonin oxidation metabolic pathway is discussed.

Authors+Show Affiliations

Rozov SV
Sechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Saint-Petersburg, Russia.
Filatova EV
No affiliation info available
Orlov AA
No affiliation info available
Volkova AV
No affiliation info available
Zhloba AR
No affiliation info available
Blashko EL
No affiliation info available
Pozdeyev NV
No affiliation info available

MeSH

AnimalsKynuramineMelatoninOxidation-ReductionRatsRetina

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14521629

Citation

Rozov, Stanislav V., et al. "N1-acetyl-N2-formyl-5-methoxykynuramine Is a Product of Melatonin Oxidation in Rats." Journal of Pineal Research, vol. 35, no. 4, 2003, pp. 245-50.
Rozov SV, Filatova EV, Orlov AA, et al. N1-acetyl-N2-formyl-5-methoxykynuramine is a product of melatonin oxidation in rats. J Pineal Res. 2003;35(4):245-50.
Rozov, S. V., Filatova, E. V., Orlov, A. A., Volkova, A. V., Zhloba, A. R., Blashko, E. L., & Pozdeyev, N. V. (2003). N1-acetyl-N2-formyl-5-methoxykynuramine is a product of melatonin oxidation in rats. Journal of Pineal Research, 35(4), 245-50.
Rozov SV, et al. N1-acetyl-N2-formyl-5-methoxykynuramine Is a Product of Melatonin Oxidation in Rats. J Pineal Res. 2003;35(4):245-50. PubMed PMID: 14521629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N1-acetyl-N2-formyl-5-methoxykynuramine is a product of melatonin oxidation in rats. AU - Rozov,Stanislav V, AU - Filatova,Elena V, AU - Orlov,Alexandr A, AU - Volkova,Anvara V, AU - Zhloba,Alexand R A, AU - Blashko,Eduard L, AU - Pozdeyev,Nikita V, PY - 2003/10/3/pubmed PY - 2004/7/3/medline PY - 2003/10/3/entrez SP - 245 EP - 50 JF - Journal of pineal research JO - J Pineal Res VL - 35 IS - 4 N2 - The product of melatonin oxidation, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), was synthesized and a method for its determination in biological samples was developed. High performance liquid chromatography (HPLC) with fluorescence detection provided good sensitivity and selectivity. Wavelengths of 350 and 480 nm were used for excitation and emission, respectively. Serum and retinal homogenates were extracted with chloroform prior to analysis by HPLC. Endogenous AFMK was detected in the retina of rats but the serum concentration of this melatonin metabolite was below the detection limit of the method for measurement. Retinal AFMK concentration was higher during the dark phase of the light/dark cycle, when the retinal melatonin content is maximal. Intraperitoneal administration of melatonin significantly increased serum and retinal AFMK levels. Formation of AFMK from melatonin was also confirmed by in vivo microdialysis with the probe implanted into the brain lateral ventricle. The study shows that AFMK is indeed a product of melatonin oxidation in vivo. The possible physiological significance of melatonin oxidation metabolic pathway is discussed. SN - 0742-3098 UR - https://www.unboundmedicine.com/medline/citation/14521629/N1_acetyl_N2_formyl_5_methoxykynuramine_is_a_product_of_melatonin_oxidation_in_rats_ DB - PRIME DP - Unbound Medicine ER -