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Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends.
J Biol Chem. 2003 Dec 05; 278(49):48957-64.JB

Abstract

Saccharomyces cerevisiae Rad50, Mre11, and Xrs2 proteins are involved in homologous recombination, non-homologous end-joining, DNA damage checkpoint signaling, and telomere maintenance. These proteins form a stable complex that has nuclease, DNA binding, and DNA end recognition activities. Of the components of the Rad50.Mre11.Xrs2 complex, Xrs2 is the least characterized. The available evidence is consistent with the idea that Xrs2 recruits other protein factors in reactions that pertain to the biological functions of the Rad50.Mre11.Xrs2 complex. Here we present biochemical evidence that Xrs2 has an associated DNA-binding activity that is specific for DNA structures. We also define the contributions of Xrs2 to the activities of the Rad50.Mre11.Xrs2 complex. Importantly, we demonstrate that Xrs2 is critical for targeting of Rad50 and Mre11 to DNA ends. Thus, Xrs2 likely plays a direct role in the engagement of DNA substrates by the Rad50. Mre11.Xrs2 complex in various biological processes.

Authors+Show Affiliations

University of Texas Health Science Center, Department of Molecular Medicine, Institute of Biotechnology, San Antonio, Texas 78245, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14522986

Citation

Trujillo, Kelly M., et al. "Yeast Xrs2 Binds DNA and Helps Target Rad50 and Mre11 to DNA Ends." The Journal of Biological Chemistry, vol. 278, no. 49, 2003, pp. 48957-64.
Trujillo KM, Roh DH, Chen L, et al. Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends. J Biol Chem. 2003;278(49):48957-64.
Trujillo, K. M., Roh, D. H., Chen, L., Van Komen, S., Tomkinson, A., & Sung, P. (2003). Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends. The Journal of Biological Chemistry, 278(49), 48957-64.
Trujillo KM, et al. Yeast Xrs2 Binds DNA and Helps Target Rad50 and Mre11 to DNA Ends. J Biol Chem. 2003 Dec 5;278(49):48957-64. PubMed PMID: 14522986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends. AU - Trujillo,Kelly M, AU - Roh,Dong Hyun, AU - Chen,Ling, AU - Van Komen,Stephen, AU - Tomkinson,Alan, AU - Sung,Patrick, Y1 - 2003/09/30/ PY - 2003/10/3/pubmed PY - 2004/3/11/medline PY - 2003/10/3/entrez SP - 48957 EP - 64 JF - The Journal of biological chemistry JO - J Biol Chem VL - 278 IS - 49 N2 - Saccharomyces cerevisiae Rad50, Mre11, and Xrs2 proteins are involved in homologous recombination, non-homologous end-joining, DNA damage checkpoint signaling, and telomere maintenance. These proteins form a stable complex that has nuclease, DNA binding, and DNA end recognition activities. Of the components of the Rad50.Mre11.Xrs2 complex, Xrs2 is the least characterized. The available evidence is consistent with the idea that Xrs2 recruits other protein factors in reactions that pertain to the biological functions of the Rad50.Mre11.Xrs2 complex. Here we present biochemical evidence that Xrs2 has an associated DNA-binding activity that is specific for DNA structures. We also define the contributions of Xrs2 to the activities of the Rad50.Mre11.Xrs2 complex. Importantly, we demonstrate that Xrs2 is critical for targeting of Rad50 and Mre11 to DNA ends. Thus, Xrs2 likely plays a direct role in the engagement of DNA substrates by the Rad50. Mre11.Xrs2 complex in various biological processes. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/14522986/Yeast_xrs2_binds_DNA_and_helps_target_rad50_and_mre11_to_DNA_ends_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)75665-6 DB - PRIME DP - Unbound Medicine ER -