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Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter.
J Bacteriol. 2003 Oct; 185(20):5976-83.JB

Abstract

Pseudomonas aeruginosa contains two transcription regulators (LasR and RhlR) that, when complexed with their specific autoinducers (3-oxo-dodecanoyl-homoserine lactone and butanoyl-homoserine lactone, respectively) activate transcription of different virulence-associated traits. We studied the RhlR-dependent transcriptional regulation of the rhlAB operon encoding rhamnosyltransferase 1, an enzyme involved in the synthesis of the surfactant monorhamnolipid, and showed that RhlR binds to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer. Our data suggest that in the former case it activates transcription, whereas in the latter it acts as a transcriptional repressor of this promoter. RhlR seems to repress the transcription of other quorum-sensing-regulated genes; thus, RhlR repressor activity might be of importance in the finely regulated expression of P. aeruginosa virulence-associated traits.

Authors+Show Affiliations

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14526008

Citation

Medina, Gerardo, et al. "Mechanism of Pseudomonas Aeruginosa RhlR Transcriptional Regulation of the rhlAB Promoter." Journal of Bacteriology, vol. 185, no. 20, 2003, pp. 5976-83.
Medina G, Juárez K, Valderrama B, et al. Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J Bacteriol. 2003;185(20):5976-83.
Medina, G., Juárez, K., Valderrama, B., & Soberón-Chávez, G. (2003). Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. Journal of Bacteriology, 185(20), 5976-83.
Medina G, et al. Mechanism of Pseudomonas Aeruginosa RhlR Transcriptional Regulation of the rhlAB Promoter. J Bacteriol. 2003;185(20):5976-83. PubMed PMID: 14526008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. AU - Medina,Gerardo, AU - Juárez,Katy, AU - Valderrama,Brenda, AU - Soberón-Chávez,Gloria, PY - 2003/10/4/pubmed PY - 2003/10/31/medline PY - 2003/10/4/entrez SP - 5976 EP - 83 JF - Journal of bacteriology JO - J. Bacteriol. VL - 185 IS - 20 N2 - Pseudomonas aeruginosa contains two transcription regulators (LasR and RhlR) that, when complexed with their specific autoinducers (3-oxo-dodecanoyl-homoserine lactone and butanoyl-homoserine lactone, respectively) activate transcription of different virulence-associated traits. We studied the RhlR-dependent transcriptional regulation of the rhlAB operon encoding rhamnosyltransferase 1, an enzyme involved in the synthesis of the surfactant monorhamnolipid, and showed that RhlR binds to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer. Our data suggest that in the former case it activates transcription, whereas in the latter it acts as a transcriptional repressor of this promoter. RhlR seems to repress the transcription of other quorum-sensing-regulated genes; thus, RhlR repressor activity might be of importance in the finely regulated expression of P. aeruginosa virulence-associated traits. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/14526008/Mechanism_of_Pseudomonas_aeruginosa_RhlR_transcriptional_regulation_of_the_rhlAB_promoter_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=14526008 DB - PRIME DP - Unbound Medicine ER -