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Induction of increased intracellular calcium in astrocytes by glutamate through activating NMDA and AMPA receptors.

Abstract

To study the effect of glutamate on the intracellular calcium signal of pure cultured rat astrocytes and the role of NMDA and AMPA receptors in the procedure, the change of calcium signal was investigated by monitoring the fluctuation of intracellular Ca2+ concentration ([Ca2+]i) on the basis of Fura-2 single cell fluorescent ratio (F345/F380). The changes in the effect of glutamate on the intracellular calcium signal were observed after blockage of NMDA and (or) AMPA receptors. It was found that L-glutamate could induce an increased [Ca2+]i in most of the cells in concentration- and time-dependent manner. D-(-)-2-amino-5-phosphonopentanoic acid (D-AP-5, a selective antagonist of the NMDA receptor) and 6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX, a selective antagonist of the AMPA receptor) could abolish the effects of NMDA and AMPA respectively. The treatment of D-AP-5 and CNQX simultaneously or respectively could attenuate the effect of L-glutamate at varying degrees. All these indicated that glutamate could modulate intracellular Ca2+ of pure cultured rat astrocytes through different pathways. The activation of NMDA and AMPA receptors took part in the complex mechanisms.

Authors+Show Affiliations

Department of Pathophysiology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14526426

Citation

Zhang, Qi, et al. "Induction of Increased Intracellular Calcium in Astrocytes By Glutamate Through Activating NMDA and AMPA Receptors." Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, vol. 23, no. 3, 2003, pp. 254-7.
Zhang Q, Hu B, Sun S, et al. Induction of increased intracellular calcium in astrocytes by glutamate through activating NMDA and AMPA receptors. J Huazhong Univ Sci Technolog Med Sci. 2003;23(3):254-7.
Zhang, Q., Hu, B., Sun, S., & Tong, E. (2003). Induction of increased intracellular calcium in astrocytes by glutamate through activating NMDA and AMPA receptors. Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, 23(3), 254-7.
Zhang Q, et al. Induction of Increased Intracellular Calcium in Astrocytes By Glutamate Through Activating NMDA and AMPA Receptors. J Huazhong Univ Sci Technolog Med Sci. 2003;23(3):254-7. PubMed PMID: 14526426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of increased intracellular calcium in astrocytes by glutamate through activating NMDA and AMPA receptors. AU - Zhang,Qi, AU - Hu,Bo, AU - Sun,Shenggang, AU - Tong,Etang, PY - 2003/10/7/pubmed PY - 2003/12/3/medline PY - 2003/10/7/entrez SP - 254 EP - 7 JF - Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban JO - J Huazhong Univ Sci Technolog Med Sci VL - 23 IS - 3 N2 - To study the effect of glutamate on the intracellular calcium signal of pure cultured rat astrocytes and the role of NMDA and AMPA receptors in the procedure, the change of calcium signal was investigated by monitoring the fluctuation of intracellular Ca2+ concentration ([Ca2+]i) on the basis of Fura-2 single cell fluorescent ratio (F345/F380). The changes in the effect of glutamate on the intracellular calcium signal were observed after blockage of NMDA and (or) AMPA receptors. It was found that L-glutamate could induce an increased [Ca2+]i in most of the cells in concentration- and time-dependent manner. D-(-)-2-amino-5-phosphonopentanoic acid (D-AP-5, a selective antagonist of the NMDA receptor) and 6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX, a selective antagonist of the AMPA receptor) could abolish the effects of NMDA and AMPA respectively. The treatment of D-AP-5 and CNQX simultaneously or respectively could attenuate the effect of L-glutamate at varying degrees. All these indicated that glutamate could modulate intracellular Ca2+ of pure cultured rat astrocytes through different pathways. The activation of NMDA and AMPA receptors took part in the complex mechanisms. SN - 1672-0733 UR - https://www.unboundmedicine.com/medline/citation/14526426/Induction_of_increased_intracellular_calcium_in_astrocytes_by_glutamate_through_activating_NMDA_and_AMPA_receptors_ DB - PRIME DP - Unbound Medicine ER -