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Growth phase and SpvR regulation of transcription of Salmonella typhimurium spvABC virulence genes.
Microb Pathog. 1992 Aug; 13(2):133-43.MP

Abstract

The 90 kb virulence plasmid of Salmonella typhimurium is required for bacterial growth beyond the small intestine to deeper tissues such as the spleen and liver of orally inoculated mice. We constructed transcriptional lacZ fusions within the cloned plasmid-borne virulence genes spvA, spvB and spvC of S. typhimurium to demonstrate that spvR encodes a trans-acting positive regulator for the transcription of spvA, spvB and spvC. Data suggesting that the activation of spvABC transcription is dependent on the growth phase of both S. typhimurium and Escherichia coli grown in Luria Broth (LB) are also presented. Complementation experiments for virulence in mice confirmed that at least spvR and spvC are virulence genes and further suggested that the spvRABC gene cluster consists of at least three transcriptional units containing spvR, spvC and spvABC, respectively. Reinitiation of transcription at spvC was confirmed in vitro, using a lacZ fusion, and was shown to be independent of SpvR-mediated control in LB.

Authors+Show Affiliations

Institut Pasteur, Unité INSERM 199, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1453926

Citation

Coynault, C, et al. "Growth Phase and SpvR Regulation of Transcription of Salmonella Typhimurium spvABC Virulence Genes." Microbial Pathogenesis, vol. 13, no. 2, 1992, pp. 133-43.
Coynault C, Robbe-Saule V, Popoff MY, et al. Growth phase and SpvR regulation of transcription of Salmonella typhimurium spvABC virulence genes. Microb Pathog. 1992;13(2):133-43.
Coynault, C., Robbe-Saule, V., Popoff, M. Y., & Norel, F. (1992). Growth phase and SpvR regulation of transcription of Salmonella typhimurium spvABC virulence genes. Microbial Pathogenesis, 13(2), 133-43.
Coynault C, et al. Growth Phase and SpvR Regulation of Transcription of Salmonella Typhimurium spvABC Virulence Genes. Microb Pathog. 1992;13(2):133-43. PubMed PMID: 1453926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth phase and SpvR regulation of transcription of Salmonella typhimurium spvABC virulence genes. AU - Coynault,C, AU - Robbe-Saule,V, AU - Popoff,M Y, AU - Norel,F, PY - 1992/8/1/pubmed PY - 1992/8/1/medline PY - 1992/8/1/entrez SP - 133 EP - 43 JF - Microbial pathogenesis JO - Microb Pathog VL - 13 IS - 2 N2 - The 90 kb virulence plasmid of Salmonella typhimurium is required for bacterial growth beyond the small intestine to deeper tissues such as the spleen and liver of orally inoculated mice. We constructed transcriptional lacZ fusions within the cloned plasmid-borne virulence genes spvA, spvB and spvC of S. typhimurium to demonstrate that spvR encodes a trans-acting positive regulator for the transcription of spvA, spvB and spvC. Data suggesting that the activation of spvABC transcription is dependent on the growth phase of both S. typhimurium and Escherichia coli grown in Luria Broth (LB) are also presented. Complementation experiments for virulence in mice confirmed that at least spvR and spvC are virulence genes and further suggested that the spvRABC gene cluster consists of at least three transcriptional units containing spvR, spvC and spvABC, respectively. Reinitiation of transcription at spvC was confirmed in vitro, using a lacZ fusion, and was shown to be independent of SpvR-mediated control in LB. SN - 0882-4010 UR - https://www.unboundmedicine.com/medline/citation/1453926/Growth_phase_and_SpvR_regulation_of_transcription_of_Salmonella_typhimurium_spvABC_virulence_genes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0882-4010(92)90073-W DB - PRIME DP - Unbound Medicine ER -