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Transcriptional analysis of the rpoE gene encoding extracytoplasmic stress response sigma factor sigmaE in Salmonella enterica serovar Typhimurium.
FEMS Microbiol Lett. 2003 Sep 26; 226(2):307-14.FM

Abstract

The rpoE gene of Salmonella enterica serovar Typhimurium (S. Typhimurium), which encodes the extracytoplasmic stress response sigma factor sigmaE, is critically important for the virulence of S. Typhimurium. We analysed expression of rpoE by wild-type and mutant bacteria grown in different conditions by S1-nuclease mapping using RNA, and using in vivo reporter gene fusions. Three promoters, rpoEp1, rpoEp2 and rpoEp3, were located upstream of the S. Typhimurium rpoE gene. The promoters were differentially expressed during growth and under several stress conditions including cold shock. Expression from the rpoEp3 promoter was absent in an S. Typhimurium rpoE mutant, demonstrating its dependence upon sigmaE. The level of mRNA corresponding to rpoEp3 was also higher in a cpxR mutant, indicating a negative regulation of the promoter by the Cpx system. Using this rpoE-dependent promoter, we optimised a two-plasmid system for identification of promoters recognised by S. Typhimurium sigmaE. The rpoEp3 promoter was active in the Escherichia coli two-plasmid system and has an identical transcription start point as in S. Typhimurium but only after induction of S. Typhimurium rpoE expression.

Authors+Show Affiliations

Institute of Molecular Biology, Slovak Academy of Science, Dubravska cesta 21, 845 51 Bratislava, Slovak Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14553927

Citation

Miticka, Henrieta, et al. "Transcriptional Analysis of the rpoE Gene Encoding Extracytoplasmic Stress Response Sigma Factor sigmaE in Salmonella Enterica Serovar Typhimurium." FEMS Microbiology Letters, vol. 226, no. 2, 2003, pp. 307-14.
Miticka H, Rowley G, Rezuchova B, et al. Transcriptional analysis of the rpoE gene encoding extracytoplasmic stress response sigma factor sigmaE in Salmonella enterica serovar Typhimurium. FEMS Microbiol Lett. 2003;226(2):307-14.
Miticka, H., Rowley, G., Rezuchova, B., Homerova, D., Humphreys, S., Farn, J., Roberts, M., & Kormanec, J. (2003). Transcriptional analysis of the rpoE gene encoding extracytoplasmic stress response sigma factor sigmaE in Salmonella enterica serovar Typhimurium. FEMS Microbiology Letters, 226(2), 307-14.
Miticka H, et al. Transcriptional Analysis of the rpoE Gene Encoding Extracytoplasmic Stress Response Sigma Factor sigmaE in Salmonella Enterica Serovar Typhimurium. FEMS Microbiol Lett. 2003 Sep 26;226(2):307-14. PubMed PMID: 14553927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional analysis of the rpoE gene encoding extracytoplasmic stress response sigma factor sigmaE in Salmonella enterica serovar Typhimurium. AU - Miticka,Henrieta, AU - Rowley,Gary, AU - Rezuchova,Bronislava, AU - Homerova,Dagmar, AU - Humphreys,Sue, AU - Farn,Jaci, AU - Roberts,Mark, AU - Kormanec,Jan, PY - 2003/10/14/pubmed PY - 2003/12/18/medline PY - 2003/10/14/entrez SP - 307 EP - 14 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 226 IS - 2 N2 - The rpoE gene of Salmonella enterica serovar Typhimurium (S. Typhimurium), which encodes the extracytoplasmic stress response sigma factor sigmaE, is critically important for the virulence of S. Typhimurium. We analysed expression of rpoE by wild-type and mutant bacteria grown in different conditions by S1-nuclease mapping using RNA, and using in vivo reporter gene fusions. Three promoters, rpoEp1, rpoEp2 and rpoEp3, were located upstream of the S. Typhimurium rpoE gene. The promoters were differentially expressed during growth and under several stress conditions including cold shock. Expression from the rpoEp3 promoter was absent in an S. Typhimurium rpoE mutant, demonstrating its dependence upon sigmaE. The level of mRNA corresponding to rpoEp3 was also higher in a cpxR mutant, indicating a negative regulation of the promoter by the Cpx system. Using this rpoE-dependent promoter, we optimised a two-plasmid system for identification of promoters recognised by S. Typhimurium sigmaE. The rpoEp3 promoter was active in the Escherichia coli two-plasmid system and has an identical transcription start point as in S. Typhimurium but only after induction of S. Typhimurium rpoE expression. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/14553927/Transcriptional_analysis_of_the_rpoE_gene_encoding_extracytoplasmic_stress_response_sigma_factor_sigmaE_in_Salmonella_enterica_serovar_Typhimurium_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/S0378-1097(03)00600-1 DB - PRIME DP - Unbound Medicine ER -