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HBV DNA integration and HBV-transcript expression in non-B, non-C hepatocellular carcinoma in Japan.
J Med Virol 2003; 71(4):492-8JM

Abstract

Few studies have examined the etiology of hepatocellular carcinoma (HCC) in patients without hepatitis virus infection. We evaluated the role of occult hepatitis B virus (HBV) infection in the development of HCC in Japanese patients without hepatitis B surface antigen (HBsAg) and antibodies to hepatitis C antigen (anti-HCV). Twenty-one HBsAg negative and anti-HCV negative (non-B, non-C) patients with HCC were studied. HBV DNA in serum and HBV transcripts in liver were examined by polymerase chain reaction (PCR) or reverse transcription and PCR. HBV DNA integration was examined by Southern blot analysis or cassette-ligation-mediated PCR as described previously. p53 mutations were examined by direct sequencing. HBV DNA was not detected in serum from any patients. HBV-related transcripts were detected in 5 of 7 HCCs from patients with antibodies to hepatitis core antigen (anti-HBc) and in 3 of 14 HCCs from patients without anti-HBc (P = 0.0261). HBV DNA was integrated into human genome in two non-B, non-C HCCs. Of the 14 patients without anti-HBc, 5 had a history of excessive alcohol intake. In exons 5 through 8 of the p53 gene, mutations were detected in 2 of 8 HCCs with HBV-transcripts and in 5 of 13 HCCs without such transcripts. p53 mutation at codon 159 was found in 2 of 6 patients with excessive alcohol intake without HBV-transcripts. These results suggested that occult HBV infection might play an important role in hepatocarcinogenesis in non-B, non-C patients with anti-HBc and that excessive alcohol intake might be related to HCC in non-B, non-C patients in Japan.

Authors+Show Affiliations

Department of Hepatology, Osaka City University Graduate School of Medicine, Osaka, Japan. atamori@med.osaka-cu.ac.jp

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14556260

Citation

Tamori, Akihiro, et al. "HBV DNA Integration and HBV-transcript Expression in non-B, non-C Hepatocellular Carcinoma in Japan." Journal of Medical Virology, vol. 71, no. 4, 2003, pp. 492-8.
Tamori A, Nishiguchi S, Kubo S, et al. HBV DNA integration and HBV-transcript expression in non-B, non-C hepatocellular carcinoma in Japan. J Med Virol. 2003;71(4):492-8.
Tamori, A., Nishiguchi, S., Kubo, S., Narimatsu, T., Habu, D., Takeda, T., ... Shiomi, S. (2003). HBV DNA integration and HBV-transcript expression in non-B, non-C hepatocellular carcinoma in Japan. Journal of Medical Virology, 71(4), pp. 492-8.
Tamori A, et al. HBV DNA Integration and HBV-transcript Expression in non-B, non-C Hepatocellular Carcinoma in Japan. J Med Virol. 2003;71(4):492-8. PubMed PMID: 14556260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HBV DNA integration and HBV-transcript expression in non-B, non-C hepatocellular carcinoma in Japan. AU - Tamori,Akihiro, AU - Nishiguchi,Shuhei, AU - Kubo,Shoji, AU - Narimatsu,Takashi, AU - Habu,Daiki, AU - Takeda,Tadashi, AU - Hirohashi,Kazuhiro, AU - Shiomi,Susumu, PY - 2003/10/14/pubmed PY - 2004/1/21/medline PY - 2003/10/14/entrez SP - 492 EP - 8 JF - Journal of medical virology JO - J. Med. Virol. VL - 71 IS - 4 N2 - Few studies have examined the etiology of hepatocellular carcinoma (HCC) in patients without hepatitis virus infection. We evaluated the role of occult hepatitis B virus (HBV) infection in the development of HCC in Japanese patients without hepatitis B surface antigen (HBsAg) and antibodies to hepatitis C antigen (anti-HCV). Twenty-one HBsAg negative and anti-HCV negative (non-B, non-C) patients with HCC were studied. HBV DNA in serum and HBV transcripts in liver were examined by polymerase chain reaction (PCR) or reverse transcription and PCR. HBV DNA integration was examined by Southern blot analysis or cassette-ligation-mediated PCR as described previously. p53 mutations were examined by direct sequencing. HBV DNA was not detected in serum from any patients. HBV-related transcripts were detected in 5 of 7 HCCs from patients with antibodies to hepatitis core antigen (anti-HBc) and in 3 of 14 HCCs from patients without anti-HBc (P = 0.0261). HBV DNA was integrated into human genome in two non-B, non-C HCCs. Of the 14 patients without anti-HBc, 5 had a history of excessive alcohol intake. In exons 5 through 8 of the p53 gene, mutations were detected in 2 of 8 HCCs with HBV-transcripts and in 5 of 13 HCCs without such transcripts. p53 mutation at codon 159 was found in 2 of 6 patients with excessive alcohol intake without HBV-transcripts. These results suggested that occult HBV infection might play an important role in hepatocarcinogenesis in non-B, non-C patients with anti-HBc and that excessive alcohol intake might be related to HCC in non-B, non-C patients in Japan. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/14556260/HBV_DNA_integration_and_HBV_transcript_expression_in_non_B_non_C_hepatocellular_carcinoma_in_Japan_ L2 - https://doi.org/10.1002/jmv.10514 DB - PRIME DP - Unbound Medicine ER -