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Discrimination between arabica and robusta coffee species on the basis of their amino acid enantiomers.
J Agric Food Chem. 2003 Oct 22; 51(22):6495-501.JA

Abstract

This work reports the results for the composition of robusta and arabica coffee species in terms of their amino acid enantiomers in the green and roasted states. The analyses were conducted for the free amino acids, as well as for the amino acids obtained after acid hydrolysis. The amino acids were extracted/hydrolyzed and isolated by SPE on strong cation exchange columns, derivatized to their N-ethoxycarbonylheptafluorobutyl esters, and analyzed by gas chromatography/FID on a Chirasil l-Val column. Multivariate analyses applied to the results showed that the free amino acids can be used as a tool for discrimination between coffee species, with a special reference to l-glutamic acid, l-tryptophan, and pipecolic acid. There is also some evidence that these compounds can be used for discrimination between green coffees subjected to different postharvest processes. It is also shown that the amino acid levels observed after acid hydrolysis can be used for the same purposes, although displaying less discriminatory power.

Authors+Show Affiliations

REQUIMTE, Serviço de Bromatologia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, Portugal. sucasal@ff.up.ptNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14558768

Citation

Casal, Susana, et al. "Discrimination Between Arabica and Robusta Coffee Species On the Basis of Their Amino Acid Enantiomers." Journal of Agricultural and Food Chemistry, vol. 51, no. 22, 2003, pp. 6495-501.
Casal S, Alves MR, Mendes E, et al. Discrimination between arabica and robusta coffee species on the basis of their amino acid enantiomers. J Agric Food Chem. 2003;51(22):6495-501.
Casal, S., Alves, M. R., Mendes, E., Oliveira, M. B., & Ferreira, M. A. (2003). Discrimination between arabica and robusta coffee species on the basis of their amino acid enantiomers. Journal of Agricultural and Food Chemistry, 51(22), 6495-501.
Casal S, et al. Discrimination Between Arabica and Robusta Coffee Species On the Basis of Their Amino Acid Enantiomers. J Agric Food Chem. 2003 Oct 22;51(22):6495-501. PubMed PMID: 14558768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discrimination between arabica and robusta coffee species on the basis of their amino acid enantiomers. AU - Casal,Susana, AU - Alves,M Rui, AU - Mendes,Eulália, AU - Oliveira,M Beatriz P P, AU - Ferreira,Margarida A, PY - 2003/10/16/pubmed PY - 2003/12/12/medline PY - 2003/10/16/entrez SP - 6495 EP - 501 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 51 IS - 22 N2 - This work reports the results for the composition of robusta and arabica coffee species in terms of their amino acid enantiomers in the green and roasted states. The analyses were conducted for the free amino acids, as well as for the amino acids obtained after acid hydrolysis. The amino acids were extracted/hydrolyzed and isolated by SPE on strong cation exchange columns, derivatized to their N-ethoxycarbonylheptafluorobutyl esters, and analyzed by gas chromatography/FID on a Chirasil l-Val column. Multivariate analyses applied to the results showed that the free amino acids can be used as a tool for discrimination between coffee species, with a special reference to l-glutamic acid, l-tryptophan, and pipecolic acid. There is also some evidence that these compounds can be used for discrimination between green coffees subjected to different postharvest processes. It is also shown that the amino acid levels observed after acid hydrolysis can be used for the same purposes, although displaying less discriminatory power. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/14558768/Discrimination_between_arabica_and_robusta_coffee_species_on_the_basis_of_their_amino_acid_enantiomers_ L2 - https://dx.doi.org/10.1021/jf034354w DB - PRIME DP - Unbound Medicine ER -