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Cloning and characterization of a basic phospholipase A2 homologue from Micrurus corallinus (coral snake) venom gland.
Toxicon. 2003 Sep; 42(3):249-55.T

Abstract

During the cloning of abundant cDNAs expressed in the Micrurus corallinus coral snake venom gland, several putative toxins, including a phospholipase A2 homologue cDNA (clone V2), were identified. The V2 cDNA clone codes for a potential coral snake toxin with a signal peptide of 27 amino acid residues plus a predicted mature protein with 119 amino acid residues. The deduced protein is highly similar to known phospholipases A2, with seven deduced S-S bridges at the same conserved positions. This protein was expressed in Escherichia coli as a His-tagged protein that allowed the rapid purification of the recombinant protein. This protein was used to generate antibodies, which recognized the recombinant protein in Western blot. This antiserum was used to screen a large number of venoms, showing a ubiquitous distribution of immunorelated proteins in all elapidic venoms but not in the viperidic Bothrops jararaca venom. This is the first description of a complete primary structure of a phospholipase A2 homologue deduced by cDNA cloning from a coral snake.

Authors+Show Affiliations

Centro de Biotecnologia, Instituto Butantan, Av. Vital Brazil, 1500, CEP:05503-900, São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14559075

Citation

de Oliveira, Ursula Castro, et al. "Cloning and Characterization of a Basic Phospholipase A2 Homologue From Micrurus Corallinus (coral Snake) Venom Gland." Toxicon : Official Journal of the International Society On Toxinology, vol. 42, no. 3, 2003, pp. 249-55.
de Oliveira UC, Assui A, da Silva AR, et al. Cloning and characterization of a basic phospholipase A2 homologue from Micrurus corallinus (coral snake) venom gland. Toxicon. 2003;42(3):249-55.
de Oliveira, U. C., Assui, A., da Silva, A. R., de Oliveira, J. S., & Ho, P. L. (2003). Cloning and characterization of a basic phospholipase A2 homologue from Micrurus corallinus (coral snake) venom gland. Toxicon : Official Journal of the International Society On Toxinology, 42(3), 249-55.
de Oliveira UC, et al. Cloning and Characterization of a Basic Phospholipase A2 Homologue From Micrurus Corallinus (coral Snake) Venom Gland. Toxicon. 2003;42(3):249-55. PubMed PMID: 14559075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of a basic phospholipase A2 homologue from Micrurus corallinus (coral snake) venom gland. AU - de Oliveira,Ursula Castro, AU - Assui,Alessandra, AU - da Silva,Alvaro Rossan de Brandão Prieto, AU - de Oliveira,Jane Silveira, AU - Ho,Paulo Lee, PY - 2003/10/16/pubmed PY - 2003/12/16/medline PY - 2003/10/16/entrez SP - 249 EP - 55 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 42 IS - 3 N2 - During the cloning of abundant cDNAs expressed in the Micrurus corallinus coral snake venom gland, several putative toxins, including a phospholipase A2 homologue cDNA (clone V2), were identified. The V2 cDNA clone codes for a potential coral snake toxin with a signal peptide of 27 amino acid residues plus a predicted mature protein with 119 amino acid residues. The deduced protein is highly similar to known phospholipases A2, with seven deduced S-S bridges at the same conserved positions. This protein was expressed in Escherichia coli as a His-tagged protein that allowed the rapid purification of the recombinant protein. This protein was used to generate antibodies, which recognized the recombinant protein in Western blot. This antiserum was used to screen a large number of venoms, showing a ubiquitous distribution of immunorelated proteins in all elapidic venoms but not in the viperidic Bothrops jararaca venom. This is the first description of a complete primary structure of a phospholipase A2 homologue deduced by cDNA cloning from a coral snake. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/14559075/Cloning_and_characterization_of_a_basic_phospholipase_A2_homologue_from_Micrurus_corallinus__coral_snake__venom_gland_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041010103001399 DB - PRIME DP - Unbound Medicine ER -