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[Effects of Huobahuagen tablet on the expression of interleukin-3 receptor mRNA in asthmatic guinea pig bronchoalveolar lavage fluid and eosinophil infiltration].
Di Yi Jun Yi Da Xue Xue Bao. 2003 Oct; 23(10):1069-73.DY

Abstract

OBJECTIVE

To investigate the effects of Huobahuagen tablet on the expression of interleukin-3 (IL-3) receptor mRNA in bronchoalveolar lavage fluid (BALF) and infiltration of eosinophil (Eos) in the airway of asthmatic guinea pigs.

METHODS

Thirty-two healthy guinea pigs were randomized into 4 equal groups, the control group, asthmatic group, dexamethasone therapy group and Huobahuagen tablet therapy group. Asthma was induced in the latter 3 groups which were challenged with the asthma-inducing agents and at the same time received treatments as indicated. BALF were collected subsequently from the guinea pigs for examining the total cell number and cell classification, and histopathologic examination of the lung tissue was performed. Semi-quantitative analysis with reverse transcriptional-polymerase chain reaction (RT-PCR) of IL-3 receptor mRNA in the BALF was performed.

RESULTS

Compared with the control and the asthmatic group, the number of Eos in the BALF of Huobahuagen tablet therapy group was significantly lower (P<0.05 and P<0.01, respectively). In spite of the presence of hyperemia and edema in the lung tissues of the Huobahuagen tablet therapy group, Eos infiltration was less severe than that in the asthmatic group. As found by RT-PCR, the quantity of IL-3 receptor mRNA in the BALF of the Huobahuagen tablet therapy group did not significantly differ from that in the dexamethasone therapy group, but was significantly higher than that in both the control and asthmatic group (P<0.01).

CONCLUSION

Huobahuagen tablet can significantly lower the number of Eos in the airway of asthmatic guinea pigs, a finding that may potentially serve as the elementary theoretical basis for clinical therapy of asthma.

Authors+Show Affiliations

Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. liangluo93@hotmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

14559697

Citation

Luo, Liang, et al. "[Effects of Huobahuagen Tablet On the Expression of Interleukin-3 Receptor mRNA in Asthmatic Guinea Pig Bronchoalveolar Lavage Fluid and Eosinophil Infiltration]." Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, vol. 23, no. 10, 2003, pp. 1069-73.
Luo L, Luo YL, Lai WY. [Effects of Huobahuagen tablet on the expression of interleukin-3 receptor mRNA in asthmatic guinea pig bronchoalveolar lavage fluid and eosinophil infiltration]. Di Yi Jun Yi Da Xue Xue Bao. 2003;23(10):1069-73.
Luo, L., Luo, Y. L., & Lai, W. Y. (2003). [Effects of Huobahuagen tablet on the expression of interleukin-3 receptor mRNA in asthmatic guinea pig bronchoalveolar lavage fluid and eosinophil infiltration]. Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, 23(10), 1069-73.
Luo L, Luo YL, Lai WY. [Effects of Huobahuagen Tablet On the Expression of Interleukin-3 Receptor mRNA in Asthmatic Guinea Pig Bronchoalveolar Lavage Fluid and Eosinophil Infiltration]. Di Yi Jun Yi Da Xue Xue Bao. 2003;23(10):1069-73. PubMed PMID: 14559697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Huobahuagen tablet on the expression of interleukin-3 receptor mRNA in asthmatic guinea pig bronchoalveolar lavage fluid and eosinophil infiltration]. AU - Luo,Liang, AU - Luo,Ya-ling, AU - Lai,Wen-yan, PY - 2003/10/16/pubmed PY - 2004/2/18/medline PY - 2003/10/16/entrez SP - 1069 EP - 73 JF - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA JO - Di Yi Jun Yi Da Xue Xue Bao VL - 23 IS - 10 N2 - OBJECTIVE: To investigate the effects of Huobahuagen tablet on the expression of interleukin-3 (IL-3) receptor mRNA in bronchoalveolar lavage fluid (BALF) and infiltration of eosinophil (Eos) in the airway of asthmatic guinea pigs. METHODS: Thirty-two healthy guinea pigs were randomized into 4 equal groups, the control group, asthmatic group, dexamethasone therapy group and Huobahuagen tablet therapy group. Asthma was induced in the latter 3 groups which were challenged with the asthma-inducing agents and at the same time received treatments as indicated. BALF were collected subsequently from the guinea pigs for examining the total cell number and cell classification, and histopathologic examination of the lung tissue was performed. Semi-quantitative analysis with reverse transcriptional-polymerase chain reaction (RT-PCR) of IL-3 receptor mRNA in the BALF was performed. RESULTS: Compared with the control and the asthmatic group, the number of Eos in the BALF of Huobahuagen tablet therapy group was significantly lower (P<0.05 and P<0.01, respectively). In spite of the presence of hyperemia and edema in the lung tissues of the Huobahuagen tablet therapy group, Eos infiltration was less severe than that in the asthmatic group. As found by RT-PCR, the quantity of IL-3 receptor mRNA in the BALF of the Huobahuagen tablet therapy group did not significantly differ from that in the dexamethasone therapy group, but was significantly higher than that in both the control and asthmatic group (P<0.01). CONCLUSION: Huobahuagen tablet can significantly lower the number of Eos in the airway of asthmatic guinea pigs, a finding that may potentially serve as the elementary theoretical basis for clinical therapy of asthma. SN - 1000-2588 UR - https://www.unboundmedicine.com/medline/citation/14559697/[Effects_of_Huobahuagen_tablet_on_the_expression_of_interleukin_3_receptor_mRNA_in_asthmatic_guinea_pig_bronchoalveolar_lavage_fluid_and_eosinophil_infiltration]_ DB - PRIME DP - Unbound Medicine ER -