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Localisation of P2Y1 and P2Y4 receptors in dorsal root, nodose and trigeminal ganglia of the rat.
Histochem Cell Biol 2003; 120(5):415-26HC

Abstract

The presence and distribution of P2Y (nucleotide) receptor subtypes in rat sensory neurons has been investigated. RT-PCR showed that P2Y(1), P2Y(2), P2Y(4) and P2Y(6) receptor mRNA is expressed in sensory ganglia [dorsal root ganglion (DRG), nodose ganglion (NG) and trigeminal ganglion (TG)]. The regional and cellular distribution of P2Y(1) and P2Y(4) receptor proteins in these ganglia was investigated using immunohistochemistry. P2Y(1) polyclonal antibodies stained over 80% of the sensory neurons, particularly the small-diameter (neurofilament-negative) neurons. The P2Y(4) receptor antibody stained more medium- and large- (neurofilament-positive) diameter neurons than small-diameter neurons. P2Y(1) and P2Y(4) receptor immunoreactivity (P2Y(1)-IR and P2Y(4)-IR) was often coexpressed with P2X(3) receptor immunoreactivity (P2X(3)-IR) in subpopulations of neurons. Double immunohistochemistry showed that 73-84% of P2X(3) receptor-positive neurons also stained for the P2Y(1) receptor in DRG, TG and NG while only 25-35% also stained for the P2Y(4) receptor. Subpopulations of P2Y(1)-IR neurons were coexpressed with NF200, CGRP and IB(4); most P2Y(4)-IR neurons were coexpressed with NF200, while only a few neurons were coexpressed with CGRP (10-20%) or with IB(4) (1-2%). The results suggest that P2Y as well as P2X receptor subtypes contribute to purinergic signalling in sensory ganglia.

Authors+Show Affiliations

Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, NW3 2PF, London, UK.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14564529

Citation

Ruan, Huai Zhen, and Geoffrey Burnstock. "Localisation of P2Y1 and P2Y4 Receptors in Dorsal Root, Nodose and Trigeminal Ganglia of the Rat." Histochemistry and Cell Biology, vol. 120, no. 5, 2003, pp. 415-26.
Ruan HZ, Burnstock G. Localisation of P2Y1 and P2Y4 receptors in dorsal root, nodose and trigeminal ganglia of the rat. Histochem Cell Biol. 2003;120(5):415-26.
Ruan, H. Z., & Burnstock, G. (2003). Localisation of P2Y1 and P2Y4 receptors in dorsal root, nodose and trigeminal ganglia of the rat. Histochemistry and Cell Biology, 120(5), pp. 415-26.
Ruan HZ, Burnstock G. Localisation of P2Y1 and P2Y4 Receptors in Dorsal Root, Nodose and Trigeminal Ganglia of the Rat. Histochem Cell Biol. 2003;120(5):415-26. PubMed PMID: 14564529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localisation of P2Y1 and P2Y4 receptors in dorsal root, nodose and trigeminal ganglia of the rat. AU - Ruan,Huai Zhen, AU - Burnstock,Geoffrey, Y1 - 2003/10/15/ PY - 2003/09/16/accepted PY - 2003/10/18/pubmed PY - 2004/4/7/medline PY - 2003/10/18/entrez SP - 415 EP - 26 JF - Histochemistry and cell biology JO - Histochem. Cell Biol. VL - 120 IS - 5 N2 - The presence and distribution of P2Y (nucleotide) receptor subtypes in rat sensory neurons has been investigated. RT-PCR showed that P2Y(1), P2Y(2), P2Y(4) and P2Y(6) receptor mRNA is expressed in sensory ganglia [dorsal root ganglion (DRG), nodose ganglion (NG) and trigeminal ganglion (TG)]. The regional and cellular distribution of P2Y(1) and P2Y(4) receptor proteins in these ganglia was investigated using immunohistochemistry. P2Y(1) polyclonal antibodies stained over 80% of the sensory neurons, particularly the small-diameter (neurofilament-negative) neurons. The P2Y(4) receptor antibody stained more medium- and large- (neurofilament-positive) diameter neurons than small-diameter neurons. P2Y(1) and P2Y(4) receptor immunoreactivity (P2Y(1)-IR and P2Y(4)-IR) was often coexpressed with P2X(3) receptor immunoreactivity (P2X(3)-IR) in subpopulations of neurons. Double immunohistochemistry showed that 73-84% of P2X(3) receptor-positive neurons also stained for the P2Y(1) receptor in DRG, TG and NG while only 25-35% also stained for the P2Y(4) receptor. Subpopulations of P2Y(1)-IR neurons were coexpressed with NF200, CGRP and IB(4); most P2Y(4)-IR neurons were coexpressed with NF200, while only a few neurons were coexpressed with CGRP (10-20%) or with IB(4) (1-2%). The results suggest that P2Y as well as P2X receptor subtypes contribute to purinergic signalling in sensory ganglia. SN - 0948-6143 UR - https://www.unboundmedicine.com/medline/citation/14564529/Localisation_of_P2Y1_and_P2Y4_receptors_in_dorsal_root_nodose_and_trigeminal_ganglia_of_the_rat_ L2 - https://dx.doi.org/10.1007/s00418-003-0579-3 DB - PRIME DP - Unbound Medicine ER -