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Glial cell line-derived neurotrophic factor normalizes neurochemical changes in injured dorsal root ganglion neurons and prevents the expression of experimental neuropathic pain.
Neuroscience. 2003; 121(3):815-24.N

Abstract

Glial cell line-derived neurotrophic factor (GDNF) is necessary for the development of sensory neurons, and appears to be critical for the survival of dorsal root ganglion (DRG) cells that bind the lectin IB4. Intrathecal infusion of GDNF has been shown to prevent and reverse the behavioral expression of experimental neuropathic pain arising from injury to spinal nerves. This effect of GDNF has been attributed to a blockade of the expression of the voltage gated, tetrodotoxin-sensitive sodium channel subtype, Na(V)1.3, in the injured DRG. Here we report that GDNF given intrathecally via osmotic-pump to nerve-injured rats (L5/L6 spinal nerve ligation) prevented the changes in a variety of neurochemical markers in the DRG upon injury. They include a loss of binding of IB4, downregulation of the purinergic receptor P2X(3), upregulation of galanin and neuropeptide Y immunoreactivity in large diameter DRG cells, and expression of the transcription factor ATF3. GDNF infusion concomitantly prevented the development of spinal nerve ligation-induced tactile hypersensitivity and thermal hyperalgesia. These observations suggest that high dose, exogenous GDNF has a broad neuroprotective role in injured primary afferent. The receptor(s) that mediates these effects of GDNF is not known. GDNF's ability to block neuropathic pain states is not likely to be specific to Na(V)1.3 expression.

Authors+Show Affiliations

Department of Pharmacology, University of Arizona, Tucson 85724, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14568039

Citation

Wang, R, et al. "Glial Cell Line-derived Neurotrophic Factor Normalizes Neurochemical Changes in Injured Dorsal Root Ganglion Neurons and Prevents the Expression of Experimental Neuropathic Pain." Neuroscience, vol. 121, no. 3, 2003, pp. 815-24.
Wang R, Guo W, Ossipov MH, et al. Glial cell line-derived neurotrophic factor normalizes neurochemical changes in injured dorsal root ganglion neurons and prevents the expression of experimental neuropathic pain. Neuroscience. 2003;121(3):815-24.
Wang, R., Guo, W., Ossipov, M. H., Vanderah, T. W., Porreca, F., & Lai, J. (2003). Glial cell line-derived neurotrophic factor normalizes neurochemical changes in injured dorsal root ganglion neurons and prevents the expression of experimental neuropathic pain. Neuroscience, 121(3), 815-24.
Wang R, et al. Glial Cell Line-derived Neurotrophic Factor Normalizes Neurochemical Changes in Injured Dorsal Root Ganglion Neurons and Prevents the Expression of Experimental Neuropathic Pain. Neuroscience. 2003;121(3):815-24. PubMed PMID: 14568039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glial cell line-derived neurotrophic factor normalizes neurochemical changes in injured dorsal root ganglion neurons and prevents the expression of experimental neuropathic pain. AU - Wang,R, AU - Guo,W, AU - Ossipov,M H, AU - Vanderah,T W, AU - Porreca,F, AU - Lai,J, PY - 2003/10/22/pubmed PY - 2004/1/10/medline PY - 2003/10/22/entrez SP - 815 EP - 24 JF - Neuroscience JO - Neuroscience VL - 121 IS - 3 N2 - Glial cell line-derived neurotrophic factor (GDNF) is necessary for the development of sensory neurons, and appears to be critical for the survival of dorsal root ganglion (DRG) cells that bind the lectin IB4. Intrathecal infusion of GDNF has been shown to prevent and reverse the behavioral expression of experimental neuropathic pain arising from injury to spinal nerves. This effect of GDNF has been attributed to a blockade of the expression of the voltage gated, tetrodotoxin-sensitive sodium channel subtype, Na(V)1.3, in the injured DRG. Here we report that GDNF given intrathecally via osmotic-pump to nerve-injured rats (L5/L6 spinal nerve ligation) prevented the changes in a variety of neurochemical markers in the DRG upon injury. They include a loss of binding of IB4, downregulation of the purinergic receptor P2X(3), upregulation of galanin and neuropeptide Y immunoreactivity in large diameter DRG cells, and expression of the transcription factor ATF3. GDNF infusion concomitantly prevented the development of spinal nerve ligation-induced tactile hypersensitivity and thermal hyperalgesia. These observations suggest that high dose, exogenous GDNF has a broad neuroprotective role in injured primary afferent. The receptor(s) that mediates these effects of GDNF is not known. GDNF's ability to block neuropathic pain states is not likely to be specific to Na(V)1.3 expression. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/14568039/Glial_cell_line_derived_neurotrophic_factor_normalizes_neurochemical_changes_in_injured_dorsal_root_ganglion_neurons_and_prevents_the_expression_of_experimental_neuropathic_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306452203004913 DB - PRIME DP - Unbound Medicine ER -