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A single-fibre study of the relationship between MHC and TnC isoform composition in rat skeletal muscle.
Biochem J. 2004 Feb 15; 378(Pt 1):269-74.BJ

Abstract

In the present study, we investigated the possibility that MHC (myosin heavy chain) and TnC (troponin C) isoforms exist in specific combinations in rat-skeletal-muscle fibres. Single fibres (numbering 245) from soleus (predominantly slow-twitch) and sternomastoid (predominantly fast-twitch) muscles of adult rats were analysed for MHC and TnC isoform composition, using alanine-SDS/PAGE for separating MHC isoforms, and a novel method (based on the previously reported influence of Ca2+ on the mobility of Ca2+-binding proteins in SDS gels) for unequivocal identification of TnC isoforms in single-fibre segments. In this study, all fibres that contained only one MHC isoform (slow or fast) contained only the matching TnC isoform and all fibres that contained multiple fast MHC isoforms contained only the fast TnC isoform. Fibres expressing both slow and fast MHC isoforms displayed either both TnC isoforms or only one TnC isoform of a type depending on the relative proportion of fast/slow MHC present. Our results suggest a close relationship between MHC and TnC isoform composition in non-transforming skeletal muscles of adult rat.

Authors+Show Affiliations

Muscle Cell Biochemistry Laboratory, School of Biomedical Sciences, Victoria University of Technology, P.O. Box 14428, MCMC, Melbourne, VIC 8001, Australia. brett.oconnell@research.vu.edu.auNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14572306

Citation

O'Connell, Brett, et al. "A Single-fibre Study of the Relationship Between MHC and TnC Isoform Composition in Rat Skeletal Muscle." The Biochemical Journal, vol. 378, no. Pt 1, 2004, pp. 269-74.
O'Connell B, Nguyen LT, Stephenson GM. A single-fibre study of the relationship between MHC and TnC isoform composition in rat skeletal muscle. Biochem J. 2004;378(Pt 1):269-74.
O'Connell, B., Nguyen, L. T., & Stephenson, G. M. (2004). A single-fibre study of the relationship between MHC and TnC isoform composition in rat skeletal muscle. The Biochemical Journal, 378(Pt 1), 269-74.
O'Connell B, Nguyen LT, Stephenson GM. A Single-fibre Study of the Relationship Between MHC and TnC Isoform Composition in Rat Skeletal Muscle. Biochem J. 2004 Feb 15;378(Pt 1):269-74. PubMed PMID: 14572306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A single-fibre study of the relationship between MHC and TnC isoform composition in rat skeletal muscle. AU - O'Connell,Brett, AU - Nguyen,Long T, AU - Stephenson,Gabriela M M, PY - 2003/10/22/accepted PY - 2003/10/15/revised PY - 2003/08/01/received PY - 2003/10/24/pubmed PY - 2004/4/23/medline PY - 2003/10/24/entrez SP - 269 EP - 74 JF - The Biochemical journal JO - Biochem J VL - 378 IS - Pt 1 N2 - In the present study, we investigated the possibility that MHC (myosin heavy chain) and TnC (troponin C) isoforms exist in specific combinations in rat-skeletal-muscle fibres. Single fibres (numbering 245) from soleus (predominantly slow-twitch) and sternomastoid (predominantly fast-twitch) muscles of adult rats were analysed for MHC and TnC isoform composition, using alanine-SDS/PAGE for separating MHC isoforms, and a novel method (based on the previously reported influence of Ca2+ on the mobility of Ca2+-binding proteins in SDS gels) for unequivocal identification of TnC isoforms in single-fibre segments. In this study, all fibres that contained only one MHC isoform (slow or fast) contained only the matching TnC isoform and all fibres that contained multiple fast MHC isoforms contained only the fast TnC isoform. Fibres expressing both slow and fast MHC isoforms displayed either both TnC isoforms or only one TnC isoform of a type depending on the relative proportion of fast/slow MHC present. Our results suggest a close relationship between MHC and TnC isoform composition in non-transforming skeletal muscles of adult rat. SN - 1470-8728 UR - https://www.unboundmedicine.com/medline/citation/14572306/A_single_fibre_study_of_the_relationship_between_MHC_and_TnC_isoform_composition_in_rat_skeletal_muscle_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/BJ20031170 DB - PRIME DP - Unbound Medicine ER -