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[Effect of angiotensin II on tissue factor expression in human peripheral blood monocytes and its mechanisms].
Zhonghua Xue Ye Xue Za Zhi. 2003 Sep; 24(9):470-3.ZX

Abstract

OBJECTIVE

To elucidate the effect of angiotensin II (AngII) on the expression of tissue factor (TF) by monocytes and its mechanisms.

METHODS

Monocytes were isolated from healthy volunteers by Ficoll-Hypaque gradient and Percoll, and cultured in RPMI-1640. Procoagulant activity (PCA) was determined by one-stage clotting method, TF antigen by ELISA. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the TF gene mRNA. The levels of IkappaBalpha was detected by Western blot. Electrophoretic mobility shift assays (EMSA) were performed to evaluate the activity of NF-kappaB.

RESULTS

AngII (10(-9) - 10(-7) mol/L) significantly increased monocyte PCA, TF antigen and TF mRNA expression in a dose and time dependent manner. Losartan (10(-6) - 10(-5) mol/L) significantly inhibited the effects of AngII on TF activity, antigen and mRNA expression in a dose-dependent effects. Staurosporine (2.5 x 10(-7) mol/L) and genistein (4 x 10(-5) mol/L) lowered TF level of monocytes (P < 0.05). Western blot analysis revealed that after exposure to AngII (10(-7) mol/L), IkappaBalpha level decreased at 15 min, reached nadir at 60 min, and recovered at 180 min. EMSA showed NF-kappaB binding activity increased at 15 min, reached peak at 60 min, and recovered at 180 min. Pyrrolidine dithiocarbamate (PDTC, 10(-4) mol/L), an inhibitor of NF-kappaB, or AT1R antagonist losartan (10(-5)mol/L) inhibited AngII-induced NF-kappaB translocation.

CONCLUSIONS

AngII could induce the expression of TF in human monocytes, and this effect was mediated by AT1R. The PKC pathway played the most important role in AngII-induced TF expression. The activation of NF-kappaB was involved in TF expression in monocytes.

Authors+Show Affiliations

Department of Physiology, Xiangya Medical College, Central South University, Changsha 410078, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

14575590

Citation

He, Mei-xia, et al. "[Effect of Angiotensin II On Tissue Factor Expression in Human Peripheral Blood Monocytes and Its Mechanisms]." Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi, vol. 24, no. 9, 2003, pp. 470-3.
He MX, He XF, Xie QZ, et al. [Effect of angiotensin II on tissue factor expression in human peripheral blood monocytes and its mechanisms]. Zhonghua Xue Ye Xue Za Zhi. 2003;24(9):470-3.
He, M. X., He, X. F., Xie, Q. Z., Chen, F. P., & He, S. L. (2003). [Effect of angiotensin II on tissue factor expression in human peripheral blood monocytes and its mechanisms]. Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi, 24(9), 470-3.
He MX, et al. [Effect of Angiotensin II On Tissue Factor Expression in Human Peripheral Blood Monocytes and Its Mechanisms]. Zhonghua Xue Ye Xue Za Zhi. 2003;24(9):470-3. PubMed PMID: 14575590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of angiotensin II on tissue factor expression in human peripheral blood monocytes and its mechanisms]. AU - He,Mei-xia, AU - He,Xiao-fan, AU - Xie,Qin-zhi, AU - Chen,Fang-ping, AU - He,Shi-lin, PY - 2003/10/25/pubmed PY - 2004/2/19/medline PY - 2003/10/25/entrez SP - 470 EP - 3 JF - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi JO - Zhonghua Xue Ye Xue Za Zhi VL - 24 IS - 9 N2 - OBJECTIVE: To elucidate the effect of angiotensin II (AngII) on the expression of tissue factor (TF) by monocytes and its mechanisms. METHODS: Monocytes were isolated from healthy volunteers by Ficoll-Hypaque gradient and Percoll, and cultured in RPMI-1640. Procoagulant activity (PCA) was determined by one-stage clotting method, TF antigen by ELISA. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the TF gene mRNA. The levels of IkappaBalpha was detected by Western blot. Electrophoretic mobility shift assays (EMSA) were performed to evaluate the activity of NF-kappaB. RESULTS: AngII (10(-9) - 10(-7) mol/L) significantly increased monocyte PCA, TF antigen and TF mRNA expression in a dose and time dependent manner. Losartan (10(-6) - 10(-5) mol/L) significantly inhibited the effects of AngII on TF activity, antigen and mRNA expression in a dose-dependent effects. Staurosporine (2.5 x 10(-7) mol/L) and genistein (4 x 10(-5) mol/L) lowered TF level of monocytes (P < 0.05). Western blot analysis revealed that after exposure to AngII (10(-7) mol/L), IkappaBalpha level decreased at 15 min, reached nadir at 60 min, and recovered at 180 min. EMSA showed NF-kappaB binding activity increased at 15 min, reached peak at 60 min, and recovered at 180 min. Pyrrolidine dithiocarbamate (PDTC, 10(-4) mol/L), an inhibitor of NF-kappaB, or AT1R antagonist losartan (10(-5)mol/L) inhibited AngII-induced NF-kappaB translocation. CONCLUSIONS: AngII could induce the expression of TF in human monocytes, and this effect was mediated by AT1R. The PKC pathway played the most important role in AngII-induced TF expression. The activation of NF-kappaB was involved in TF expression in monocytes. SN - 0253-2727 UR - https://www.unboundmedicine.com/medline/citation/14575590/[Effect_of_angiotensin_II_on_tissue_factor_expression_in_human_peripheral_blood_monocytes_and_its_mechanisms]_ L2 - http://www.diseaseinfosearch.org/result/2708 DB - PRIME DP - Unbound Medicine ER -