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Influence of temperature and high dietary linoleic acid content on esterification, elongation, and desaturation of PUFA in Atlantic salmon hepatocytes.
Lipids 2003; 38(8):833-40L

Abstract

The esterification, desaturation, and elongation of [1-14C]18:3n-3, [1-14C]18:2n-6, and [1-14C]20:5n-3 at 5 and at 12 degrees C were studied using cultivated hepatocytes from Atlantic salmon. The salmon were fed diets, in which 0, 50, or 100% of the supplementary fish oil had been replaced by soybean oil, for 950 day-degrees at 5 and 12 degrees C. The endogenous percentage of 18:2n-6 in hepatocyte lipids was 2% in cells from fish fed a diet with 100% of the supplemental lipid from fish oil, and it was slightly less than 25% in cells from fish fed the diet with 100% of the supplemental lipid from soybean oil. Furthermore, the percentages of 20:3n-6 and 20:4n-6 were significantly higher in hepatocytes from fish fed on soybean oil than they were in those of fish fed on fish oil. The percentages of 20:5n-3 and 22:6n-3, on the other hand, were lower. The endogenous levels of n-6 FA were not significantly correlated with the total amounts of radiolabeled FA esterified in hepatocyte lipids. The main radiolabeled products formed from 18:2n-6 were 20:2n-6 and 20:3n-6. The level of the important eicosanoid precursor 20:4n-6 was twice as high in hepatocyte phospholipids from fish fed the 100% soybean oil diet as it was in hepatocytes from fish fed the diet with 100% of supplemental lipid from fish oil. The main products formed from 18:3n-3 were 20:4n-3, 20:5n-3, and 22:6n-3. High levels of dietary 18:2n-6 do allow, or even seem to increase, the production of 22:6n-3 from 18:3n-3 in hepatocytes. The main products formed from 20:5n-3 were 22:5n-3 and 22:6n-3. The production of 22:6n-3 from 20:5n-3 was higher at 5 degrees C than at 12 degrees C. The percentage of 24:5n-3 was higher at 5 degrees C than it was at 12 degrees C, as was the ratio of 24:5 to 22:5. These results suggest that the elongation rate of 22:5n-3 to 24:5n-3 is higher at the lower temperature.

Authors+Show Affiliations

AKVAFORSK (Institute of Aquaculture Research AS), NO-1432 As, Norway. bente.ruyter@akvaforsk.nlh.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14577662

Citation

Ruyter, B, et al. "Influence of Temperature and High Dietary Linoleic Acid Content On Esterification, Elongation, and Desaturation of PUFA in Atlantic Salmon Hepatocytes." Lipids, vol. 38, no. 8, 2003, pp. 833-40.
Ruyter B, Røsjø C, Grisdale-Helland B, et al. Influence of temperature and high dietary linoleic acid content on esterification, elongation, and desaturation of PUFA in Atlantic salmon hepatocytes. Lipids. 2003;38(8):833-40.
Ruyter, B., Røsjø, C., Grisdale-Helland, B., Rosenlund, G., Obach, A., & Thomassen, M. S. (2003). Influence of temperature and high dietary linoleic acid content on esterification, elongation, and desaturation of PUFA in Atlantic salmon hepatocytes. Lipids, 38(8), pp. 833-40.
Ruyter B, et al. Influence of Temperature and High Dietary Linoleic Acid Content On Esterification, Elongation, and Desaturation of PUFA in Atlantic Salmon Hepatocytes. Lipids. 2003;38(8):833-40. PubMed PMID: 14577662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of temperature and high dietary linoleic acid content on esterification, elongation, and desaturation of PUFA in Atlantic salmon hepatocytes. AU - Ruyter,B, AU - Røsjø,C, AU - Grisdale-Helland,B, AU - Rosenlund,G, AU - Obach,A, AU - Thomassen,M S, PY - 2003/10/28/pubmed PY - 2004/2/18/medline PY - 2003/10/28/entrez SP - 833 EP - 40 JF - Lipids JO - Lipids VL - 38 IS - 8 N2 - The esterification, desaturation, and elongation of [1-14C]18:3n-3, [1-14C]18:2n-6, and [1-14C]20:5n-3 at 5 and at 12 degrees C were studied using cultivated hepatocytes from Atlantic salmon. The salmon were fed diets, in which 0, 50, or 100% of the supplementary fish oil had been replaced by soybean oil, for 950 day-degrees at 5 and 12 degrees C. The endogenous percentage of 18:2n-6 in hepatocyte lipids was 2% in cells from fish fed a diet with 100% of the supplemental lipid from fish oil, and it was slightly less than 25% in cells from fish fed the diet with 100% of the supplemental lipid from soybean oil. Furthermore, the percentages of 20:3n-6 and 20:4n-6 were significantly higher in hepatocytes from fish fed on soybean oil than they were in those of fish fed on fish oil. The percentages of 20:5n-3 and 22:6n-3, on the other hand, were lower. The endogenous levels of n-6 FA were not significantly correlated with the total amounts of radiolabeled FA esterified in hepatocyte lipids. The main radiolabeled products formed from 18:2n-6 were 20:2n-6 and 20:3n-6. The level of the important eicosanoid precursor 20:4n-6 was twice as high in hepatocyte phospholipids from fish fed the 100% soybean oil diet as it was in hepatocytes from fish fed the diet with 100% of supplemental lipid from fish oil. The main products formed from 18:3n-3 were 20:4n-3, 20:5n-3, and 22:6n-3. High levels of dietary 18:2n-6 do allow, or even seem to increase, the production of 22:6n-3 from 18:3n-3 in hepatocytes. The main products formed from 20:5n-3 were 22:5n-3 and 22:6n-3. The production of 22:6n-3 from 20:5n-3 was higher at 5 degrees C than at 12 degrees C. The percentage of 24:5n-3 was higher at 5 degrees C than it was at 12 degrees C, as was the ratio of 24:5 to 22:5. These results suggest that the elongation rate of 22:5n-3 to 24:5n-3 is higher at the lower temperature. SN - 0024-4201 UR - https://www.unboundmedicine.com/medline/citation/14577662/Influence_of_temperature_and_high_dietary_linoleic_acid_content_on_esterification_elongation_and_desaturation_of_PUFA_in_Atlantic_salmon_hepatocytes_ L2 - https://link.springer.com/article/10.1007/s11745-003-1133-6 DB - PRIME DP - Unbound Medicine ER -