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[Efficient production of wheat alien translocation lines and characterization by molecular cytogenetics].
Yi Chuan Xue Bao. 2003 Jul; 30(7):619-24.YC

Abstract

The Triticum aestivum-Leymus mollis and T. aestivum-Thinopyrum intermedium translocation lines were induced by gametocidal chromosome 3C derived from Aegilops triuncialis and gamma-ray irradiated pollens of a T. aestivum-Th. intermedium addition line TAI-14 with a lower dosage (10Gy), respectively. By genomic in situ hybridization (GISH) analysis, three T. aestivum-L. mollis translocation lines (WM-10, WM-43 and WM-47) and three deletion lines (WM-18, WM-43 and WM-44) were selected from 59 F2 plants derived from a cross combination of T. aestivum-L. mollis substitution line M8724-8-13 x T. aestivum-Ae. triunicalis 3C chromosome addition line. The frequency of translocation lines produced and total frequency of chromosome structural variation occurred were 5.08% and 8.47%, respectively. Two of the three translocation lines, WM-10 and WM-43 all were heterozygous translocation lines carrying one T. aestivum-L. mollis Robertsonian translocation chromosome, but the translocation chromosome in the two lines were different in morphology. The other one, WM-47 was a double heterozygous translocation with two different translocation chromosomes. By the C-banding, one of the three translocation lines was identified, the translocation chromosome consisted of 7DL of wheat and a chromosome arm of L. mollis. In addition, wheat chromosome deletions were observed in some plants. In another cross combination involved in common wheat and T. aestivum-Th. intermedium addition line, two alien terminal non-Robertsonian translocation lines (WI-21 and WI-68) were identified from 69 F2 plants by C-banding and GISH, and the percentage of translocation line was 2.90%. By C-banding analysis, the translocation chromosomes involved in wheat chromosomes 3A and 4A in the two lines, respectively. These results indicate that inducing wheat alien translocation line by gametocidal chromosome and irradiated pollens all are efficient methods.

Authors+Show Affiliations

Wang XP
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. xpwang@genetics.ac.cn
Chu JH
No affiliation info available
Zhang XQ
No affiliation info available

MeSH

Chromosome BandingIn Situ HybridizationTranslocation, GeneticTriticum

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

14579529

Citation

Wang, Xian-Ping, et al. "[Efficient Production of Wheat Alien Translocation Lines and Characterization By Molecular Cytogenetics]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 30, no. 7, 2003, pp. 619-24.
Wang XP, Chu JH, Zhang XQ. [Efficient production of wheat alien translocation lines and characterization by molecular cytogenetics]. Yi Chuan Xue Bao. 2003;30(7):619-24.
Wang, X. P., Chu, J. H., & Zhang, X. Q. (2003). [Efficient production of wheat alien translocation lines and characterization by molecular cytogenetics]. Yi Chuan Xue Bao = Acta Genetica Sinica, 30(7), 619-24.
Wang XP, Chu JH, Zhang XQ. [Efficient Production of Wheat Alien Translocation Lines and Characterization By Molecular Cytogenetics]. Yi Chuan Xue Bao. 2003;30(7):619-24. PubMed PMID: 14579529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Efficient production of wheat alien translocation lines and characterization by molecular cytogenetics]. AU - Wang,Xian-Ping, AU - Chu,Jing-Hua, AU - Zhang,Xiang-Qi, PY - 2003/10/29/pubmed PY - 2003/12/12/medline PY - 2003/10/29/entrez SP - 619 EP - 24 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 30 IS - 7 N2 - The Triticum aestivum-Leymus mollis and T. aestivum-Thinopyrum intermedium translocation lines were induced by gametocidal chromosome 3C derived from Aegilops triuncialis and gamma-ray irradiated pollens of a T. aestivum-Th. intermedium addition line TAI-14 with a lower dosage (10Gy), respectively. By genomic in situ hybridization (GISH) analysis, three T. aestivum-L. mollis translocation lines (WM-10, WM-43 and WM-47) and three deletion lines (WM-18, WM-43 and WM-44) were selected from 59 F2 plants derived from a cross combination of T. aestivum-L. mollis substitution line M8724-8-13 x T. aestivum-Ae. triunicalis 3C chromosome addition line. The frequency of translocation lines produced and total frequency of chromosome structural variation occurred were 5.08% and 8.47%, respectively. Two of the three translocation lines, WM-10 and WM-43 all were heterozygous translocation lines carrying one T. aestivum-L. mollis Robertsonian translocation chromosome, but the translocation chromosome in the two lines were different in morphology. The other one, WM-47 was a double heterozygous translocation with two different translocation chromosomes. By the C-banding, one of the three translocation lines was identified, the translocation chromosome consisted of 7DL of wheat and a chromosome arm of L. mollis. In addition, wheat chromosome deletions were observed in some plants. In another cross combination involved in common wheat and T. aestivum-Th. intermedium addition line, two alien terminal non-Robertsonian translocation lines (WI-21 and WI-68) were identified from 69 F2 plants by C-banding and GISH, and the percentage of translocation line was 2.90%. By C-banding analysis, the translocation chromosomes involved in wheat chromosomes 3A and 4A in the two lines, respectively. These results indicate that inducing wheat alien translocation line by gametocidal chromosome and irradiated pollens all are efficient methods. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/14579529/[Efficient_production_of_wheat_alien_translocation_lines_and_characterization_by_molecular_cytogenetics]_ DB - PRIME DP - Unbound Medicine ER -