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Production of intracellular IL-2, TNF-alpha, and IFN-gamma by T cells in B-CLL.
Cytometry B Clin Cytom. 2003 Nov; 56(1):23-9.CB

Abstract

BACKGROUND

Recent evidence indicates that the slowly expanding population of CD5(+) B cells that characterizes B-cell chronic lymphocytic leukemia (B-CLL) could be related to defects in the response to cytokine produced by T cells that regulate apoptosis. We studied the intracellular expressions of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) in T-helper 1 cells (Th1 response) of B-CLL.

METHODS

Peripheral blood mononuclear cells from 21 healthy individuals and purified T cells from 21 early-stage and 15 late-stage B-CLL patients were activated with phorbol myristate acetate and ionomycin. The Th1 cytoplasmic cytokines were evaluated in CD4(+) and CD8(+) T cells by flow cytometry.

RESULTS

The percentages of CD4(+) and CD8(+) T cells positive for IL-2 were significantly lower in B-CLL patients than in healthy individuals (P = 0.030 and 0.049, respectively). No significant differences in TNF-alpha or IFN-gamma intracellular expressions were found between patients and healthy individuals. TNF-alpha- and IFN-gamma-expressing CD8 T cells were disease stage dependent, being significantly higher in late-stage patients (P < 0.001 for both cytokines).

CONCLUSIONS

Our present observations suggested that Th1 cytokines may be of major importance in the pathogenesis of B-CLL.

Authors+Show Affiliations

Service of Internal Medicine I, Hospital Universitario Puerta de Hierro, Universidad Autónoma, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14582134

Citation

Gallego, A, et al. "Production of Intracellular IL-2, TNF-alpha, and IFN-gamma By T Cells in B-CLL." Cytometry. Part B, Clinical Cytometry, vol. 56, no. 1, 2003, pp. 23-9.
Gallego A, Vargas JA, Castejón R, et al. Production of intracellular IL-2, TNF-alpha, and IFN-gamma by T cells in B-CLL. Cytometry B Clin Cytom. 2003;56(1):23-9.
Gallego, A., Vargas, J. A., Castejón, R., Citores, M. J., Romero, Y., Millán, I., & Durántez, A. (2003). Production of intracellular IL-2, TNF-alpha, and IFN-gamma by T cells in B-CLL. Cytometry. Part B, Clinical Cytometry, 56(1), 23-9.
Gallego A, et al. Production of Intracellular IL-2, TNF-alpha, and IFN-gamma By T Cells in B-CLL. Cytometry B Clin Cytom. 2003;56(1):23-9. PubMed PMID: 14582134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of intracellular IL-2, TNF-alpha, and IFN-gamma by T cells in B-CLL. AU - Gallego,A, AU - Vargas,J A, AU - Castejón,R, AU - Citores,M J, AU - Romero,Y, AU - Millán,I, AU - Durántez,A, PY - 2003/10/29/pubmed PY - 2004/2/10/medline PY - 2003/10/29/entrez SP - 23 EP - 9 JF - Cytometry. Part B, Clinical cytometry JO - Cytometry B Clin Cytom VL - 56 IS - 1 N2 - BACKGROUND: Recent evidence indicates that the slowly expanding population of CD5(+) B cells that characterizes B-cell chronic lymphocytic leukemia (B-CLL) could be related to defects in the response to cytokine produced by T cells that regulate apoptosis. We studied the intracellular expressions of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) in T-helper 1 cells (Th1 response) of B-CLL. METHODS: Peripheral blood mononuclear cells from 21 healthy individuals and purified T cells from 21 early-stage and 15 late-stage B-CLL patients were activated with phorbol myristate acetate and ionomycin. The Th1 cytoplasmic cytokines were evaluated in CD4(+) and CD8(+) T cells by flow cytometry. RESULTS: The percentages of CD4(+) and CD8(+) T cells positive for IL-2 were significantly lower in B-CLL patients than in healthy individuals (P = 0.030 and 0.049, respectively). No significant differences in TNF-alpha or IFN-gamma intracellular expressions were found between patients and healthy individuals. TNF-alpha- and IFN-gamma-expressing CD8 T cells were disease stage dependent, being significantly higher in late-stage patients (P < 0.001 for both cytokines). CONCLUSIONS: Our present observations suggested that Th1 cytokines may be of major importance in the pathogenesis of B-CLL. SN - 1552-4949 UR - https://www.unboundmedicine.com/medline/citation/14582134/Production_of_intracellular_IL_2_TNF_alpha_and_IFN_gamma_by_T_cells_in_B_CLL_ L2 - https://doi.org/10.1002/cyto.b.10052 DB - PRIME DP - Unbound Medicine ER -