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Effect of ets-related transcription factor (ERT) on transforming growth factor (TGF)-beta type II receptor gene expression in human cancer cell lines.
J Exp Clin Cancer Res. 2003 Sep; 22(3):477-80.JE

Abstract

Transcriptional repression of the TGF-beta type II receptor (RII) is one of the mechanisms leading to TGF-beta resistance. The newly identified epithelium-specific ets transcription factor ERT/ESX/ESE-1 binds to the TGF-beta RII promoter and induces promoter activity. This study aims to investigate the mechanisms underlying development of ERT-mediated TGF-beta resistance using antisense ERT oligonucleotide. We performed Northern blot analysis of TGF-beta RII expression in human colon cancer cell line, RKO, after transfecting these cells with MFG-antisense-ERT retroviral construct. The plasmid containing the chloramphenicol acetyltransferase (CAT) gene alone was used as the control. The amount of TGF-beta RII mRNA appears to be poor in RKO cells expressing antisense ERT compared with both parental RKO and control cells. In conclusion, transfection of MFG-antisense-ERT construct into the colon cancer cell line could result in lower levels of TGF-beta RII mRNA expression, which means that ERT mediates the expression of TGF-beta RII and the transcriptional inhibition of ERT could be a one of the mechanisms of colonic carcinogenesis. More in vitro and in vivo studies should be required to evaluate this treatment in clinical setting.

Authors+Show Affiliations

Dept. of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14582709

Citation

Lee, H J., et al. "Effect of Ets-related Transcription Factor (ERT) On Transforming Growth Factor (TGF)-beta Type II Receptor Gene Expression in Human Cancer Cell Lines." Journal of Experimental & Clinical Cancer Research : CR, vol. 22, no. 3, 2003, pp. 477-80.
Lee HJ, Chang JH, Kim YS, et al. Effect of ets-related transcription factor (ERT) on transforming growth factor (TGF)-beta type II receptor gene expression in human cancer cell lines. J Exp Clin Cancer Res. 2003;22(3):477-80.
Lee, H. J., Chang, J. H., Kim, Y. S., Kim, S. J., & Yang, H. K. (2003). Effect of ets-related transcription factor (ERT) on transforming growth factor (TGF)-beta type II receptor gene expression in human cancer cell lines. Journal of Experimental & Clinical Cancer Research : CR, 22(3), 477-80.
Lee HJ, et al. Effect of Ets-related Transcription Factor (ERT) On Transforming Growth Factor (TGF)-beta Type II Receptor Gene Expression in Human Cancer Cell Lines. J Exp Clin Cancer Res. 2003;22(3):477-80. PubMed PMID: 14582709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of ets-related transcription factor (ERT) on transforming growth factor (TGF)-beta type II receptor gene expression in human cancer cell lines. AU - Lee,H J, AU - Chang,J H, AU - Kim,Y S, AU - Kim,S J, AU - Yang,H K, PY - 2003/10/30/pubmed PY - 2004/7/21/medline PY - 2003/10/30/entrez SP - 477 EP - 80 JF - Journal of experimental & clinical cancer research : CR JO - J Exp Clin Cancer Res VL - 22 IS - 3 N2 - Transcriptional repression of the TGF-beta type II receptor (RII) is one of the mechanisms leading to TGF-beta resistance. The newly identified epithelium-specific ets transcription factor ERT/ESX/ESE-1 binds to the TGF-beta RII promoter and induces promoter activity. This study aims to investigate the mechanisms underlying development of ERT-mediated TGF-beta resistance using antisense ERT oligonucleotide. We performed Northern blot analysis of TGF-beta RII expression in human colon cancer cell line, RKO, after transfecting these cells with MFG-antisense-ERT retroviral construct. The plasmid containing the chloramphenicol acetyltransferase (CAT) gene alone was used as the control. The amount of TGF-beta RII mRNA appears to be poor in RKO cells expressing antisense ERT compared with both parental RKO and control cells. In conclusion, transfection of MFG-antisense-ERT construct into the colon cancer cell line could result in lower levels of TGF-beta RII mRNA expression, which means that ERT mediates the expression of TGF-beta RII and the transcriptional inhibition of ERT could be a one of the mechanisms of colonic carcinogenesis. More in vitro and in vivo studies should be required to evaluate this treatment in clinical setting. SN - 0392-9078 UR - https://www.unboundmedicine.com/medline/citation/14582709/Effect_of_ets_related_transcription_factor__ERT__on_transforming_growth_factor__TGF__beta_type_II_receptor_gene_expression_in_human_cancer_cell_lines_ L2 - http://www.diseaseinfosearch.org/result/2708 DB - PRIME DP - Unbound Medicine ER -