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Roles of CaMKII, PKA, and PKC in the induction and maintenance of LTP of C-fiber-evoked field potentials in rat spinal dorsal horn.
J Neurophysiol. 2004 Mar; 91(3):1122-33.JN

Abstract

Long-term potentiation (LTP) of C-fiber-evoked field potentials in spinal dorsal horn may be relevant to hyperalgesia, an increased response to noxious stimulation. The mechanism underlying this form of synaptic plasticity is, however, still unclear. Considerable evidence has shown that calcium/calmodulin-dependent protein kinase II (CaMKII), protein kinase A (PKA), and protein kinase C (PKC) are important for LTP in hippocampus. In this study, the roles of these three protein kinases in the induction and maintenance of LTP of C-fiber-evoked field potentials were evaluated by application of specific inhibitors of CaMKII (KN-93 and AIP), PKA (Rp-CPT-cAMPS), and PKC (chelerythrine and Gö 6983) at the recording segments before and after LTP induction in urethane-anesthetized Sprague-Dawley rats. We found both KN-93 and AIP, when applied at 30 min prior to tetanic stimulation, completely blocked LTP induction. At 30 min after LTP induction, KN-93 and AIP reversed LTP completely, and at 60 min after LTP induction, they depressed spinal LTP in most rats tested. Three hours after LTP induction, however, KN-93 or AIP did not affect the spinal LTP. Rp-CPT-cAMPS, chelerythrine, and Gö 6983 blocked the spinal LTP when applied at 30 min before tetanic stimulation and reversed LTP completely at 15 min after LTP induction. In contrast, at 30 min after LTP induction, the drugs never affected the spinal LTP. These results suggest that activation of CaMKII, PKA, and PKC may be crucial for the induction and the early-phase but not for the late-phase maintenance of the spinal LTP.

Authors+Show Affiliations

Department of Physiology, Zhongshan Medical School of Sun Yat-sen University, Guangzhou 510089, Peoples Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14586032

Citation

Yang, Hong-Wei, et al. "Roles of CaMKII, PKA, and PKC in the Induction and Maintenance of LTP of C-fiber-evoked Field Potentials in Rat Spinal Dorsal Horn." Journal of Neurophysiology, vol. 91, no. 3, 2004, pp. 1122-33.
Yang HW, Hu XD, Zhang HM, et al. Roles of CaMKII, PKA, and PKC in the induction and maintenance of LTP of C-fiber-evoked field potentials in rat spinal dorsal horn. J Neurophysiol. 2004;91(3):1122-33.
Yang, H. W., Hu, X. D., Zhang, H. M., Xin, W. J., Li, M. T., Zhang, T., Zhou, L. J., & Liu, X. G. (2004). Roles of CaMKII, PKA, and PKC in the induction and maintenance of LTP of C-fiber-evoked field potentials in rat spinal dorsal horn. Journal of Neurophysiology, 91(3), 1122-33.
Yang HW, et al. Roles of CaMKII, PKA, and PKC in the Induction and Maintenance of LTP of C-fiber-evoked Field Potentials in Rat Spinal Dorsal Horn. J Neurophysiol. 2004;91(3):1122-33. PubMed PMID: 14586032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Roles of CaMKII, PKA, and PKC in the induction and maintenance of LTP of C-fiber-evoked field potentials in rat spinal dorsal horn. AU - Yang,Hong-Wei, AU - Hu,Xiao-Dong, AU - Zhang,Hong-Mei, AU - Xin,Wen-Jun, AU - Li,Ming-Tao, AU - Zhang,Tong, AU - Zhou,Li-Jun, AU - Liu,Xian-Guo, Y1 - 2003/10/29/ PY - 2003/10/31/pubmed PY - 2004/4/14/medline PY - 2003/10/31/entrez SP - 1122 EP - 33 JF - Journal of neurophysiology JO - J. Neurophysiol. VL - 91 IS - 3 N2 - Long-term potentiation (LTP) of C-fiber-evoked field potentials in spinal dorsal horn may be relevant to hyperalgesia, an increased response to noxious stimulation. The mechanism underlying this form of synaptic plasticity is, however, still unclear. Considerable evidence has shown that calcium/calmodulin-dependent protein kinase II (CaMKII), protein kinase A (PKA), and protein kinase C (PKC) are important for LTP in hippocampus. In this study, the roles of these three protein kinases in the induction and maintenance of LTP of C-fiber-evoked field potentials were evaluated by application of specific inhibitors of CaMKII (KN-93 and AIP), PKA (Rp-CPT-cAMPS), and PKC (chelerythrine and Gö 6983) at the recording segments before and after LTP induction in urethane-anesthetized Sprague-Dawley rats. We found both KN-93 and AIP, when applied at 30 min prior to tetanic stimulation, completely blocked LTP induction. At 30 min after LTP induction, KN-93 and AIP reversed LTP completely, and at 60 min after LTP induction, they depressed spinal LTP in most rats tested. Three hours after LTP induction, however, KN-93 or AIP did not affect the spinal LTP. Rp-CPT-cAMPS, chelerythrine, and Gö 6983 blocked the spinal LTP when applied at 30 min before tetanic stimulation and reversed LTP completely at 15 min after LTP induction. In contrast, at 30 min after LTP induction, the drugs never affected the spinal LTP. These results suggest that activation of CaMKII, PKA, and PKC may be crucial for the induction and the early-phase but not for the late-phase maintenance of the spinal LTP. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/14586032/Roles_of_CaMKII_PKA_and_PKC_in_the_induction_and_maintenance_of_LTP_of_C_fiber_evoked_field_potentials_in_rat_spinal_dorsal_horn_ L2 - http://www.physiology.org/doi/full/10.1152/jn.00735.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -