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Flow cytometric determination of atypical antigen expression in acute leukemia for the study of minimal residual disease.
Cytometry. 1992; 13(8):893-901.C

Abstract

The aim of this study was to investigate to which extent acute leukemias could be monitored for residual disease by using atypical antigen combinations as leukemia-related markers. Atypical antigenic features were determined by double color flow cytometry and included coexpression of lymphoid and myeloid related antigens, unphysiological coexpression of immature and mature antigens, and lack of an antigen that is normally expressed during maturation. Atypical immunophenotypes were detected in 35 of 68 patients with AML (51.5%) and 15 of 24 patients with ALL (62.5%). When 12 patients with leukemia-associated markers were again analyzed at relapse, the relevant antigen combinations were retained in 11 of them. The sensitivity of this two color flow cytometric assay as determined in dilution experiments was 1 in 10(3) to 10(4) cells. Follow-up studies of bone marrow samples revealed that, after induction chemotherapy cells with leukemia-associated markers were detectable in several patients at a frequency of 0.5 to 4%, but only patients in whom the cells with atypical antigens never disappeared suffered from relapse. In contrast, patients who became negative for the atypical cells remained in complete remission (median remission duration after the first negative bone marrow assessment by flow cytometry 52 weeks, range 20-102). We conclude that atypical antigen combinations, which are present in a meaningful number of acute leukemias, are a valuable means of monitoring acute leukemia patients during follow-up. This flow cytometric approach can complement other strategies to get a more accurate definition of remission in acute leukemia.

Authors+Show Affiliations

Department of Internal Medicine, University of Innsbruck, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1459006

Citation

Drach, J, et al. "Flow Cytometric Determination of Atypical Antigen Expression in Acute Leukemia for the Study of Minimal Residual Disease." Cytometry, vol. 13, no. 8, 1992, pp. 893-901.
Drach J, Drach D, Glassl H, et al. Flow cytometric determination of atypical antigen expression in acute leukemia for the study of minimal residual disease. Cytometry. 1992;13(8):893-901.
Drach, J., Drach, D., Glassl, H., Gattringer, C., & Huber, H. (1992). Flow cytometric determination of atypical antigen expression in acute leukemia for the study of minimal residual disease. Cytometry, 13(8), 893-901.
Drach J, et al. Flow Cytometric Determination of Atypical Antigen Expression in Acute Leukemia for the Study of Minimal Residual Disease. Cytometry. 1992;13(8):893-901. PubMed PMID: 1459006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometric determination of atypical antigen expression in acute leukemia for the study of minimal residual disease. AU - Drach,J, AU - Drach,D, AU - Glassl,H, AU - Gattringer,C, AU - Huber,H, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 893 EP - 901 JF - Cytometry JO - Cytometry VL - 13 IS - 8 N2 - The aim of this study was to investigate to which extent acute leukemias could be monitored for residual disease by using atypical antigen combinations as leukemia-related markers. Atypical antigenic features were determined by double color flow cytometry and included coexpression of lymphoid and myeloid related antigens, unphysiological coexpression of immature and mature antigens, and lack of an antigen that is normally expressed during maturation. Atypical immunophenotypes were detected in 35 of 68 patients with AML (51.5%) and 15 of 24 patients with ALL (62.5%). When 12 patients with leukemia-associated markers were again analyzed at relapse, the relevant antigen combinations were retained in 11 of them. The sensitivity of this two color flow cytometric assay as determined in dilution experiments was 1 in 10(3) to 10(4) cells. Follow-up studies of bone marrow samples revealed that, after induction chemotherapy cells with leukemia-associated markers were detectable in several patients at a frequency of 0.5 to 4%, but only patients in whom the cells with atypical antigens never disappeared suffered from relapse. In contrast, patients who became negative for the atypical cells remained in complete remission (median remission duration after the first negative bone marrow assessment by flow cytometry 52 weeks, range 20-102). We conclude that atypical antigen combinations, which are present in a meaningful number of acute leukemias, are a valuable means of monitoring acute leukemia patients during follow-up. This flow cytometric approach can complement other strategies to get a more accurate definition of remission in acute leukemia. SN - 0196-4763 UR - https://www.unboundmedicine.com/medline/citation/1459006/Flow_cytometric_determination_of_atypical_antigen_expression_in_acute_leukemia_for_the_study_of_minimal_residual_disease_ L2 - https://doi.org/10.1002/cyto.990130813 DB - PRIME DP - Unbound Medicine ER -