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Donor locus selection during Saccharomyces cerevisiae mating type interconversion responds to distant regulatory signals.
Genetics. 1992 Dec; 132(4):929-42.G

Abstract

Mating type interconversion in homothallic strains of the yeast Saccharomyces cerevisiae results from directed transposition of a mating type allele from one of the two silent donor loci, HML and HMR, to the expressing locus, MAT. Cell type regulates the selection of the particular donor locus to be utilized during mating type interconversion: MATa cells preferentially select HML alpha and MAT alpha cells preferentially select HMRa. Such preferential selection indicates that the cell is able to distinguish between HML and HMR during mating type interconversion. Accordingly, we designed experiments to identify those features perceived by the cell to discriminate HML and HMR. We demonstrate that discrimination does not derive from the different structures of the HML and HMR loci, from the unique sequences flanking each donor locus nor from any of the DNA distal to the HM loci on chromosome III. Moreover, we find that the sequences flanking the MAT locus do not function in the preferential selection of one donor locus over the other. We propose that the positions of the donor loci on the left and right arms of chromosome III is the characteristic utilized by the cell to distinguish HML and HMR. This positional information is not generated by either CEN3 or the MAT locus, but probably derives from differences in the chromatin structure, chromosome folding or intranuclear localization of the two ends of chromosome III.

Links

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Authors+Show Affiliations

Weiler KS
Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
Broach JR
No affiliation info available

MeSH

Base SequenceDNA Mutational AnalysisDNA, FungalGene Expression Regulation, FungalMating FactorMolecular Sequence DataOligodeoxyribonucleotidesPeptidesSaccharomyces cerevisiaeTelomere

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1459444

Citation

Weiler, K S., and J R. Broach. "Donor Locus Selection During Saccharomyces Cerevisiae Mating Type Interconversion Responds to Distant Regulatory Signals." Genetics, vol. 132, no. 4, 1992, pp. 929-42.
Weiler KS, Broach JR. Donor locus selection during Saccharomyces cerevisiae mating type interconversion responds to distant regulatory signals. Genetics. 1992;132(4):929-42.
Weiler, K. S., & Broach, J. R. (1992). Donor locus selection during Saccharomyces cerevisiae mating type interconversion responds to distant regulatory signals. Genetics, 132(4), 929-42.
Weiler KS, Broach JR. Donor Locus Selection During Saccharomyces Cerevisiae Mating Type Interconversion Responds to Distant Regulatory Signals. Genetics. 1992;132(4):929-42. PubMed PMID: 1459444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Donor locus selection during Saccharomyces cerevisiae mating type interconversion responds to distant regulatory signals. AU - Weiler,K S, AU - Broach,J R, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 929 EP - 42 JF - Genetics JO - Genetics VL - 132 IS - 4 N2 - Mating type interconversion in homothallic strains of the yeast Saccharomyces cerevisiae results from directed transposition of a mating type allele from one of the two silent donor loci, HML and HMR, to the expressing locus, MAT. Cell type regulates the selection of the particular donor locus to be utilized during mating type interconversion: MATa cells preferentially select HML alpha and MAT alpha cells preferentially select HMRa. Such preferential selection indicates that the cell is able to distinguish between HML and HMR during mating type interconversion. Accordingly, we designed experiments to identify those features perceived by the cell to discriminate HML and HMR. We demonstrate that discrimination does not derive from the different structures of the HML and HMR loci, from the unique sequences flanking each donor locus nor from any of the DNA distal to the HM loci on chromosome III. Moreover, we find that the sequences flanking the MAT locus do not function in the preferential selection of one donor locus over the other. We propose that the positions of the donor loci on the left and right arms of chromosome III is the characteristic utilized by the cell to distinguish HML and HMR. This positional information is not generated by either CEN3 or the MAT locus, but probably derives from differences in the chromatin structure, chromosome folding or intranuclear localization of the two ends of chromosome III. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/1459444/Donor_locus_selection_during_Saccharomyces_cerevisiae_mating_type_interconversion_responds_to_distant_regulatory_signals_ DB - PRIME DP - Unbound Medicine ER -