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Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC-FL.
Biomed Chromatogr. 2003 Oct; 17(7):471-6.BC

Abstract

This paper describes highly sensitive HPLC methods for the determination of amphetamine (AP) and methamphetamine (MP) in abusers' plasma and hair samples. AP and MP were derivatized with the fluorescent reagent, DIB-Cl, to yield a highly fluorescent DIB-derivatives of AP and MP, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 and 430 nm, respectively. The separation was achieved on an ODS column with isocratic mobile phases composed of acetoniltrile and citrate buffer (55:45, v/v) for plasma samples and of acetonitrile-methanol-citrate buffer (45:20:37.5, v/v/v) for hair samples. The limits of detection were less than 0.87 ng/mL and 0.12 ng/mg in plasma and hair samples, respectively, for both AP and MP. The methods were then applied to the determination of MP and its metabolite AP in plasma obtained from two cases of illegally ingested MP and in one of the cases' hair received later. Case I was treated with dialysis; samples before and after dialysis were analyzed by the described method. After dialysis for 5 h, the total plasma levels of AP and MP decreased from 720 to 190 ng/mL. For case II, MP and AP levels were monitored for 3 days after digestion. Total plasma levels decreased from 57 ng/mL in the day of digestion to 11 ng/mL after 3 days. In hair samples, AP and MP could also be detected in very low concentrations.

Authors+Show Affiliations

Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14598332

Citation

Nakashima, Kenichiro, et al. "Determination of Methamphetamine and Amphetamine in Abusers' Plasma and Hair Samples With HPLC-FL." Biomedical Chromatography : BMC, vol. 17, no. 7, 2003, pp. 471-6.
Nakashima K, Kaddoumi A, Ishida Y, et al. Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC-FL. Biomed Chromatogr. 2003;17(7):471-6.
Nakashima, K., Kaddoumi, A., Ishida, Y., Itoh, T., & Taki, K. (2003). Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC-FL. Biomedical Chromatography : BMC, 17(7), 471-6.
Nakashima K, et al. Determination of Methamphetamine and Amphetamine in Abusers' Plasma and Hair Samples With HPLC-FL. Biomed Chromatogr. 2003;17(7):471-6. PubMed PMID: 14598332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC-FL. AU - Nakashima,Kenichiro, AU - Kaddoumi,Amal, AU - Ishida,Yukihiro, AU - Itoh,Takachika, AU - Taki,Kenji, PY - 2003/11/5/pubmed PY - 2004/7/22/medline PY - 2003/11/5/entrez SP - 471 EP - 6 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 17 IS - 7 N2 - This paper describes highly sensitive HPLC methods for the determination of amphetamine (AP) and methamphetamine (MP) in abusers' plasma and hair samples. AP and MP were derivatized with the fluorescent reagent, DIB-Cl, to yield a highly fluorescent DIB-derivatives of AP and MP, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 and 430 nm, respectively. The separation was achieved on an ODS column with isocratic mobile phases composed of acetoniltrile and citrate buffer (55:45, v/v) for plasma samples and of acetonitrile-methanol-citrate buffer (45:20:37.5, v/v/v) for hair samples. The limits of detection were less than 0.87 ng/mL and 0.12 ng/mg in plasma and hair samples, respectively, for both AP and MP. The methods were then applied to the determination of MP and its metabolite AP in plasma obtained from two cases of illegally ingested MP and in one of the cases' hair received later. Case I was treated with dialysis; samples before and after dialysis were analyzed by the described method. After dialysis for 5 h, the total plasma levels of AP and MP decreased from 720 to 190 ng/mL. For case II, MP and AP levels were monitored for 3 days after digestion. Total plasma levels decreased from 57 ng/mL in the day of digestion to 11 ng/mL after 3 days. In hair samples, AP and MP could also be detected in very low concentrations. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/14598332/Determination_of_methamphetamine_and_amphetamine_in_abusers'_plasma_and_hair_samples_with_HPLC_FL_ L2 - https://doi.org/10.1002/bmc.278 DB - PRIME DP - Unbound Medicine ER -