Tags

Type your tag names separated by a space and hit enter

Nitric oxide and collagen expression in allergic upper-airway disease.
Am J Rhinol. 2003 Sep-Oct; 17(5):269-74.AJ

Abstract

BACKGROUND

The presence of nitric oxide (NO) in high concentrations has been described in the nasal mucosa of patients with untreated allergic rhinitis. We sought to examine the role of exogenous, as well as endogenous, NO in the production of collagen type I and type III by human nasal fibroblasts.

METHODS

Primary cultured fibroblasts derived from eosinophilic nasal polyps were exposed to NO donors (500 microM of S-nitroso-N-acetyl-D,L-penicillamine (SNAP) 1000 micoM of 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene (DETA-NONOate)) and various other compounds over a 24-hour incubation period. Collagen production was evaluated qualitatively by immunocytochemistry and quantitatively by Western blot analysis.

RESULTS

Maximally stimulated fibroblasts established a 2,2-fold increase in the production of type III collagen relative type 1, as compared with baseline. Oxyhemoglobin, an NO scavenger, abolished this effect. SNAP (500 microM) caused a 15.68 +/- 0.68% increase in collagen type I synthesis as compared with unstimulated controls (p < 0.05). In contrast, incubation with SNAP caused an increase in collagen type III production by a factor of 34.68 +/- 0.32% (p < 0.05).

CONCLUSION

NO stimulates collagen expression in human nasal polyp-derived fibroblasts. This stimulation appeared to favor the up-regulation of collagen type III, leading to a shift in the ratio of collagen type I to type III production.

Authors+Show Affiliations

Tewfik MA
Department of Otolaryngology, Sir Mortimer B. Davis Jewish General Hospital, and Meakins-Christie Laboratories, Respiratory Division, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Bernardes JF
No affiliation info available
Shan J
No affiliation info available
Robinson M
No affiliation info available
Frenkiel S
No affiliation info available
Eidelman DH
No affiliation info available

MeSH

Blotting, WesternCell DivisionCell LineCell SurvivalCells, CulturedCollagenCollagenasesFibroblastsHumansImmunohistochemistryNasal MucosaNasal PolypsNitric OxideNitric Oxide DonorsPenicillamineRespiratory HypersensitivityTranscription, Genetic

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14599130

Citation

Tewfik, Marc A., et al. "Nitric Oxide and Collagen Expression in Allergic Upper-airway Disease." American Journal of Rhinology, vol. 17, no. 5, 2003, pp. 269-74.
Tewfik MA, Bernardes JF, Shan J, et al. Nitric oxide and collagen expression in allergic upper-airway disease. Am J Rhinol. 2003;17(5):269-74.
Tewfik, M. A., Bernardes, J. F., Shan, J., Robinson, M., Frenkiel, S., & Eidelman, D. H. (2003). Nitric oxide and collagen expression in allergic upper-airway disease. American Journal of Rhinology, 17(5), 269-74.
Tewfik MA, et al. Nitric Oxide and Collagen Expression in Allergic Upper-airway Disease. Am J Rhinol. 2003 Sep-Oct;17(5):269-74. PubMed PMID: 14599130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide and collagen expression in allergic upper-airway disease. AU - Tewfik,Marc A, AU - Bernardes,Julio F, AU - Shan,Jichuan, AU - Robinson,Michelle, AU - Frenkiel,Saul, AU - Eidelman,David H, PY - 2003/11/6/pubmed PY - 2004/3/10/medline PY - 2003/11/6/entrez SP - 269 EP - 74 JF - American journal of rhinology JO - Am J Rhinol VL - 17 IS - 5 N2 - BACKGROUND: The presence of nitric oxide (NO) in high concentrations has been described in the nasal mucosa of patients with untreated allergic rhinitis. We sought to examine the role of exogenous, as well as endogenous, NO in the production of collagen type I and type III by human nasal fibroblasts. METHODS: Primary cultured fibroblasts derived from eosinophilic nasal polyps were exposed to NO donors (500 microM of S-nitroso-N-acetyl-D,L-penicillamine (SNAP) 1000 micoM of 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene (DETA-NONOate)) and various other compounds over a 24-hour incubation period. Collagen production was evaluated qualitatively by immunocytochemistry and quantitatively by Western blot analysis. RESULTS: Maximally stimulated fibroblasts established a 2,2-fold increase in the production of type III collagen relative type 1, as compared with baseline. Oxyhemoglobin, an NO scavenger, abolished this effect. SNAP (500 microM) caused a 15.68 +/- 0.68% increase in collagen type I synthesis as compared with unstimulated controls (p < 0.05). In contrast, incubation with SNAP caused an increase in collagen type III production by a factor of 34.68 +/- 0.32% (p < 0.05). CONCLUSION: NO stimulates collagen expression in human nasal polyp-derived fibroblasts. This stimulation appeared to favor the up-regulation of collagen type III, leading to a shift in the ratio of collagen type I to type III production. SN - 1050-6586 UR - https://www.unboundmedicine.com/medline/citation/14599130/Nitric_oxide_and_collagen_expression_in_allergic_upper_airway_disease_ DB - PRIME DP - Unbound Medicine ER -