Tags

Type your tag names separated by a space and hit enter

CD4+CD25+ regulatory T cells generated in response to insulin B:9-23 peptide prevent adoptive transfer of diabetes by diabetogenic T cells.
J Autoimmun 2003; 21(3):221-37JA

Abstract

NOD mice have a relative deficiency of CD4+CD25+ regulatory T cells that could result in an inability to maintain peripheral tolerance. The aim of this study was to induce the generation of CD4+CD25+ regulatory T cells in response to autoantigens to prevent type 1 diabetes (T1D). We found that immunization of NOD mice with insulin B-chain peptide B:9-23 followed by 72 h in vitro culture with B:9-23 peptide induces generation of CD4+CD25+ regulatory T cells. Route of immunization has a critical role in the generation of these cells. Non-autoimmune mice BALB/c, C57BL/6 and NOR did not show up regulation of CD4+CD25+ regulatory T cells. These cells secreted large amounts of TGF-beta and TNF-alpha with little or no IFN-gamma and IL-10. Adoptive transfer of these CD4+CD25+ regulatory T cells into NOD-SCID mice completely prevented the adoptive transfer of disease by diabetogenic T cells. Although, non-self antigenic OVA (323-339) peptide immunization and in vitro culture with OVA (323-339) peptide does result in up regulation of CD4+CD25+ T cells, these cells did not prevent transfer of diabetes. Our study for the first time identified the generation of antigen-specific CD4+CD25+ regulatory T cells specifically in response to immunization with B:9-23 peptide in NOD mice that are capable of blocking adoptive transfer of diabetes. Our results suggest the possibility of using autoantigens to induce antigen-specific regulatory T cells to prevent and regulate autoimmune diabetes.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of Western Ontario, and John P. Robarts Research Institute, University of Western Ontario, London, N6A 5C1, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14599847

Citation

Mukherjee, Rinee, et al. "CD4+CD25+ Regulatory T Cells Generated in Response to Insulin B:9-23 Peptide Prevent Adoptive Transfer of Diabetes By Diabetogenic T Cells." Journal of Autoimmunity, vol. 21, no. 3, 2003, pp. 221-37.
Mukherjee R, Chaturvedi P, Qin HY, et al. CD4+CD25+ regulatory T cells generated in response to insulin B:9-23 peptide prevent adoptive transfer of diabetes by diabetogenic T cells. J Autoimmun. 2003;21(3):221-37.
Mukherjee, R., Chaturvedi, P., Qin, H. Y., & Singh, B. (2003). CD4+CD25+ regulatory T cells generated in response to insulin B:9-23 peptide prevent adoptive transfer of diabetes by diabetogenic T cells. Journal of Autoimmunity, 21(3), pp. 221-37.
Mukherjee R, et al. CD4+CD25+ Regulatory T Cells Generated in Response to Insulin B:9-23 Peptide Prevent Adoptive Transfer of Diabetes By Diabetogenic T Cells. J Autoimmun. 2003;21(3):221-37. PubMed PMID: 14599847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD4+CD25+ regulatory T cells generated in response to insulin B:9-23 peptide prevent adoptive transfer of diabetes by diabetogenic T cells. AU - Mukherjee,Rinee, AU - Chaturvedi,Pratibha, AU - Qin,Hui-Yu, AU - Singh,Bhagirath, PY - 2003/11/6/pubmed PY - 2004/6/30/medline PY - 2003/11/6/entrez SP - 221 EP - 37 JF - Journal of autoimmunity JO - J. Autoimmun. VL - 21 IS - 3 N2 - NOD mice have a relative deficiency of CD4+CD25+ regulatory T cells that could result in an inability to maintain peripheral tolerance. The aim of this study was to induce the generation of CD4+CD25+ regulatory T cells in response to autoantigens to prevent type 1 diabetes (T1D). We found that immunization of NOD mice with insulin B-chain peptide B:9-23 followed by 72 h in vitro culture with B:9-23 peptide induces generation of CD4+CD25+ regulatory T cells. Route of immunization has a critical role in the generation of these cells. Non-autoimmune mice BALB/c, C57BL/6 and NOR did not show up regulation of CD4+CD25+ regulatory T cells. These cells secreted large amounts of TGF-beta and TNF-alpha with little or no IFN-gamma and IL-10. Adoptive transfer of these CD4+CD25+ regulatory T cells into NOD-SCID mice completely prevented the adoptive transfer of disease by diabetogenic T cells. Although, non-self antigenic OVA (323-339) peptide immunization and in vitro culture with OVA (323-339) peptide does result in up regulation of CD4+CD25+ T cells, these cells did not prevent transfer of diabetes. Our study for the first time identified the generation of antigen-specific CD4+CD25+ regulatory T cells specifically in response to immunization with B:9-23 peptide in NOD mice that are capable of blocking adoptive transfer of diabetes. Our results suggest the possibility of using autoantigens to induce antigen-specific regulatory T cells to prevent and regulate autoimmune diabetes. SN - 0896-8411 UR - https://www.unboundmedicine.com/medline/citation/14599847/CD4+CD25+_regulatory_T_cells_generated_in_response_to_insulin_B:9_23_peptide_prevent_adoptive_transfer_of_diabetes_by_diabetogenic_T_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0896841103001148 DB - PRIME DP - Unbound Medicine ER -