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Detection of K-ras gene mutation at codon 12 by pancreatic duct brushing for pancreatic cancer.
Hepatobiliary Pancreat Dis Int. 2003 May; 2(2):313-7.HP

Abstract

OBJECTIVE

To assess the diagnostic value of endoscopic pancreatic duct brushing in detecting mutation of the K-ras gene at codon 12 in cytologic specimens from patients with pancreatic cancer.

METHODS

Thirty-five patients treated at Changhai Hospital, Shanghai between 1999 and 2001 were enrolled. Their cells obtained by pancreatic duct brushing during endoscopic retrograde cholangiopancreatography (ERCP) were suspended with phosphate buffer solution (PBS). DNA of the cells was extracted and mutation of the K-ras gene at codon 12 detected by means of PCR-SSCP.

RESULTS

The K-ras gene mutation rate of pancreatic cancer was 70%, which was higher than that of chronic pancreatitis (14%, P<0.05). K-ras gene mutation was not found in patients with pancreatic cystocarcinoma and duodenum carcinoma. As to the location of pancreatic cancer, no significant difference was observed between the head, the body and tail. The sensitivity, specificity, accuracy of pancreatic duct brushing in detecting pancreatic cancer was 70%, 94%, and 83%, respectively.

CONCLUSION

K-ras analysis of pancreatic brushing samples is helpful in the diagnosis of patients with early pancreatic cancer.

Authors+Show Affiliations

Department of Gastroenterology, Changhai Hospital, Second Military Medical Univercity, Shanghai 200433, China. drliuffeng@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

14599992

Citation

Liu, Feng, et al. "Detection of K-ras Gene Mutation at Codon 12 By Pancreatic Duct Brushing for Pancreatic Cancer." Hepatobiliary & Pancreatic Diseases International : HBPD INT, vol. 2, no. 2, 2003, pp. 313-7.
Liu F, Li ZS, Xu GM, et al. Detection of K-ras gene mutation at codon 12 by pancreatic duct brushing for pancreatic cancer. HBPD INT. 2003;2(2):313-7.
Liu, F., Li, Z. S., Xu, G. M., Sun, Z. X., Zhou, G. X., Ren, X. X., Tu, Z. X., & Gong, Y. F. (2003). Detection of K-ras gene mutation at codon 12 by pancreatic duct brushing for pancreatic cancer. Hepatobiliary & Pancreatic Diseases International : HBPD INT, 2(2), 313-7.
Liu F, et al. Detection of K-ras Gene Mutation at Codon 12 By Pancreatic Duct Brushing for Pancreatic Cancer. HBPD INT. 2003;2(2):313-7. PubMed PMID: 14599992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of K-ras gene mutation at codon 12 by pancreatic duct brushing for pancreatic cancer. AU - Liu,Feng, AU - Li,Zhao-Shen, AU - Xu,Guo-Ming, AU - Sun,Zhen-Xing, AU - Zhou,Guo-Xiong, AU - Ren,Xue-Xin, AU - Tu,Zhen-Xing, AU - Gong,Yan-Fang, PY - 2003/11/6/pubmed PY - 2004/1/6/medline PY - 2003/11/6/entrez SP - 313 EP - 7 JF - Hepatobiliary & pancreatic diseases international : HBPD INT JO - HBPD INT VL - 2 IS - 2 N2 - OBJECTIVE: To assess the diagnostic value of endoscopic pancreatic duct brushing in detecting mutation of the K-ras gene at codon 12 in cytologic specimens from patients with pancreatic cancer. METHODS: Thirty-five patients treated at Changhai Hospital, Shanghai between 1999 and 2001 were enrolled. Their cells obtained by pancreatic duct brushing during endoscopic retrograde cholangiopancreatography (ERCP) were suspended with phosphate buffer solution (PBS). DNA of the cells was extracted and mutation of the K-ras gene at codon 12 detected by means of PCR-SSCP. RESULTS: The K-ras gene mutation rate of pancreatic cancer was 70%, which was higher than that of chronic pancreatitis (14%, P<0.05). K-ras gene mutation was not found in patients with pancreatic cystocarcinoma and duodenum carcinoma. As to the location of pancreatic cancer, no significant difference was observed between the head, the body and tail. The sensitivity, specificity, accuracy of pancreatic duct brushing in detecting pancreatic cancer was 70%, 94%, and 83%, respectively. CONCLUSION: K-ras analysis of pancreatic brushing samples is helpful in the diagnosis of patients with early pancreatic cancer. SN - 1499-3872 UR - https://www.unboundmedicine.com/medline/citation/14599992/Detection_of_K_ras_gene_mutation_at_codon_12_by_pancreatic_duct_brushing_for_pancreatic_cancer_ L2 - http://www.diseaseinfosearch.org/result/5551 DB - PRIME DP - Unbound Medicine ER -