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Heterogeneous expression and association of beta-catenin, p16 and c-myc in multistage colorectal tumorigenesis and progression detected by tissue microarray.
Int J Cancer. 2003 Dec 20; 107(6):896-902.IJ

Abstract

Most colorectal carcinomas (CRCs) arise from adenomas through an archetypal pathogenic pathway, the adenoma-carcinoma-metastasis sequence. Aberrant expression of beta-catenin, p16, E-cadherin and c-myc appears to have played important roles in the development and/or progression of CRC, but their precise distribution pattern and associations in different pathologic loci along CRC's pathogenic pathway have not been thoroughly examined. In this study, a tissue microarray (TMA) containing 85 advanced CRCs in different Dukes stages was constructed. In each of 85 cases, tissue specimens from normal mucosa and primary carcinomas in different layers of the bowel wall were included in the TMA. Tissue specimens from matched adenoma, lymph node metastases and distant metastases were obtained from 22, 21 and 21 cases, respectively. Expression patterns of beta-catenin, p16, E-cadherin and c-myc were evaluated by immunohistochemistry. The results revealed that nuclear expression of beta-catenin, p16 and c-myc was quantitatively increased from normal mucosa to premalignant adenoma, primary carcinoma and lymph node metastatic carcinoma; the frequency of nuclear overexpression of beta-catenin and p16 in lymph node metastases was significantly higher than that in distant metastases (p < 0.05). These results suggest an association between nuclear overexpression of beta-catenin and/or p16 and CRC lymph node metastasis but not distant metastasis. The results also showed that correlative high nuclear expression of beta-catenin and c-myc was observed in primary carcinomas involving the serosa and lymph node metastases (p < 0.05) but not in other pathologic regions of CRCs, suggesting that the tumor microenvironment in different pathologic loci of colorectal tumorigenesis and progression may influence c-myc responsiveness to beta-catenin/Tcf activation.

Authors+Show Affiliations

Department of Clinical Oncology, 1/F Building, School of Chinese Medicine, University of Hong Kong, 10 Sassoon Road, Hong Kong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14601048

Citation

Xie, Dan, et al. "Heterogeneous Expression and Association of Beta-catenin, P16 and C-myc in Multistage Colorectal Tumorigenesis and Progression Detected By Tissue Microarray." International Journal of Cancer, vol. 107, no. 6, 2003, pp. 896-902.
Xie D, Sham JS, Zeng WF, et al. Heterogeneous expression and association of beta-catenin, p16 and c-myc in multistage colorectal tumorigenesis and progression detected by tissue microarray. Int J Cancer. 2003;107(6):896-902.
Xie, D., Sham, J. S., Zeng, W. F., Lin, H. L., Che, L. H., Wu, H. X., Wen, J. M., Fang, Y., Hu, L., & Guan, X. Y. (2003). Heterogeneous expression and association of beta-catenin, p16 and c-myc in multistage colorectal tumorigenesis and progression detected by tissue microarray. International Journal of Cancer, 107(6), 896-902.
Xie D, et al. Heterogeneous Expression and Association of Beta-catenin, P16 and C-myc in Multistage Colorectal Tumorigenesis and Progression Detected By Tissue Microarray. Int J Cancer. 2003 Dec 20;107(6):896-902. PubMed PMID: 14601048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterogeneous expression and association of beta-catenin, p16 and c-myc in multistage colorectal tumorigenesis and progression detected by tissue microarray. AU - Xie,Dan, AU - Sham,Jonathan S T, AU - Zeng,Wei-Fen, AU - Lin,Han-Liang, AU - Che,Li-Hong, AU - Wu,Hui-Xi, AU - Wen,Jian-Ming, AU - Fang,Yan, AU - Hu,Liang, AU - Guan,Xin-Yuan, PY - 2003/11/6/pubmed PY - 2004/1/15/medline PY - 2003/11/6/entrez SP - 896 EP - 902 JF - International journal of cancer JO - Int. J. Cancer VL - 107 IS - 6 N2 - Most colorectal carcinomas (CRCs) arise from adenomas through an archetypal pathogenic pathway, the adenoma-carcinoma-metastasis sequence. Aberrant expression of beta-catenin, p16, E-cadherin and c-myc appears to have played important roles in the development and/or progression of CRC, but their precise distribution pattern and associations in different pathologic loci along CRC's pathogenic pathway have not been thoroughly examined. In this study, a tissue microarray (TMA) containing 85 advanced CRCs in different Dukes stages was constructed. In each of 85 cases, tissue specimens from normal mucosa and primary carcinomas in different layers of the bowel wall were included in the TMA. Tissue specimens from matched adenoma, lymph node metastases and distant metastases were obtained from 22, 21 and 21 cases, respectively. Expression patterns of beta-catenin, p16, E-cadherin and c-myc were evaluated by immunohistochemistry. The results revealed that nuclear expression of beta-catenin, p16 and c-myc was quantitatively increased from normal mucosa to premalignant adenoma, primary carcinoma and lymph node metastatic carcinoma; the frequency of nuclear overexpression of beta-catenin and p16 in lymph node metastases was significantly higher than that in distant metastases (p < 0.05). These results suggest an association between nuclear overexpression of beta-catenin and/or p16 and CRC lymph node metastasis but not distant metastasis. The results also showed that correlative high nuclear expression of beta-catenin and c-myc was observed in primary carcinomas involving the serosa and lymph node metastases (p < 0.05) but not in other pathologic regions of CRCs, suggesting that the tumor microenvironment in different pathologic loci of colorectal tumorigenesis and progression may influence c-myc responsiveness to beta-catenin/Tcf activation. SN - 0020-7136 UR - https://www.unboundmedicine.com/medline/citation/14601048/Heterogeneous_expression_and_association_of_beta_catenin_p16_and_c_myc_in_multistage_colorectal_tumorigenesis_and_progression_detected_by_tissue_microarray_ L2 - https://doi.org/10.1002/ijc.11514 DB - PRIME DP - Unbound Medicine ER -