Tags

Type your tag names separated by a space and hit enter

L chain isotype regulation in horse. I. Characterization of Ig lambda genes.
J Immunol. 1992 Dec 15; 149(12):3927-36.JI

Abstract

Analysis of 10 cDNA encoding lambda L chains of horse Ig indicated that this species may employ a relatively small number of variable region (V lambda) genes in the splenic B cell population. The V lambda sequences of all of the cDNA analyzed were closely related (> 88% identity at the nucleotide level) and were characterized by a deletion of the amino acid residue at position 3 compared with V lambda sequences so far described in other species. The 10 V lambda sequences could be grouped into three groups, V lambda 1 to V lambda 3, on the basis of a number of linked substitutions. Sequences within the groups showed the greatest divergence in the third cdr regions and at the V-J junctions. The junctional variation included amino acid substitutions on both sides of the V-J junction as well as the insertion or deletion of two to four amino acid residues. Four C lambda genes were identified in genomic blots of horse DNA, and three of these were found expressed in splenic cDNA. The fourth C lambda gene may represent a pseudogene, inasmuch as the associated J region possessed a defective heptamer joining sequence. Six of the nine possible V lambda-C lambda combinations were found in the cDNA analyzed, suggesting that genes belonging to groups V lambda 1 through V lambda 3 may rearrange to any one of three J lambda-C lambda genes. One V lambda germline gene was characterized and found to represent a distinct V lambda group (V lambda 4), not represented in the cDNA sequences analyzed. The number of germline V lambda genes was estimated to be 20 to 30, based on analysis of restriction fragments hybridizing with V lambda probes. On the basis of these data, we propose that the V lambda repertoire in horse may consist of relatively limited number of genes, of which only a few may be used at high frequency in the splenic B cell population. The results indicate that predominance of lambda-chains in horse Ig may not simply be due to the presence of a large germline V lambda gene repertoire.

Authors+Show Affiliations

Département de Biochimie, Université de Sherbrooke, Quebec, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1460283

Citation

Home, W A., et al. "L Chain Isotype Regulation in Horse. I. Characterization of Ig Lambda Genes." Journal of Immunology (Baltimore, Md. : 1950), vol. 149, no. 12, 1992, pp. 3927-36.
Home WA, Ford JE, Gibson DM. L chain isotype regulation in horse. I. Characterization of Ig lambda genes. J Immunol. 1992;149(12):3927-36.
Home, W. A., Ford, J. E., & Gibson, D. M. (1992). L chain isotype regulation in horse. I. Characterization of Ig lambda genes. Journal of Immunology (Baltimore, Md. : 1950), 149(12), 3927-36.
Home WA, Ford JE, Gibson DM. L Chain Isotype Regulation in Horse. I. Characterization of Ig Lambda Genes. J Immunol. 1992 Dec 15;149(12):3927-36. PubMed PMID: 1460283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - L chain isotype regulation in horse. I. Characterization of Ig lambda genes. AU - Home,W A, AU - Ford,J E, AU - Gibson,D M, PY - 1992/12/15/pubmed PY - 1992/12/15/medline PY - 1992/12/15/entrez SP - 3927 EP - 36 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 149 IS - 12 N2 - Analysis of 10 cDNA encoding lambda L chains of horse Ig indicated that this species may employ a relatively small number of variable region (V lambda) genes in the splenic B cell population. The V lambda sequences of all of the cDNA analyzed were closely related (> 88% identity at the nucleotide level) and were characterized by a deletion of the amino acid residue at position 3 compared with V lambda sequences so far described in other species. The 10 V lambda sequences could be grouped into three groups, V lambda 1 to V lambda 3, on the basis of a number of linked substitutions. Sequences within the groups showed the greatest divergence in the third cdr regions and at the V-J junctions. The junctional variation included amino acid substitutions on both sides of the V-J junction as well as the insertion or deletion of two to four amino acid residues. Four C lambda genes were identified in genomic blots of horse DNA, and three of these were found expressed in splenic cDNA. The fourth C lambda gene may represent a pseudogene, inasmuch as the associated J region possessed a defective heptamer joining sequence. Six of the nine possible V lambda-C lambda combinations were found in the cDNA analyzed, suggesting that genes belonging to groups V lambda 1 through V lambda 3 may rearrange to any one of three J lambda-C lambda genes. One V lambda germline gene was characterized and found to represent a distinct V lambda group (V lambda 4), not represented in the cDNA sequences analyzed. The number of germline V lambda genes was estimated to be 20 to 30, based on analysis of restriction fragments hybridizing with V lambda probes. On the basis of these data, we propose that the V lambda repertoire in horse may consist of relatively limited number of genes, of which only a few may be used at high frequency in the splenic B cell population. The results indicate that predominance of lambda-chains in horse Ig may not simply be due to the presence of a large germline V lambda gene repertoire. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1460283/L_chain_isotype_regulation_in_horse__I__Characterization_of_Ig_lambda_genes_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=1460283 DB - PRIME DP - Unbound Medicine ER -