Tags

Type your tag names separated by a space and hit enter

Changes in the fatty acid composition and hydroxyproline content in rat lung in relation to collagen synthesis after paraquat administration.
Fukushima J Med Sci 2003; 49(1):33-43FJ

Abstract

OBJECTIVES

Effect of paraquat on the fatty acid composition (weight percentage) of rat lung was studied with particular reference to the change of hydroxyproline content in the course of paraquat-induced dysfunction and subsequent repair.

METHODS

Eight-week-old male Wistar rats were administered paraquat at 20 mg/kg body weight subcutaneously, and the wet weight, hydroxyproline content and fatty acid composition of lungs of each group rats were analyzed at 2, 7, 14 or 28 days after treatment, respectively.

RESULTS

The percentage of palmitic acid (C16:0), arachidonic acid (C20:4) and docosahexaenoic acid (C22:6) significantly increased, and the percentage of oleic acid (C18:1) and the ratio of monounsaturated fatty acids/saturated fatty acids (M/S) significantly decreased comparing to control on day 28 after paraquat administration. The time-course of each fatty acid was observed for 28 days after paraquat administration. M/S ratio decreased after paraquat administration up to the 28th day, but the polyunsaturated fatty acids/saturated fatty acids (P/S) ratio decreased during the first 7 days, followed by a increase, and then reached higher level than the 0 day control at the 28th day. Hydroxyproline also increased between the 14th and the 28th days. Eicosapentaenoic acid (C20:5) had once increased during the first 2 days and decreased gradually, while C20:4 maintained high level in this period. C22:6 increased after paraquat administration and maintained high level up to the 28th day. This result indicated that desaturation and elongation in n-3 series fatty acids were accelerated after paraquat treatment, and consequently C20:5 was rapidly converted into C22:6 and decreased.

CONCLUSIONS

Paraquat might cause elevation of unsaturated fatty acids, espe- cially C20:4 but not C20:5 by the stimulation of the fatty acid desaturase system, and could consequently stimulate local collagen synthesis by C20:4 metabolites in the healing stage.

Authors+Show Affiliations

Department of Hygiene & Preventive Medicine, Fukushima Medical University School of Medicine, Fukushima, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14603950

Citation

Fukushima, Tetsuhito, et al. "Changes in the Fatty Acid Composition and Hydroxyproline Content in Rat Lung in Relation to Collagen Synthesis After Paraquat Administration." Fukushima Journal of Medical Science, vol. 49, no. 1, 2003, pp. 33-43.
Fukushima T, Tanaka K, Lim H, et al. Changes in the fatty acid composition and hydroxyproline content in rat lung in relation to collagen synthesis after paraquat administration. Fukushima J Med Sci. 2003;49(1):33-43.
Fukushima, T., Tanaka, K., Lim, H., & Moriyama, M. (2003). Changes in the fatty acid composition and hydroxyproline content in rat lung in relation to collagen synthesis after paraquat administration. Fukushima Journal of Medical Science, 49(1), pp. 33-43.
Fukushima T, et al. Changes in the Fatty Acid Composition and Hydroxyproline Content in Rat Lung in Relation to Collagen Synthesis After Paraquat Administration. Fukushima J Med Sci. 2003;49(1):33-43. PubMed PMID: 14603950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in the fatty acid composition and hydroxyproline content in rat lung in relation to collagen synthesis after paraquat administration. AU - Fukushima,Tetsuhito, AU - Tanaka,Keiko, AU - Lim,Heejin, AU - Moriyama,Masaki, PY - 2003/11/8/pubmed PY - 2003/12/3/medline PY - 2003/11/8/entrez SP - 33 EP - 43 JF - Fukushima journal of medical science JO - Fukushima J Med Sci VL - 49 IS - 1 N2 - OBJECTIVES: Effect of paraquat on the fatty acid composition (weight percentage) of rat lung was studied with particular reference to the change of hydroxyproline content in the course of paraquat-induced dysfunction and subsequent repair. METHODS: Eight-week-old male Wistar rats were administered paraquat at 20 mg/kg body weight subcutaneously, and the wet weight, hydroxyproline content and fatty acid composition of lungs of each group rats were analyzed at 2, 7, 14 or 28 days after treatment, respectively. RESULTS: The percentage of palmitic acid (C16:0), arachidonic acid (C20:4) and docosahexaenoic acid (C22:6) significantly increased, and the percentage of oleic acid (C18:1) and the ratio of monounsaturated fatty acids/saturated fatty acids (M/S) significantly decreased comparing to control on day 28 after paraquat administration. The time-course of each fatty acid was observed for 28 days after paraquat administration. M/S ratio decreased after paraquat administration up to the 28th day, but the polyunsaturated fatty acids/saturated fatty acids (P/S) ratio decreased during the first 7 days, followed by a increase, and then reached higher level than the 0 day control at the 28th day. Hydroxyproline also increased between the 14th and the 28th days. Eicosapentaenoic acid (C20:5) had once increased during the first 2 days and decreased gradually, while C20:4 maintained high level in this period. C22:6 increased after paraquat administration and maintained high level up to the 28th day. This result indicated that desaturation and elongation in n-3 series fatty acids were accelerated after paraquat treatment, and consequently C20:5 was rapidly converted into C22:6 and decreased. CONCLUSIONS: Paraquat might cause elevation of unsaturated fatty acids, espe- cially C20:4 but not C20:5 by the stimulation of the fatty acid desaturase system, and could consequently stimulate local collagen synthesis by C20:4 metabolites in the healing stage. SN - 0016-2590 UR - https://www.unboundmedicine.com/medline/citation/14603950/Changes_in_the_fatty_acid_composition_and_hydroxyproline_content_in_rat_lung_in_relation_to_collagen_synthesis_after_paraquat_administration_ L2 - http://japanlinkcenter.org/DN/JST.JSTAGE/fms/49.33?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -