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Anthocyanin profile of Korean cultivated kidney bean (Phaseolus vulgaris L.).
J Agric Food Chem. 2003 Nov 19; 51(24):7040-3.JA

Abstract

This investigation was conducted to determine the structures and amounts of anthocyanins obtained from seed coats of kidney bean (Phaseolus vulgaris L.) cultivated in Korea. Anthocyanins in the seed coat of kidney bean were extracted with 1% HCl/20% CH(3)OH, and the crude anthocyanin extracts were purified by semipreparative HPLC. Five major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-vis, LC/ES-MS, and 1H and 13C NMR). The structures of these five anthocyanins were elucidated as cyanidin 3,5-diglucoside, delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, and pelargonidin 3-glucoside. Using RP-HPLC with photodiode array detection, each of the five anthocyanins was separated within 12 min by using a gradient elution. It was proved that the application of RP-HPLC could be an excellent method for determining the composition and contents of anthocyanins in kidney bean. The preponderance of pelargonidin 3-glucoside and delphinidin 3-glucoside are observed in red and black kidney beans, respectively. However, in this study, it is reported for the first time that the contents and composition of anthocyanins in speckled seed depend on the classes of speckle color. The contents of cyanidin 3,5-diglucoside, delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, pelargonidin 3-glucoside, and total anthocyanins in seed coats of 16 kidney beans cultivated in Korea were in the ranges of 0-0.04, 0-2.61, 0-0.12, 0-0.17, 0-0.59 and 0-2.78 mg/g of dried seed coats, respectively.

Authors+Show Affiliations

Department of Pharmacognosy Material Development, Samcheok National University, Samcheok 245-711, Korea. cmg7004@samcheok.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14611168

Citation

Choung, Myoung-Gun, et al. "Anthocyanin Profile of Korean Cultivated Kidney Bean (Phaseolus Vulgaris L.)." Journal of Agricultural and Food Chemistry, vol. 51, no. 24, 2003, pp. 7040-3.
Choung MG, Choi BR, An YN, et al. Anthocyanin profile of Korean cultivated kidney bean (Phaseolus vulgaris L.). J Agric Food Chem. 2003;51(24):7040-3.
Choung, M. G., Choi, B. R., An, Y. N., Chu, Y. H., & Cho, Y. S. (2003). Anthocyanin profile of Korean cultivated kidney bean (Phaseolus vulgaris L.). Journal of Agricultural and Food Chemistry, 51(24), 7040-3.
Choung MG, et al. Anthocyanin Profile of Korean Cultivated Kidney Bean (Phaseolus Vulgaris L.). J Agric Food Chem. 2003 Nov 19;51(24):7040-3. PubMed PMID: 14611168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anthocyanin profile of Korean cultivated kidney bean (Phaseolus vulgaris L.). AU - Choung,Myoung-Gun, AU - Choi,Byoung-Rourl, AU - An,Young-Nam, AU - Chu,Yong-Ha, AU - Cho,Young-Son, PY - 2003/11/13/pubmed PY - 2004/1/14/medline PY - 2003/11/13/entrez SP - 7040 EP - 3 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 51 IS - 24 N2 - This investigation was conducted to determine the structures and amounts of anthocyanins obtained from seed coats of kidney bean (Phaseolus vulgaris L.) cultivated in Korea. Anthocyanins in the seed coat of kidney bean were extracted with 1% HCl/20% CH(3)OH, and the crude anthocyanin extracts were purified by semipreparative HPLC. Five major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-vis, LC/ES-MS, and 1H and 13C NMR). The structures of these five anthocyanins were elucidated as cyanidin 3,5-diglucoside, delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, and pelargonidin 3-glucoside. Using RP-HPLC with photodiode array detection, each of the five anthocyanins was separated within 12 min by using a gradient elution. It was proved that the application of RP-HPLC could be an excellent method for determining the composition and contents of anthocyanins in kidney bean. The preponderance of pelargonidin 3-glucoside and delphinidin 3-glucoside are observed in red and black kidney beans, respectively. However, in this study, it is reported for the first time that the contents and composition of anthocyanins in speckled seed depend on the classes of speckle color. The contents of cyanidin 3,5-diglucoside, delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, pelargonidin 3-glucoside, and total anthocyanins in seed coats of 16 kidney beans cultivated in Korea were in the ranges of 0-0.04, 0-2.61, 0-0.12, 0-0.17, 0-0.59 and 0-2.78 mg/g of dried seed coats, respectively. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/14611168/Anthocyanin_profile_of_Korean_cultivated_kidney_bean__Phaseolus_vulgaris_L___ DB - PRIME DP - Unbound Medicine ER -