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Rheumatoid arthritis synovial fluid macrophages express decreased tumor necrosis factor-related apoptosis-inducing ligand R2 and increased decoy receptor tumor necrosis factor-related apoptosis-inducing ligand R3.
Arthritis Rheum. 2003 Nov; 48(11):3096-101.AR

Abstract

OBJECTIVE

To characterize the expression pattern of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its cognate receptors (TRAIL R1, R2, R3, and R4) on rheumatoid arthritis (RA) synovial fluid (SF) lymphocytes and monocyte/macrophages and on cultured RA synovial fibroblasts.

METHODS

The expression of TRAIL and TRAIL receptors on RA SF lymphocytes and monocyte/macrophages, normal macrophages, and RA synovial fibroblasts was examined by flow cytometry with previously characterized monoclonal antibodies. The ability of adenoviral-mediated delivery of TRAIL to induce macrophage or RA synovial fibroblast apoptosis was examined by flow cytometry.

RESULTS

By flow cytometry, neither TRAIL nor its cognate receptors was detectable on RA SF lymphocytes or RA synovial fibroblasts. In contrast, RA SF macrophages expressed TRAIL R3, a decoy receptor (P < 0.01 versus isotype control), but not TRAIL, or TRAIL R1, R2, or R4. Normal peripheral blood-derived monocyte-differentiated macrophages expressed TRAIL R2 (P < 0.01), but not TRAIL or the other TRAIL receptors. Adenoviral-mediated delivery of TRAIL had no effect on the survival of normal macrophages or RA synovial fibroblasts but readily induced apoptosis in the prostate cancer cell line (PC-3) that expressed TRAIL R1 and R2.

CONCLUSION

TRAIL R1 and R2, which are required for signal transmission by TRAIL, were not detected on RA SF lymphocytes, macrophages, or synovial fibroblasts. These observations do not support a potential therapeutic role for TRAIL in RA.

Authors+Show Affiliations

Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14613271

Citation

Perlman, Harris, et al. "Rheumatoid Arthritis Synovial Fluid Macrophages Express Decreased Tumor Necrosis Factor-related Apoptosis-inducing Ligand R2 and Increased Decoy Receptor Tumor Necrosis Factor-related Apoptosis-inducing Ligand R3." Arthritis and Rheumatism, vol. 48, no. 11, 2003, pp. 3096-101.
Perlman H, Nguyen N, Liu H, et al. Rheumatoid arthritis synovial fluid macrophages express decreased tumor necrosis factor-related apoptosis-inducing ligand R2 and increased decoy receptor tumor necrosis factor-related apoptosis-inducing ligand R3. Arthritis Rheum. 2003;48(11):3096-101.
Perlman, H., Nguyen, N., Liu, H., Eslick, J., Esser, S., Walsh, K., Moore, T. L., & Pope, R. M. (2003). Rheumatoid arthritis synovial fluid macrophages express decreased tumor necrosis factor-related apoptosis-inducing ligand R2 and increased decoy receptor tumor necrosis factor-related apoptosis-inducing ligand R3. Arthritis and Rheumatism, 48(11), 3096-101.
Perlman H, et al. Rheumatoid Arthritis Synovial Fluid Macrophages Express Decreased Tumor Necrosis Factor-related Apoptosis-inducing Ligand R2 and Increased Decoy Receptor Tumor Necrosis Factor-related Apoptosis-inducing Ligand R3. Arthritis Rheum. 2003;48(11):3096-101. PubMed PMID: 14613271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rheumatoid arthritis synovial fluid macrophages express decreased tumor necrosis factor-related apoptosis-inducing ligand R2 and increased decoy receptor tumor necrosis factor-related apoptosis-inducing ligand R3. AU - Perlman,Harris, AU - Nguyen,Nadine, AU - Liu,Hongtao, AU - Eslick,Joy, AU - Esser,Sybille, AU - Walsh,Kenneth, AU - Moore,Terry L, AU - Pope,Richard M, PY - 2003/11/13/pubmed PY - 2003/12/3/medline PY - 2003/11/13/entrez SP - 3096 EP - 101 JF - Arthritis and rheumatism JO - Arthritis Rheum VL - 48 IS - 11 N2 - OBJECTIVE: To characterize the expression pattern of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its cognate receptors (TRAIL R1, R2, R3, and R4) on rheumatoid arthritis (RA) synovial fluid (SF) lymphocytes and monocyte/macrophages and on cultured RA synovial fibroblasts. METHODS: The expression of TRAIL and TRAIL receptors on RA SF lymphocytes and monocyte/macrophages, normal macrophages, and RA synovial fibroblasts was examined by flow cytometry with previously characterized monoclonal antibodies. The ability of adenoviral-mediated delivery of TRAIL to induce macrophage or RA synovial fibroblast apoptosis was examined by flow cytometry. RESULTS: By flow cytometry, neither TRAIL nor its cognate receptors was detectable on RA SF lymphocytes or RA synovial fibroblasts. In contrast, RA SF macrophages expressed TRAIL R3, a decoy receptor (P < 0.01 versus isotype control), but not TRAIL, or TRAIL R1, R2, or R4. Normal peripheral blood-derived monocyte-differentiated macrophages expressed TRAIL R2 (P < 0.01), but not TRAIL or the other TRAIL receptors. Adenoviral-mediated delivery of TRAIL had no effect on the survival of normal macrophages or RA synovial fibroblasts but readily induced apoptosis in the prostate cancer cell line (PC-3) that expressed TRAIL R1 and R2. CONCLUSION: TRAIL R1 and R2, which are required for signal transmission by TRAIL, were not detected on RA SF lymphocytes, macrophages, or synovial fibroblasts. These observations do not support a potential therapeutic role for TRAIL in RA. SN - 0004-3591 UR - https://www.unboundmedicine.com/medline/citation/14613271/Rheumatoid_arthritis_synovial_fluid_macrophages_express_decreased_tumor_necrosis_factor_related_apoptosis_inducing_ligand_R2_and_increased_decoy_receptor_tumor_necrosis_factor_related_apoptosis_inducing_ligand_R3_ L2 - https://doi.org/10.1002/art.11302 DB - PRIME DP - Unbound Medicine ER -