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Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts.
Cell Tissue Res. 2004 Feb; 315(2):157-66.CT

Abstract

In the central nervous system (CNS) complex endothelial tight junctions (TJs) form a restrictive paracellular diffusion barrier, the blood-brain barrier (BBB). Pathogenic changes within the CNS are frequently accompanied by the loss of BBB properties, resulting in brain edema. In order to investigate whether BBB leakiness can be monitored by a loss of TJ proteins from cellular borders, we used an in vitro BBB model where brain endothelial cells in co-culture with astrocytes form a tight permeability barrier for 3H-inulin and 14C-sucrose. Removal of astrocytes from the co-culture resulted in an increased permeability to small tracers across the brain endothelial cell monolayer and an opening of the TJs to horseradish peroxidase as detected by electron microscopy. Strikingly, opening of the endothelial TJs was not accompanied by any visible change in the molecular composition of endothelial TJs as junctional localization of the TJ-associated proteins claudin-3, claudin-5, occludin, ZO-1 or ZO-2 or the adherens junction-associated proteins beta-catenin or p120cas did not change. Thus, opening of BBB TJs is not readily accompanied by the complete loss of the junctional localization of TJ proteins.

Authors+Show Affiliations

Max Planck Institute for Physiological and Clinical Research, Bad Nauheim, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14615934

Citation

Hamm, Stefan, et al. "Astrocyte Mediated Modulation of Blood-brain Barrier Permeability Does Not Correlate With a Loss of Tight Junction Proteins From the Cellular Contacts." Cell and Tissue Research, vol. 315, no. 2, 2004, pp. 157-66.
Hamm S, Dehouck B, Kraus J, et al. Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts. Cell Tissue Res. 2004;315(2):157-66.
Hamm, S., Dehouck, B., Kraus, J., Wolburg-Buchholz, K., Wolburg, H., Risau, W., Cecchelli, R., Engelhardt, B., & Dehouck, M. P. (2004). Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts. Cell and Tissue Research, 315(2), 157-66.
Hamm S, et al. Astrocyte Mediated Modulation of Blood-brain Barrier Permeability Does Not Correlate With a Loss of Tight Junction Proteins From the Cellular Contacts. Cell Tissue Res. 2004;315(2):157-66. PubMed PMID: 14615934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts. AU - Hamm,Stefan, AU - Dehouck,Bénédicte, AU - Kraus,Jörg, AU - Wolburg-Buchholz,Karen, AU - Wolburg,Hartwig, AU - Risau,Werner, AU - Cecchelli,Roméo, AU - Engelhardt,Britta, AU - Dehouck,Marie-Pierre, Y1 - 2003/11/13/ PY - 2003/05/16/received PY - 2003/10/10/accepted PY - 2003/11/15/pubmed PY - 2004/11/16/medline PY - 2003/11/15/entrez SP - 157 EP - 66 JF - Cell and tissue research JO - Cell Tissue Res. VL - 315 IS - 2 N2 - In the central nervous system (CNS) complex endothelial tight junctions (TJs) form a restrictive paracellular diffusion barrier, the blood-brain barrier (BBB). Pathogenic changes within the CNS are frequently accompanied by the loss of BBB properties, resulting in brain edema. In order to investigate whether BBB leakiness can be monitored by a loss of TJ proteins from cellular borders, we used an in vitro BBB model where brain endothelial cells in co-culture with astrocytes form a tight permeability barrier for 3H-inulin and 14C-sucrose. Removal of astrocytes from the co-culture resulted in an increased permeability to small tracers across the brain endothelial cell monolayer and an opening of the TJs to horseradish peroxidase as detected by electron microscopy. Strikingly, opening of the endothelial TJs was not accompanied by any visible change in the molecular composition of endothelial TJs as junctional localization of the TJ-associated proteins claudin-3, claudin-5, occludin, ZO-1 or ZO-2 or the adherens junction-associated proteins beta-catenin or p120cas did not change. Thus, opening of BBB TJs is not readily accompanied by the complete loss of the junctional localization of TJ proteins. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/14615934/Astrocyte_mediated_modulation_of_blood_brain_barrier_permeability_does_not_correlate_with_a_loss_of_tight_junction_proteins_from_the_cellular_contacts_ L2 - https://dx.doi.org/10.1007/s00441-003-0825-y DB - PRIME DP - Unbound Medicine ER -