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Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics.
Anal Chem. 2003 Nov 15; 75(22):6196-201.AC

Abstract

The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH(2))(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microscopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.

Authors+Show Affiliations

Advanced Biomedical Science and Technology Group, Oak Ridge National Laboratory, Bethel Valley Road, MS-6101 PO Box 2008, Oak Ridge, Tennessee 37831-6101, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

14616001

Citation

Culha, Mustafa, et al. "Surface-enhanced Raman Scattering Substrate Based On a Self-assembled Monolayer for Use in Gene Diagnostics." Analytical Chemistry, vol. 75, no. 22, 2003, pp. 6196-201.
Culha M, Stokes D, Allain LR, et al. Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics. Anal Chem. 2003;75(22):6196-201.
Culha, M., Stokes, D., Allain, L. R., & Vo-Dinh, T. (2003). Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics. Analytical Chemistry, 75(22), 6196-201.
Culha M, et al. Surface-enhanced Raman Scattering Substrate Based On a Self-assembled Monolayer for Use in Gene Diagnostics. Anal Chem. 2003 Nov 15;75(22):6196-201. PubMed PMID: 14616001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics. AU - Culha,Mustafa, AU - Stokes,David, AU - Allain,Leonardo R, AU - Vo-Dinh,Tuan, PY - 2003/11/18/pubmed PY - 2004/6/3/medline PY - 2003/11/18/entrez SP - 6196 EP - 201 JF - Analytical chemistry JO - Anal Chem VL - 75 IS - 22 N2 - The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH(2))(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microscopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/14616001/Surface_enhanced_Raman_scattering_substrate_based_on_a_self_assembled_monolayer_for_use_in_gene_diagnostics_ L2 - https://doi.org/10.1021/ac0346003 DB - PRIME DP - Unbound Medicine ER -