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Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers.
J Appl Genet. 2003; 44(4):459-71.JA

Abstract

Wheat bread-making quality is closely correlated with composition and quantity of gluten proteins, in particular with high-molecular weight (HMW) glutenin subunits encoded by the Glu-1 genes. A multiplex polymerase chain reaction (PCR) method was developed to identify the allele composition of HMW glutenin complex Glu-1 loci (Glu-A1, Glu-B1 and Glu-D1) in common wheat genotypes. The study of multiplex PCR to obtain a well-balanced set of amplicons involved examination of various combinations of selected primer sets and/or thermal cycling conditions. One to three simultaneously amplified DNA fragments of HMW glutenin Glu-1 genes were separated by agarose slab-gel electrophoresis and differences between Ax1, Ax2* and Axnull genes of Glu-A1 loci, Bx6, Bx7 and Bx17 of Glu-B1, and Dx2, Dx5 and Dy10 genes of Glu-D1 loci were revealed. A complete agreement was found in identification of HMW glutenin subunits by both multiplex PCR analysis and SDS-PAGE for seventy-six Polish cultivars/strains of both spring and winter common wheat. Rapid identification of molecular markers of Glu-1 alleles by multiplex PCR can be an efficient alternative to the standard separation procedure for early selection of useful wheat genotypes with good bread-making quality.

Authors+Show Affiliations

Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14617825

Citation

Moczulski, Marcin, and Bolesław P. Salmanowicz. "Multiplex PCR Identification of Wheat HMW Glutenin Subunit Genes By Allele-specific Markers." Journal of Applied Genetics, vol. 44, no. 4, 2003, pp. 459-71.
Moczulski M, Salmanowicz BP. Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers. J Appl Genet. 2003;44(4):459-71.
Moczulski, M., & Salmanowicz, B. P. (2003). Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers. Journal of Applied Genetics, 44(4), 459-71.
Moczulski M, Salmanowicz BP. Multiplex PCR Identification of Wheat HMW Glutenin Subunit Genes By Allele-specific Markers. J Appl Genet. 2003;44(4):459-71. PubMed PMID: 14617825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers. AU - Moczulski,Marcin, AU - Salmanowicz,Bolesław P, PY - 2003/11/18/pubmed PY - 2004/1/31/medline PY - 2003/11/18/entrez SP - 459 EP - 71 JF - Journal of applied genetics JO - J Appl Genet VL - 44 IS - 4 N2 - Wheat bread-making quality is closely correlated with composition and quantity of gluten proteins, in particular with high-molecular weight (HMW) glutenin subunits encoded by the Glu-1 genes. A multiplex polymerase chain reaction (PCR) method was developed to identify the allele composition of HMW glutenin complex Glu-1 loci (Glu-A1, Glu-B1 and Glu-D1) in common wheat genotypes. The study of multiplex PCR to obtain a well-balanced set of amplicons involved examination of various combinations of selected primer sets and/or thermal cycling conditions. One to three simultaneously amplified DNA fragments of HMW glutenin Glu-1 genes were separated by agarose slab-gel electrophoresis and differences between Ax1, Ax2* and Axnull genes of Glu-A1 loci, Bx6, Bx7 and Bx17 of Glu-B1, and Dx2, Dx5 and Dy10 genes of Glu-D1 loci were revealed. A complete agreement was found in identification of HMW glutenin subunits by both multiplex PCR analysis and SDS-PAGE for seventy-six Polish cultivars/strains of both spring and winter common wheat. Rapid identification of molecular markers of Glu-1 alleles by multiplex PCR can be an efficient alternative to the standard separation procedure for early selection of useful wheat genotypes with good bread-making quality. SN - 1234-1983 UR - https://www.unboundmedicine.com/medline/citation/14617825/Multiplex_PCR_identification_of_wheat_HMW_glutenin_subunit_genes_by_allele_specific_markers_ DB - PRIME DP - Unbound Medicine ER -